DAGLB (NM_001142936) Human Untagged Clone

CAT#: SC326054

DAGLB (untagged)-Human diacylglycerol lipase, beta (DAGLB), transcript variant 2



  "NM_001142936" in other vectors (6)

CNY 8,740.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DAGLB rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DAGLBETA; KCCR13L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC326054 representing NM_001142936.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCGGGGATGGTACTCTTCGGCCGGCGCTGGGCCATCGCCAGCGACGACTTGGTCTTCCCAGGGTTC
TTCGAGCTGGTCGTGCGAGTGCTGTGGTGGATTGGCATTCTGACGTTGTATCTCATGCACAGAGGAAAG
CTGGACTGTGCTGGTGGAGCCTTGCTCAGCAGTTACTTGATCGTCCTCATGATTCTCCTGGCAGTTGTC
ATATGTACTGTGTCAGCCATCATGTGTGTCAGCATGAGAGGAACGATTTGTAACCCTGGACCGCGGAAG
TCTATGTCTAAGCTGCTTTACATCCGCCTGGCGCTGTTTTTTCCAGAGATGGTCTGGGCCTCTCTGGGG
GCTGCCTGGGTGGCAGATGGTGTTCAGTGCGACAGGACAGTTGTAAACGGCATCATCGCAACCGTCGTG
GTCAGGACACAGATCTGGTGCCCAGCGACATTGCGGCGGGCCTCGCCCTGCTTCATCAGCAACAGGACA
ATATCAGGAACAACCAAGAGCCTGCCCAGGTGGTCTGCCATGCCCCAGGGAGCTCCCAGCTGCAGAAGC
AGAACCACAGACTATGACTTGGTCGGAGGCGATCAGCTCAACTGTCACTTCGGCTCCATCCTGCACACC
ACAGGGCTGCAGTACAGGGACTTCATCCACGTCAGCTTCCATGACAAGGTTTACGAGCTGCCGTTTTTA
GTGGCTCTGGATCACAGGAAAGAGTCTGTTGTGGTCGCTGTGAGGGGGACCATGTCTCTGCAGGATGTC
CTTACGGACCTGTCAGCGGAGAGTGAGGTGCTGGACGTGGAGTGTGAGGTGCAGGACCGCCTGGCACAC
AAGGGTATTTCTCAAGCTGCCAGATACGTTTACCAACGACTCATCAACGACGGGATTTTGAGCCAAGCC
TTCAGCATTGCTCCTGAGTACCGGCTGGTCATAGTGGGCCACAGCCTCGGGGGCGGGGCGGCCGCCCTG
CTGGCCACCATGCTCAGAGCCGCCTACCCGCAGGTCAGGTGCTACGCCTTCTCCCCACCCCGGGGGCTG
TGGAGCAAAGCTCTGCAGGAATATTCTCAGAGCTTCATCGTGTCACTCGTCCTGGGGAAGGATGTGATT
CCCAGGCTCAGTGTGACCAACTTGGAAGATCTGAAGAGAAGAATCTTGCGAGTGGTCGCGCACTGCAAT
AAACCCAAGTACAAGATCTTGCTGCACGGTTTGTGGTACGAACTGTTTGGAGGAAACCCCAACAACTTG
CCCACGGAGCTGGACGGGGGCGACCAGGAAGTCCTGACACAGCCTCTTCTGGGGGAGCAGAGCCTACTG
ACGCGCTGGTCCCCGGCCTACAGCTTCTCCAGCGACTCCCCACTGGACTCTTCTCCCAAGTACCCCCCT
CTCTACCCTCCCGGCAGGATCATCCACCTGCAGGAGGAGGGCGCCTCGGGGCGGTTTGGCTGCTGCTCT
GCTGCTCACTATAGCGCCAAGTGGTCACACGAAGCGGAATTCAGCAAAATACTCATAGGTCCGAAGATG
CTCACCGACCACATGCCAGACATCCTGATGCGGGCCTTGGACAGCGTGGTCTCCGACAGAGCGGCCTGC
GTCTCCTGTCCAGCACAAGGGGTCTCCAGTGTGGACGTGGCCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001142936
Insert Size 1632 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001142936.1
RefSeq Size 2523 bp
RefSeq ORF 1632 bp
Locus ID 221955
UniProt ID Q8NCG7
Protein Families Transmembrane
MW 59.8 kDa
Gene Summary Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks two alternate internal exons, compared to variant 1, resulting in a translational frameshift in the mid coding region. The encoded isoform (2) has the same N- and C-termini but is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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