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NGLY1 (NM_001145293) Human Untagged Clone
CAT＃: SC326128
NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 2
CNY 10,260.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDDG; CDG1V; PNG-1; PNG1; PNGase |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326128 representing NM_001145293.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCGGCGGCATTGGGCAGCTCCTCAGGCTCGGCGTCCCCGGCCGTGGCTGAGCTCTGCCAGAAC ACCCCGGAGACCTTTTTGGAGGCCTCCAAGCTGCTGCTCACCTATGCTGACAACATCCTCAGAAACCCT AATGATGAAAAATATAGATCCATCCGGATTGGAAACACAGCCTTTTCTACTAGACTCTTGCCTGTCAGA GGAGCTGTTGAATGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACATCTCATCTTTCCTAAAAAA GCTTCAGTGGAGCAGCTGCAAAAAATTCGTGACCTGATTGCCATAGAGAGAAGTAGCAGACTGGATGGC TCAAATAAGAGCCACAAAGTAAAGTCATCTCAGCAACCTGCAGCCAGTACCCAGCTTCCTACAACACCA TCTTCAAATCCCAGTGGGTTAAACCAGCACACAAGGAACCGTCAAGGGCAGTCATCAGATCCACCATCT GCTTCAACGGTTGCTGCTGACTCAGCCATTCTAGAAGTTCTTCAGTCCAACATTCAGCATGTGCTGGTC TATGAAAATCCTGCTCTTCAGGAGAAAGCGTTGGCTTGTATTCCGGTCCAAGAACTAAAAAGGAAATCA CAAGAAAAGTTATCGAGAGCTAGAAAATTGGATAAAGGTATCAATATAAGTGATGAGGATTTTCTTTTG CTGGAGCTTTTGCACTGGTTTAAGGAAGAATTTTTTCACTGGGTGAATAACGTTTTGTGCAGCAAATGT GGTGGACAGACTAGGTCTAGAGATAGATCATTACTGCCCAGTGATGATGAGCTGAAGTGGGGTGCAAAG GAAGTGGAAGATCATTACTGTGATGCCTGCCAGTTCAGCAATCGATTCCCAAGATATAATAACCCTGAG AAACTTTTGGAAACAAGATGTGGACGGTGTGGCGAGTGGGCCAATTGTTTTACACTGTGCTGCCGAGCT GTAGGGTTTGAAGCTCGCTATGTTTGGGATTACACAGAGTTACAGCGCACATTAAGCCTAAAACTGACC ACGTTAAAGGAGATTGGAAAAACATTTTTAAGGATTTCAAAGAGACAGAAATTAATACAGGTAGTTGAT GTCACTTGGCGATATTCCTGCAAACATGAAGAGGTGATTGCCAGAAGAACTAAGGTTAAAGAAGCATTA CTTCGAGACACTATTAATGGGCTTAATAAGCAGAGGCAACTGTTTTTGTCAGAAAACAGAAGGAAAGAA CTTCTCCAGAGGATAATTGTGGAGCTTGTTGAATTTATATCTCCCAAAACCCCTAAACCTGGAGAACTT GGGGGAAGAATATCTGGGTCAGTGGCTTGGAGAGTAGCCCGAGGTGAAATGGGTCTACAGAGAAAAGAA ACCTTGTTTATTCCCTGTGAAAATGAGAAGATTTCTAAACAGCTCCACCTTTGTTACAATATTGTGAAA GATCGTTATGTTCGAGTTTCAAATAACAATCAAACCATTTCTGGATGGGAGAATGGCGTGTGGAAAATG GAATCTATATTCAGAAAAGTTGAAACAGACTGGCACATGGTATATTTGGCCCGAAAGGAAGGATCATCT TTTGCTTATATTTCCTGGAAGTTTGAGTGTGGGTCAGTTGGCCTAAAAGTAGATAGCATTTCTATTAGA ACAAGTAGTCAAACTTTTCAGACTGGAACAGTAGAATGGAAATTGCGATCTGATACAGCACAAGTAGAA CTGACAGGCGATAACAGTCTTCACTCCTATGCTGATTTTTCTGGTGCCACTGAAGTTATTTTGGAAGCA GAATTAAGCAGAGGAGATGGTGATGTCGCTTGGCAACACACCCAGCTGTTTAGACAAAGCTTAAATGAC CATGAAGAAAATTGTTTGGAGATAATTATAAAATTCAGTGACCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001145293 |
| Insert Size | 1911 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145293.1 |
| RefSeq Size | 2545 bp |
| RefSeq ORF | 1911 bp |
| Locus ID | 55768 |
| UniProt ID | Q96IV0 |
| MW | 72.3 kDa |
| Gene Summary | This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009] Transcript Variant: This variant (2) includes an alternate exon that causes a frameshift and lacks an alternate exon which restores the reading frame, compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227404 | NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 2 |
CNY 4,736.00 |
|
| RC227404L3 | Lenti-ORF clone of NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 2 |
CNY 5,890.00 |
|
| RC227404L4 | Lenti-ORF clone of NGLY1 (mGFP-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 2 |
CNY 7,136.00 |
|
| RG227404 | NGLY1 (tGFP-tagged) - Human N-glycanase 1 (NGLY1), transcript variant 2 |
CNY 4,370.00 |
