GIT2 (NM_001135214) Human Untagged Clone

CAT#: SC326202

GIT2 (untagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 5



  "NM_001135214" in other vectors (4)

CNY 13,970.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-GIT2 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CAT-2; CAT2; PKL
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_001135214, the custom clone sequence may differ by one or more nucleotides
ATGTCGAAACGGCTCCGGAGCAGCGAGGTGTGCGCTGACTGCAGCGGGCCGGATCCTTCC
TGGGCATCAGTAAATAGGGGAACGTTTTTATGTGATGAGTGCTGCAGTGTCCATCGGAGT
CTAGGGCGCCATATCTCCCAAGTGAGGCATCTGAAACACACACCGTGGCCTCCAACACTG
CTTCAGATGGTTGAGACCTTGTATAATAACGGTGCTAACTCTATATGGGAGCATTCTTTG
CTGGACCCTGCGTCTATTATGAGTGGAAGACGTAAAGCTAATCCACAGGATAAAGTACAT
CCCAATAAAGCGGAATTCATCAGAGCCAAGTATCAGATGTTAGCGTTCGTCCATCGCTTG
CCCTGCCGGGATGACGATAGTGTGACTGCCAAAGATCTTAGCAAGCAACTCCATTCGAGC
GTGAGAACAGGGAATCTTGAAACCTGTTTGAGACTGTTATCTTTAGGAGCACAAGCCAAC
TTCTTTCATCCTGAAAAAGGAAACACCCCACTCCATGTTGCCTCCAAAGCAGGGCAGATT
TTACAGGCTGAATTATTGGCAGTATATGGAGCAGACCCAGGCACACAGGATTCTAGTGGG
AAAACTCCCGTTGATTATGCAAGGCAAGGAGGGCACCATGAGCTGGCAGAGCGCCTCGTG
GAAATACAGTATGAGCTAACGGACAGACTAGCCTTCTATCTCTGTGGCAGGAAACCAGAT
CACAAAAATGGACAGCACTTTATAATACCTCAAATGGCAGACAGCAGCCTGGATTTGTCT
GAATTGGCAAAAGCTGCTAAGAAGAAACTTCAATCTCTAAGTAATCATTTGTTTGAAGAA
CTTGCCATGGATGTGTACGATGAAGTTGACAGGCGAGAGACGGATGCAGTCTGGCTTGCC
ACGCAAAACCACAGCGCCCTGGTAACCGAGACAACGGTCGTCCCCTTTCTTCCGGTCAAT
CCTGAGTACTCATCAACACGAAATCAGGGCAGACAGAAGTTAGCTCGGTTCAACGCCCAT
GAGTTTGCCACGCTGGTCATTGACATTCTCAGTGACGCCAAGAGGAGACAGCAGGGCAGT
TCTCTCTCGGGTTCAAAAGACAATGTGGAGCTCATACTGAAAACCATCAATAACCAGCAC
AGCGTTGAGAGTCAAGACAACGATCAGCCCGACTATGACAGCGTGGCATCAGACGAAGAC
ACAGATTTGGAAACCACTGCAAGCAAAACAAACCGGCAGAAGAGCCTAGATTCAGATTTA
TCAGATGGACCAGTCACTGTACAGGAATTTATGGAGGTCAAAAACGCTCTAGTGGCTTCT
GAGGCCAAGATACAGCAGCTAATGAAGGTGAATAACAACTTGAGTGACGAGCTGAGAATT
ATGCAGAAAAAGCTTCAAACACTCCAGAGTGAAAATTCGAACCTCAGGAAACAGGCCACA
ACCAATGTATATCAGGTGCAAACTGGTTCTGAGTACACAGACACTTCCAACCACTCTTCC
TTAAAGAGACGTCCGTCTGCCCGGGGCAGTAGGCCCATGTCCATGTACGAGACCGGATCA
GGTCAGAAACCATATCTCCCAATGGGAGAAGCGAGCCGCCCCGAAGAGAGCAGGATGAGA
CTCCAGCCCTTCCCCGCGCACGCATCCAGGCTGGAGAAGCAGAACAGCACACCTGAGAGT
GACTACGACAACACTCCCAACGACATGGAGCCAGATGGCATGGGGTCAAGCCGAAAGGGA
CGGCAAAGAAGTATGGTGTGGCCAGGGGATGGCTTGGTACCAGACACAGCAGAACCCCAT
GTGGCCCCAAGCCCCACTCTCCCTAGCACCGAAGATGTCATCAGGAAGACTGAACAGATC
ACCAAAAACATACAGGAGCTCTTAAGAGCAGCCCAAGAAAATAAACATGACAGTTATATT
CCCTGCTCAGAGAGGATACACGTAGCTGTTACAGAAATGGCAGCATTATTCCCCAAAAAA
CCCAAGTCTGATATGGTGAGGACTTCCCTTCGTTTACTGACGTCCAGTGCCTACCGACTG
CAGTCAGAGTGCAAGAAGACCCTCCCAGGGGACCCCGGCTCACCCACAGACGTTCAGCTG
GTCACGCAGCAGGTCATCCAGTGTGCGTACGACATCGCCAAGGCTGCCAAGCAGCTGGTT
ACCATCACCACCAAAGAGAACAACAAC
Restriction Sites Please inquire     
ACCN NM_001135214
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001135214.1, NP_001128686.1
RefSeq Size 5553 bp
RefSeq ORF 2190 bp
Locus ID 9815
UniProt ID Q14161
Protein Families Druggable Genome
Protein Pathways Endocytosis
Gene Summary This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008]
Transcript Variant: This variant (5) lacks one in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (5) is missing one fragment but has the same N- and C-termini as isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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