Dysferlin (DYSF) (NM_001130976) Human Untagged Clone

CAT#: SC326422

DYSF (untagged)-Human dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant 9



  "NM_001130976" in other vectors (2)

CNY 39,710.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (4)
DYSF Antibody - middle region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms FER1L1; LGMD2B; LGMDR2; MMD1
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_001130976, the custom clone sequence may differ by one or more nucleotides
ATGCTGAGGGTCTTCATCCTCTATGCCGAGAACGTCCACACACCCGACACCGACATCAGC
GATGCCTACTGCTCCGCGGTGTTTGCAGGGGTGAAGAAGAGAACCAAAGTCATCAAGAAC
AGCGTGAACCCTGTATGGAATGAGGGATTTGAATGGGACCTCAAGGGCATCCCCCTGGAC
CAGGGCTCTGAGCTTCATGTGGTGGTCAAAGACCATGAGACGATGGGGAGGAACAGGTTC
CTGGGGGAAGCCAAGGTCCCACTCCGAGAGGTCCTCGCCACCCCTAGTCTGTCCGCCAGC
TTCAATGCCCCCCTGCTGGACACCAAGAAGCAGCCCACAGGGGCCTCGCTGGTCCTGCAG
GTGTCCTACACACCGCTGCCTGGAGCTGTGCCCCTGTTCCCGCCCCCTACTCCTCTGGAG
CCCTCCCCGACTCTGCCTGACCTGGATGTAGTGGCAGACACAGGAGGAGAGGAAGACACA
GAGGACCAGGGACTCACTGGAGATGAGGCGGAGCCATTCCTGGATCAAAGCGGAGGCCCG
GGGGCTCCCACCACCCCAAGGAAACTACCTTCACGTCCTCCGCCCCACTACCCCGGGATC
AAAAGAAAGCGAAGTGCGCCTACATCTAGAAAGCTGCTGTCAGACAAACCGCAGGATTTC
CAGATCAGGGTCCAGGTGATCGAGGGGCGCCAGCTGCCGGGGGTGAACATCAAGCCTGTG
GTCAAGGTTACCGCTGCAGGGCAGACCAAGCGGACGCGGATCCACAAGGGAAACAGCCCA
CTCTTCAATGAGACTCTTTTCTTCAACTTGTTTGACTCTCCTGGGGAGCTGTTTGATGAG
CCCATCTTTATCACGGTGGTAGACTCTCGTTCTCTCAGGACAGATGCTCTCCTCGGGGAG
TTCCGGATGGACGTGGGCACCATTTACAGAGAGCCCCGGCACGCCTATCTCAGGAAGTGG
CTGCTGCTCTCAGACCCTGATGACTTCTCTGCTGGGGCCAGAGGCTACCTGAAAACAAGC
CTTTGTGTGCTGGGGCCTGGGGACGAAGCGCCTCTGGAGAGAAAAGACCCCTCTGAAGAC
AAGGAGGACATTGAAAGCAACCTGCTCCGGCCCACAGGCGTAGCCCTGCGAGGAGCCCAC
TTCTGCCTGAAGGTCTTCCGGGCCGAGGACTTGCCGCAGATGGACGATGCCGTGATGGAC
AACGTGAAACAGATCTTTGGCTTCGAGAGTAACAAGAAGAACTTGGTGGACCCCTTTGTG
GAGGTCAGCTTTGCGGGGAAAATGCTGTGCAGCAAGATCTTGGAGAAGACGGCCAACCCT
CAGTGGAACCAGAACATCACACTGCCTGCCATGTTTCCCTCCATGTGCGAAAAAATGAGG
ATTCGTATCATAGACTGGGACCGCCTGACTCACAATGACATCGTGGCTACCACCTACCTG
AGTATGTCGAAAATCTCTGCCCCTGGAGGAGAAATAGAAGTGGATGACTACCTGGGCTTC
CTCCCCACTTTTGGGCCCTGCTACATCAACCTCTATGGCAGTCCCAGAGAGTTCACAGGC
TTCCCAGACCCCTACACAGAGCTCAACACAGGCAAGGGGGAAGGTGTGGCTTATCGTGGC
CGGCTTCTGCTCTCCCTGGAGACCAAGCTGGTGGAGCACAGTGAACAGAAGGTGGAGGAC
CTTCCTGCGGATGACATCCTCCGGGTGGAGAAGTACCTTAGGAGGCGCAAGTACTCCCTG
TTTGCGGCCTTCTACTCAGCCACCATGCTGCAGGATGTGGATGATGCCATCCAGTTTGAG
GTCAGCATCGGGAACTACGGGAACAAGTTCGACATGACCTGCCTGCCGCTGGCCTCCACC
ACTCAGTACAGCCGTGCAGTCTTTGACGGGTGCCACTACTACTACCTACCCTGGGGTAAC
GTGAAACCTGTGGTGGTGCTGTCATCCTACTGGGAGGACATCAGCCATAGAATCGAGACT
CAGAACCAGCTGCTTGGGATTGCTGACCGGCTGGAAGCTGGCCTGGAGCAGGTCCACCTG
GCCCTGAAGGCGCAGTGCTCCACGGAGGACGTGGACTCGCTGGTGGCTCAGCTGACGGAT
GAGCTCATCGCAGGCTGCAGCCAGCCTCTGGGTGACATCCATGAGACACCCTCTGCCACC
CACCTGGACCAGTACCTGTACCAGCTGCGCACCCATCACCTGAGCCAAATCACTGAGGCT
GCCCTGGCCCTGAAGCTCGGCCACAGTGAGCTCCCTGCAGCTCTGGAGCAGGCGGAGGAC
TGGCTCCTGCGTCTGCGTGCCCTGGCAGAGGAGCCCCAGAACAGCCTGCCGGACATCGTC
ATCTGGATGCTGCAGGGAGACAAGCGTGTGGCATACCAGCGGGTGCCCGCCCACCAAGTC
CTCTTCTCCCGGCGGGGTGCCAACTACTGTGGCAAGAATTGTGGGAAGCTACAGACAATC
TTTCTGAAATATCCGATGGAGAAGGTGCCTGGCGCCCGGATGCCAGTGCAGATACGGGTC
AAGCTGTGGTTTGGGCTCTCAGTGGATGAGAAGGAGTTCAACCAGTTTGCTGAGGGGAAG
CTGTCTGTCTTTGCTGAAACCTATGAGAACGAGACTAAGTTGGCCCTTGTTGGGAACTGG
GGCACAACGGGCCTCACCTACCCCAAGTTTTCTGACGTCACGGGCAAGATCAAGCTACCC
AAGGACAGCTTCCGCCCCTCGGCCGGCTGGACCTGGGCTGGAGATTGGTTCGTGTGTCCG
GAGAAGACTCTGCTCCATGACATGGACGCCGGTCACCTGAGCTTCGTGGAAGAGGTGTTT
GAGAACCAGACCCGGCTTCCCGGAGGCCAGTGGATCTACATGAGTGACAACTACACCGAT
GTGAACGGGGAGAAGGTGCTTCCCAAGGATGACATTGAGTGCCCACTGGGCTGGAAGTGG
GAAGATGAGGAATGGTCCACAGACCTCAACCGGGCTGTCGATGAGCAAGGCTGGGAGTAT
AGCATCACCATCCCCCCGGAGCGGAAGCCGAAGCACTGGGTCCCTGCTGAGAAGATGTAC
TACACACACCGACGGCGGCGCTGGGTGCGCCTGCGCAGGAGGGATCTCAGCCAAATGGAA
GCACTGAAAAGGCACAGGCAGGCGGAGGCGGAGGGCGAGGGCTGGGAGTACGCCTCTCTT
TTTGGCTGGAAGTTCCACCTCGAGTACCGCAAGACAGATGCCTTCCGCCGCCGCCGCTGG
CGCCGTCGCATGGAGCCACTGGAGAAGACGGGGCCTGCAGCTGTGTTTGCCCTTGAGGGG
GCCCTGGGCGGCGTGATGGATGACAAGAGTGAAGATTCCATGTCCGTCTCCACCTTGAGC
TTCGGTGTGAACAGACCCACGATTTCCTGCATATTCGACTATGGGAACCGCTACCATCTA
CGCTGCTACATGTACCAGGCCCGGGACCTGGCTGCGATGGACAAGGACTCTTTTTCTGAT
CCCTATGCCATCGTCTCCTTCCTGCACCAGAGCCAGAAGACGGTGGTGGTGAAGAACACC
CTTAACCCCACCTGGGACCAGACGCTCATCTTCTACGAGATCGAGATCTTTGGCGAGCCG
GCCACAGTTGCTGAGCAACCGCCCAGCATTGTGGTGGAGCTGTACGACCATGACACTTAT
GGTGCAGACGAGTTTATGGGTCGCTGCATCTGTCAACCGAGTCTGGAACGGATGCCACGG
CTGGCCTGGTTCCCACTGACGAGGGGCAGCCAGCCGTCGGGGGAGCTGCTGGCCTCTTTT
GAGCTCATCCAGAGAGAGAAGCCGGCCATCCACCATATTCCTGGTTTTGAGGTGCAGGAG
ACATCAAGGATCCTGGATGAGTCTGAGGACACAGACCTGCCCTACCCACCACCCCAGAGG
GAGGCCAACATCTACATGGTTCCTCAGAACATCAAGCCAGCGCTCCAGCGTACCGCCATC
GAGATCCTGGCATGGGGCCTGCGGAACATGAAGAGTTACCAGCTGGCCAACATCTCCTCC
CCCAGCCTCGTGGTAGAGTGTGGGGGCCAGACGGTGCAGTCCTGTGTCATCAGGAACCTC
CGGAAGAACCCCAACTTTGACATCTGCACCCTCTTCATGGAAGTGATGCTGCCCAGGGAG
GAGCTCTACTGCCCCCCCATCACCGTCAAGGTCATCGATAACCGCCAGTTTGGCCGCCGG
CCTGTGGTGGGCCAGTGTACCATCCGCTCCCTGGAGAGCTTCCTGTGTGACCCCTACTCG
GCGGAGAGTCCATCCCCACAGGGTGGCCCAGACGATGTGAGCCTACTCAGTCCTGGGGAA
GACGTGCTCATCGACATTGATGACAAGGAGCCCCTCATCCCCATCCAGGAGGAAGAGTTC
ATCGATTGGTGGAGCAAATTCTTTGCCTCCATAGGGGAGAGGGAAAAGTGCGGCTCCTAC
CTGGAGAAGGATTTTGACACCCTGAAGGTCTATGACACACAGCTGGAGAATGTGGAGGCC
TTTGAGGGCCTGTCTGACTTTTGTAACACCTTCAAGCTGTACCGGGGCAAGACGCAGGAG
GAGACAGAAGATCCATCTGTGATTGGTGAATTTAAGGGCCTCTTCAAAATTTATCCCCTC
CCAGAAGACCCAGCCATCCCCATGCCCCCAAGACAGTTCCACCAGCTGGCCGCCCAGGGA
CCCCAGGAGTGCTTGGTCCGTATCTACATTGTCCGAGCATTTGGCCTGCAGCCCAAGGAC
CCCAATGGAAAGTGTGATCCTTACATCAAGATCTCCATAGGGAAGAAATCAGTGAGTGAC
CAGGATAACTACATCCCCTGCACGCTGGAGCCCGTATTTGGAAAGATGTTCGAGCTGACC
TGCACTCTGCCTCTGGAGAAGGACCTAAAGATCACTCTCTATGACTATGACCTCCTCTCC
AAGGACGAAAAGATCGGTGAGACGGTCGTCGACCTGGAGAACAGGCTGCTGTCCAAGTTT
GGGGCTCGCTGTGGACTCCCACAGACCTACTGTGTCTCTGGACCGAACCAGTGGCGGGAC
CAGCTCCGCCCCTCCCAGCTCCTCCACCTCTTCTGCCAGCAGCATAGAGTCAAGGCACCT
GTGTACCGGACAGACCGTGTAATGTTTCAGGATAAAGAATATTCCATTGAAGAGATAGAG
GCTGGCAGGATCCCAAACCCACACCTGGGCCCAGTGGAGGAGCGTCTGGCTCTGCATGTG
CTTCAGCAGCAGGGCCTGGTCCCGGAGCACGTGGAGTCACGGCCCCTCTACAGCCCCCTG
CAGCCAGACATCGAGCAGGGGAAGCTGCAGATGTGGGTCGACCTATTTCCGAAGGCCCTG
GGGCGGCCTGGACCTCCCTTCAACATCACCCCACGGAGAGCCAGAAGGTTTTTCCTGCGT
TGTATTATCTGGAATACCAGAGATGTGATCCTGGATGACCTGAGCCTCACGGGGGAGAAG
ATGAGCGACATTTATGTGAAAGGTTGGATGATTGGCTTTGAAGAACACAAGCAAAAGACA
GACGTGCATTATCGTTCCCTGGGAGGTGAAGGCAACTTCAACTGGAGGTTCATTTTCCCC
TTCGACTACCTGCCAGCTGAGCAAGTCTGTACCATTGCCAAGAAGGATGCCTTCTGGAGG
CTGGACAAGACTGAGAGCAAAATCCCAGCACGAGTGGTGTTCCAGATCTGGGACAATGAC
AAGTTCTCCTTTGATGATTTTCTGGGCTCCCTGCAGCTCGATCTCAACCGCATGCCCAAG
CCAGCCAAGACAGCCAAGAAGTGCTCCTTGGACCAGCTGGATGATGCTTTCCACCCAGAA
TGGTTTGTGTCCCTTTTTGAGCAGAAAACAGTGAAGGGCTGGTGGCCCTGTGTAGCAGAA
GAGGGTGAGAAGAAAATACTGGCGGGCAAGCTGGAAATGACCTTGGAGATTGTAGCAGAG
AGTGAGCATGAGGAGCGGCCTGCTGGCCAGGGCCGGGATGAGCCCAACATGAACCCTAAG
CTTGAGGACCCAAGGCGCCCCGACACCTCCTTCCTGTGGTTTACCTCCCCATACAAGACC
ATGAAGTTCATCCTGTGGCGGCGTTTCCGGTGGGCCATCATCCTCTTCATCATCCTCTTC
ATCCTGCTGCTGTTCCTGGCCATCTTCATCTACGCCTTCCCGAACTATGCTGCCATGAAG
CTGGTGAAGCCCTTCAGC
Restriction Sites Please inquire     
ACCN NM_001130976
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001130976.1, NP_001124448.1
RefSeq Size 6848 bp
RefSeq ORF 6201 bp
Locus ID 8291
UniProt ID O75923
Protein Families Transmembrane
Gene Summary The protein encoded by this gene belongs to the ferlin family and is a skeletal muscle protein found associated with the sarcolemma. It is involved in muscle contraction and contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. In addition, the protein encoded by this gene binds caveolin-3, a skeletal muscle membrane protein which is important in the formation of caveolae. Specific mutations in this gene have been shown to cause autosomal recessive limb girdle muscular dystrophy type 2B (LGMD2B) as well as Miyoshi myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2008]
Transcript Variant: This variant (9) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (9) with an alternate N-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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