CERKL (NM_001160277) Human Untagged Clone

CAT#: SC327056

CERKL (untagged)-Human ceramide kinase-like (CERKL) transcript variant 7



  "NM_001160277" in other vectors (4)

CNY 8,270.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-CERKL Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms RP26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC327056 representing NM_001160277.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCCTGGAGGAGGCGCAGGAACCGGGTGAGTGCCCTGGAGGGCGGCCGGGAGGAAGAGGCGCCCCCG
GAGGCTGCCGCTGTGCCTCCGGCGCTGTTAACGTCCCCGCAGCAGACGGAGGCGGCGGCCGAGCGGATT
CTGCTCCGGGGCATCTTCGAGATCGGGAGGGACAGTTGTGACGTGGTGCTGAGCGAGCGAGCACTGCGG
TGGCGGCCCATTCAGCCCGAGCGCCCGGCGGGTGATTCTAAGTATGACTTGCTATGTAAAGAAGAATTT
ATTGAACTCAAAGACATATTCTCTGTGAAACTGAAACGGCGTTGTTCTGTTAAACAGCAGAGAAGTGGT
ACTTTATTAGGTATCACACTCTTCATCTGCTTGAAAAAGGAACAAAATAAACTAAAGAATTCTACACTT
GATCTTATTAATTTAAGTGAAGACCACTGTGACATATGGTTTAGACAGTTCAAGAAAATATTGGCAGTA
ATGGAATATGAAGGGCACGCTCTGTCACTGCTTAAGGAATGTGAACTCCAGGGATTTGATGGTGGGCAT
CGGAAACCATTGTTCGCAATACATTGGAGTGTACAGAGATTGTTCACTGGCATGCAAACGTTAGAACCA
AGTGTTGTCTGTGTTGGTGGAGATGGATCTGCTAGCGAAGTAGCCCATGCTTTGCTTCTGAGAGCTCAG
AAGAATGCTGGGATGGAAACAGACCGAATCCTGACTCCTGTCAGAGCACAGCTTCCACTTGGCTTAATA
CCAGCAGGATCTACCAATGTATTGGCACATTCTCTTCATGGAGTTCCTCATGTGATAACTGCAACATTG
CACATTATAATGGGGCATGTACAGCTGGTCGACGTCTGCACCTTCAGCACCGCTGGCAAGCTTCTTCGC
TTTGGGTTCTCAGCCATGTTTGGCTTTGGTGGAAGAACTTTGGCTCTGGCAGAAAAATATCGATGGATG
TCCCCTAACCAACGGAGAGATTTTGCTGTTGTTAAGGCACTGGCAAAACTTAAGGCAGAAGACTGTGAA
ATATCATTTTTACCATTTAACAGCTCTGATGATGTGCAAGAAAGGAGGGCACAGGGATCTCCCAAATCT
GACTGTAATGATCAATGGCAAATGATCCAGGGTCAGTTCTTGAATGTCAGCATTATGGCAATTCCTTGC
CTGTGTTCAGTGGCACCTAGAGGCTTGGCACCTAATACCAGATTAAATAATGGAAGTATGGCTCTTATA
ATTGCCCGAAACACTTCTCGGCCAGAATTTATAAAACACCTGAAAAGATATGCCAGTGTAAAAAATCAG
TTCAATTTTCCATTTGTTGAGACTTACACTGTTGAGGAAGTAAAAGTTCATCCAAGGAATAATACTGGT
GGATATAATCCAGAGGAGGAGGAGGATGAAACTGCTTCAGAAAATTGTTTCCCTTGGAATGTAGATGGT
GACTTAATGGAAGTTGCATCAGAGGTCCATATTAGATTGCATCCAAGACTTATCAGTCTTTATGGAGGA
AGCATGGAAGAAATGATTCCAAAGTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001160277
Insert Size 1545 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001160277.1
RefSeq Size 3172 bp
RefSeq ORF 1545 bp
Locus ID 375298
UniProt ID Q49MI3
Protein Families Druggable Genome
MW 57.6 kDa
Gene Summary This gene was initially identified as a locus (RP26) associated with an autosomal recessive form of retinitis pigmentosa (arRP) disease. This gene encodes a protein with ceramide kinase-like domains, however, the protein does not phosphorylate ceramide and its target substrate is currently unknown. This protein may be a negative regulator of apoptosis in photoreceptor cells. Mutations in this gene cause a form of retinitis pigmentosa characterized by autosomal recessive cone and rod dystrophy (arCRD). Alternative splicing of this gene results in multiple transcript variants encoding different isoforms and non-coding transcripts.[provided by RefSeq, May 2010]
Transcript Variant: This variant (7) lacks one alternate in-frame exon in the central coding region, compared to variant 2. The resulting isoform (7) lacks an internal segment, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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