TOPBP1 (NM_007027) Human Untagged Clone

CAT#: SC328077

TOPBP1 (untagged)-Human topoisomerase (DNA) II binding protein 1 (TOPBP1)



  "NM_007027" in other vectors (7)

CNY 10,450.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TOPBP1 mouse monoclonal antibody,clone OTI5C12
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms Dpb11; TOP2BP1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_007027, the custom clone sequence may differ by one or more nucleotides
ATGTCCAGAAATGACAAAGAACCGTTTTTTGTGAAGTTTTTAAAGTCTTCAGACAATTCC
AAATGTTTTTTTAAAGCTCTCGAGTCCATAAAAGAATTCCAATCAGAAGAATATCTTCAG
ATTATTACAGAAGAAGAGGCATTGAAGATAAAGGAGAATGATAGATCACTTTATATCTGT
GACCCTTTTAGTGGCGTTGTCTTTGATCACCTCAAAAAGCTTGGCTGCAGAATTGTTGGT
CCTCAAGTAGTCATATTTTGTATGCACCACCAGCGATGTGTCCCAAGAGCCGAACATCCA
GTTTATAATATGGTTATGTCTGATGTAACCATATCTTGTACAAGTCTGGAAAAAGAAAAA
AGGGAAGAAGTTCATAAATATGTACAAATGATGGGCGGACGAGTATACAGAGACCTTAAT
GTATCAGTAACTCACCTTATTGCAGGAGAAGTTGGTAGCAAAAAATATTTAGTTGCTGCA
AACCTGAAGAAACCTATTTTGCTTCCCTCTTGGATAAAAACACTTTGGGAGAAGTCACAA
GAGAAAAAAATAACTAGATATACTGATATAAACATGGAAGATTTCAAGTGTCCTATTTTT
CTTGGTTGCATAATCTGTGTGACTGGCTTATGTGGCTTAGACAGGAAAGAAGTTCAGCAA
CTCACAGTTAAGCATGGAGGTCAATACATGGGACAATTGAAAATGAATGAATGTACACAC
CTCATTGTGCAAGAACCAAAAGGTCAGAAGTATGAGTGTGCCAAGAGATGGAATGTACAC
TGTGTGACCACACAGTGGTTTTTTGACAGTATTGAGAAAGGTTTTTGTCAGGATGAATCC
ATATACAAGACAGAACCTAGACCAGAAGCAAAGACTATGCCCAATTCTTCAACTCCTACC
AGCCAGATCAACACAATTGATAGTCGTACTCTTTCAGATGTCAGCAATATTTCCAACATA
AATGCAAGTTGCGTAAGTGAATCAATATGTAATTCACTTAACAGCAAACTGGAGCCTACA
CTTGAAAATCTAGAAAATCTGGATGTCAGTGCATTTCAAGCACCTGAAGATTTATTAGAT
GGTTGTCGGATATATCTTTGCGGTTTTAGTGGCAGAAAGCTAGATAAACTGAGAAGACTT
ATTAACAGTGGAGGTGGAGTTCGTTTTAACCAGCTAAATGAAGATGTAACTCATGTTATT
GTGGGAGATTATGATGATGAATTGAAGCAGTTTTGGAATAAATCAGCCCACAGGCCTCAT
GTAGTGGGAGCAAAGTGGTTGCTAGAGTGTTTCAGTAAAGGTTATATGCTTTCTGAAGAA
CCATATATCCATGCTAATTACCAGCCAGTGGAAATTCCAGTTTCACATAAGCCTGAAAGT
AAAGCAGCTCTTTTAAAAAAGAAGAACAGCAGCTTCTCTAAGAAAGACTTTGCTCCTAGT
GAAAAGCATGAGCAAGCTGATGAAGATCTGCTCTCTCAATATGAAAATGGTAGCTCCACA
GTAGTTGAGGCTAAGACGTCTGAAGCCAGGCCCTTTAATGATTCTACTCATGCTGAGCCC
TTGAATGATTCTACTCACATTTCTTTGCAAGAAGAAAACCAGTCTTCTGTCAGTCATTGT
GTCCCTGATGTTTCTACAATTACTGAAGAAGGCTTATTTAGCCAAAAGAGTTTCCTTGTT
TTGGGTTTTAGTAATGAAAATGAATCTAACATCGCAAACATCATAAAAGAAAATGCTGGG
AAAATCATGTCCCTTCTGAGCAGAACTGTTGCGGATTATGCTGTGGTTCCTCTGCTGGGG
TGTGAAGTGGAAGCCACTGTGGGAGAAGTTGTTACAAATACATGGCTGGTTACTTGCATA
GACTATCAGACTTTGTTTGATCCAAAGTCGAATCCTCTCTTCACACCAGTTCCAGTAATG
ACAGGAATGACTCCTTTAGAGGATTGTGTTATTTCATTTAGCCAGTGTGCTGGAGCAGAA
AAAGAGTCTTTAACATTCCTAGCAAACCTCCTTGGAGCAAGTGTTCAAGAATACTTTGTT
CGCAAATCCAATGCAAAGAAAGGCATGTTTGCCAGTACTCATCTTATACTGAAAGAACGT
GGTGGCTCTAAATATGAAGCTGCAAAGAAGTGGAATTTACCTGCCGTTACTATAGCTTGG
CTGTTGGAGACTGCTAGAACGGGAAAGAGAGCAGACGAAAGCCATTTTCTGATTGAAAAT
TCAACTAAAGAAGAACGAAGTTTGGAAACAGAAATAACAAATGGAATCAATCTAAATTCA
GATACTGCAGAGCATCCTGGCACACGCCTGCAAACTCACAGAAAAACCGTCGTTACACCT
TTAGATATGAACCGCTTTCAGAGTAAAGCTTTCCGTGCTGTGGTCTCACAACATGCCAGA
CAGGTCGCAGCCTCCCCAGCAGTAGGACAACCACTTCAGAAGGAGCCCTCGTTACACCTG
GATACACCATCAAAATTCCTGTCCAAGGACAAACTCTTCAAGCCTTCCTTTGATGTGAAG
GATGCACTTGCAGCCTTGGAAACTCCAGGACGTCCCAGCCAACAGAAAAGGAAACCGAGT
ACGCCACTCTCAGAAGTTATTGTCAAAAACTTGCAACTTGCTTTGGCAAATAGCTCTCGA
AATGCTGTCGCTCTTTCTGCCAGCCCTCAACTGAAAGAGGCCCAGTCAGAGAAGGAAGAA
GCCCCAAAGCCACTTCACAAAGTAGTGGTATGTGTTAGTAAAAAACTCAGTAAGAAGCAG
AGTGAACTAAATGGGATCGCAGCCTCTCTAGGAGCAGATTACAGGTGGAGTTTTGATGAA
ACAGTGACTCATTTCATCTATCAAGGGCGGCCAAATGACACTAATCGGGAGTATAAATCT
GTAAAAGAAAGAGGAGTACACATTGTTTCCGAGCACTGGCTTTTAGATTGTGCCCAAGAG
TGTAAACATCTTCCTGAATCTCTTTATCCACATACTTATAATCCCAAAATGAGCTTGGAT
ATCAGCGCAGTGCAAGATGGCCGGCTCTGTAATAGTCGACTACTCTCAGCTGTGTCTTCA
ACAAAGGATGATGAGCCAGATCCTTTGATTTTAGAAGAAAATGATGTAGACAATATGGCC
ACCAATAATAAAGAGTCAGCACCATCAAATGGAAGTGGAAAGAATGACTCTAAAGGAGTT
CTGACACAGACCTTAGAGATGAGAGAGAACTTTCAGAAGCAGTTACAGGAGATAATGTCT
GCAACATCAATAGTGAAACCCCAAGGGCAGAGGACTTCCCTTTCAAGAAGTGGTTGTAAC
AGCGCATCTTCAACCCCTGACAGCACTCGCTCTGCTCGCAGTGGACGAAGTAGAGTCCTA
GAGGCACTGAGGCAGTCTCGTCAGACAGTACCTGATGTCAACACAGAGCCTTCCCAAAAT
GAACAGATCATTTGGGATGACCCTACAGCAAGGGAGGAGAGAGCAAGGCTTGCCAGCAAT
TTGCAGTGGCCTAGTTGTCCCACACAATACTCTGAGCTTCAGGTTGACATTCAAAACTTG
GAGGATTCTCCTTTTCAAAAGCCTTTACATGATTCAGAAATTGCTAAACAGGCTGTCTGT
GATCCTGGAAACATACGTGTGACTGAAGCTCCCAAACACCCAATCTCTGAAGAACTGGAA
ACTCCCATAAAAGACAGCCACCTGATCCCTACGCCTCAAGCCCCCAGTATTGCCTTTCCA
CTCGCCAACCCCCCTGTGGCTCCGCACCCTAGAGAAAAGATTATAACGATAGAGGAGACT
CATGAAGAATTAAAAAAACAGTACATATTTCAGTTATCATCTCTGAATCCTCAAGAACGT
ATTGACTATTGTCATCTGATTGAGAAACTAGGTGGATTGGTGATAGAAAAGCAGTGCTTT
GATCCCACCTGTACACACATTGTTGTGGGACATCCACTTCGAAACGAGAAGTATTTAGCC
TCAGTGGCAGCTGGGAAGTGGGTGCTTCATCGCTCCTACCTTGAAGCCTGCAGGACTGCT
GGACACTTCGTGCAGGAAGAAGACTATGAATGGGGAAGTAGTTCCATACTTGATGTTCTG
ACTGGAATCAATGTACAGCAACGAAGACTAGCACTTGCAGCAATGAGATGGAGAAAAAAA
ATCCAGCAAAGACAAGAATCTGGCATTGTTGAGGGAGCATTTAGTGGGTGGAAGGTTATT
TTACATGTGGATCAGTCTCGAGAAGCAGGCTTCAAACGCCTTCTTCAGTCAGGAGGAGCA
AAGGTGCTACCTGGTCATTCTGTACCTTTATTTAAAGAGGCCACACATCTTTTTTCTGAC
TTGAATAAACTGAAACCAGATGACTCAGGAGTTAATATAGCAGAAGCTGCTGCCCAGAAC
GTGTACTGCTTGAGAACAGAATACATTGCTGATTATCTCATGCAGGAATCACCTCCTCAT
GTAGAAAATTACTGTCTACCAGAAGCTATTTCATTTATTCAGAATAATAAGGAACTTGGG
ACTGGATTATCACAAAAGAGGAAAGCTCCTACAGAAAAAAATAAAATCAAACGACCTAGA
GTACAC
Restriction Sites Please inquire     
ACCN NM_007027
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007027.3, NP_008958.2
RefSeq Size 5378 bp
RefSeq ORF 4569 bp
Locus ID 11073
UniProt ID Q92547
Domains BRCT
Gene Summary This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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