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GRAMD1C (NM_001172105) Human Untagged Clone
CAT＃: SC328753
GRAMD1C (untagged)-Human GRAM domain containing 1C (GRAMD1C) transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328753 representing NM_001172105.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAAACTTGTCACTGTCGATTGAGGATGTGCAGCCAAGAAGTCCAGGAAGAAGCAGCTTGGATGAC TCTGGGGAGAGAGATGAAAAATTATCCAAGTCAATCAGTTTTACCAGTGAATCAATTAGTCGGGTTTCA GAAACAGAGTCATTCGATGGAAATTCATCAAAAGGAGGATTAGGCAAAGAGGAGTCCCAAAATGAGAAA CAGACCAAAAAGAGTCTCTTACCAACTTTGGAAAAGAAGTTAACTAGAGTGCCATCAAAGTCACTGGAC TTGAATAAAAATGAATATCTTTCTCTGGACAAAAGCAGCACTTCAGATTCTGTTGATGAAGAAAATGTT CCTGAGAAAGATCTTCATGGAAGACTTTTTATCAACCGTATTTTTCATATCAGTGCTGACAGAATGTTT GAATTGCTCTTTACCAGTTCACGCTTTATGCAGAAATTTGCCAGTTCTAGAAATATAATAGATGTAGTA TCTACCCCTTGGACTGCAGAACTTGGAGGTGATCAGCTGAGAACGATGACCTACACTATAGTCCTTAAT AGTCCACTTACTGGAAAATGCACTGCTGCCACTGAAAAGCAGACACTGTATAAAGAAAGTCGGGAAGCA CGATTTTATTTGGTAGATTCAGAAGTACTGACACATGATGTCCCCTACCATGATTACTTCTATACCGTG AACAGATACTGTATCATCCGATCTTCAAAACAGAAATGCAGGCTAAGAGTTTCCACAGATTTGAAATAC AGAAAACAGCCATGGGGCCTTGTCAAATCTTTAATTGAAAAGAATTCCTGGAGTTCTTTGGAGGACTAT TTCAAACAGCTTGAATCAGATTTGTTAATTGAAGAATCTGTATTAAATCAGGCCATTGAAGACCCTGGA AAACTTACTGGCCTACGAAGGAGAAGGCGAACCTTCAACCGAACAGCAGAAACAGTTCCTAAACTTTCC TCTCAGCATTCCTCTGGAGATGTGGGCTTAGGTGCCAAAGGGGATATTACAGGAAAGAAAAAGGAAATG GAAAACTATAACGTCACTCTTATTGTGGTAATGAGTATTTTTGTGTTGTTATTAGTTTTGTTGAATGTG ACACTGTTTCTGAAGCTGTCAAAGATAGAACATGCTGCTCAGTCCTTTTACCGTCTCCGCCTCCAAGAA GAGAAATCTTTAAATTTAGCCTCTGATATGGTGTCAAGAGCAGAAACTATTCAGAAGAATAAAGATCAG GCCCATCGTTTAAAGGGAGTGCTCCGAGACTCCATAGTGATGCTTGAACAGCTGAAGAGCTCACTCATT ATGCTTCAGAAAACGTTTGATCTACTAAATAAGAATAAGACTGGCATGGCTGTTGAAAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001172105 |
| Insert Size | 1374 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001172105.1 |
| RefSeq Size | 3497 bp |
| RefSeq ORF | 1374 bp |
| Locus ID | 54762 |
| UniProt ID | Q8IYS0 |
| Protein Families | Transmembrane |
| MW | 52 kDa |
| Gene Summary | Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (By similarity). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (By similarity). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (By similarity). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (By similarity). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and uses a downstream start codon, compared to variant 1. The resulting isoform (2) has a substantially shorter N-terminus, compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230115 | GRAMD1C (Myc-DDK-tagged)-Human GRAM domain containing 1C (GRAMD1C), transcript variant 2 |
CNY 3,656.00 |
|
| RC230115L3 | Lenti-ORF clone of GRAMD1C (Myc-DDK-tagged)-Human GRAM domain containing 1C (GRAMD1C), transcript variant 2 |
CNY 5,890.00 |
|
| RC230115L4 | Lenti-ORF clone of GRAMD1C (mGFP-tagged)-Human GRAM domain containing 1C (GRAMD1C), transcript variant 2 |
CNY 5,890.00 |
|
| RG230115 | GRAMD1C (tGFP-tagged) - Human GRAM domain containing 1C (GRAMD1C), transcript variant 2 |
CNY 4,370.00 |
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