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DNA Polymerase lambda (POLL) (NM_001174085) Human Untagged Clone
CAT＃: SC328795
POLL (untagged)-Human polymerase (DNA directed) lambda (POLL) transcript variant 3
CNY 7,790.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BETAN; POLKAPPA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328795 representing NM_001174085.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATCCCAGGGGTATCTTGAAGGCATTTCCCAAGCGGCAGAAAATTCATGCTGATGCATCATCAAAA GTACTTGCAAAGATTCCTAGGAGGGAAGAGGGAGAAGAAGCAGAAGAGTGGCTGAGCTCCCTTCGGGCC CATGTTGTGCGCACTGGCATTGGACGAGCCCGGGCAGAACTCTTTGAGAAGCAGATTGTTCAGCATGGC GGCCAGCTATGCCCTGCCCAGGGCCCAGGTGTCACTCACATTGTGGTGGATGAAGGCATGGACTATGAG CGAGCCCTCCGCCTTCTCAGACTACCCCAGCTGCCCCCGGGTGCTCAGCTGGTGAAGTCAGCCTGGCTG AGCTTGTGCCTTCAGGAGAGGAGGCTGGTGGATGTAGCTGGATTCAGCATCTTCATCCCCAGTAGGTAC TTGGACCATCCACAGCCCAGCAAGGCAGAGCAGGATGCTTCTATTCCTCCTGGCACCCATGAGGCCCTG CTTCAGACAGCCCTTTCTCCTCCTCCTCCTCCCACCAGGCCTGTGTCTCCTCCCCAAAAGGCAAAAGAG GCACCAAACACCCAAGCCCAGCCCATCTCTGATGATGAAGCCAGTGATGGGGAAGAAACCCAGGAGGCC TGCAGTATCCCTGGGATTGGGAAGCGGATGGCTGAGAAAATCATAGAGATCCTGGAGAGCGGGCATTTG CGGAAGCTGGACCATATCAGTGAGAGCGTGCCTGTCTTGGAGCTCTTCTCCAACATCTGGGGAGCTGGG ACCAAGACTGCCCAGATGTGGTACCAACAGGGCTTCCGAAGTCTGGAAGACATCCGCAGCCAGGCCTCC CTGACAACCCAGCAGGCCATCGGCCTGAAGCATTACAGTGACTTCCTGGAACGTATGCCCAGGGAGGAG GCTACAGAGATTGAGCAGACAGTCCAGAAAGCAGCCCAGGCCTTTAACTCTGGGCTGCTGTGTGTGGCA TGTGGTTCATACCGACGGGGAAAGGCGACCTGTGGTGATGTCGACGTGCTCATCACTCACCCAGATGGC CGGTCCCACCGGGGTATCTTCAGCCGCCTCCTTGACAGTCTTCGGCAGGAAGGGTTCCTCACAGATGAC TTGGTGAGCCAAGAGGAGAATGGTCAGCAACAGAAGTACTTGGGGGTGTGCCGGCTCCCAGGGCCAGGG CGGCGGCACCGGCGCCTGGACATCATCGTGGTGCCCTATAGCGAGTTTGCCTGTGCCCTGCTCTACTTC ACCGGCTCTGCACACTTCAACCGCTCCATGCGAGCCCTGGCCAAAACCAAGGGCATGAGTCTGTCAGAA CATGCCCTCAGCACTGCTGTGGTCCGGAACACCCATGGCTGCAAGGTGGGGCCTGGCCGAGTGCTGCCC ACTCCCACTGAGAAGGATGTCTTCAGGCTCTTAGGCCTCCCCTACCGAGAACCTGCTGAGCGGGACTGG TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001174085 |
| Insert Size | 1452 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001174085.1 |
| RefSeq Size | 2465 bp |
| RefSeq ORF | 1452 bp |
| Locus ID | 27343 |
| UniProt ID | Q9UGP5 |
| Protein Families | Druggable Genome |
| Protein Pathways | Base excision repair, Non-homologous end-joining |
| MW | 53.5 kDa |
| Gene Summary | This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice site in the middle portion of the coding region, compared to variant 1. This results in a shorter protein (isoform b, also known as lambda2), compared to isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230157 | POLL (Myc-DDK-tagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 3 |
CNY 3,656.00 |
|
| RC230157L3 | Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC230157L4 | Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG230157 | POLL (tGFP-tagged) - Human polymerase (DNA directed), lambda (POLL), transcript variant 3 |
CNY 4,370.00 |
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