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MAN1 (LEMD3) (NM_001167614) Human Untagged Clone
CAT＃: SC329219
LEMD3 (untagged)-Human LEM domain containing 3 (LEMD3) transcript variant 2
CNY 17,480.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MAN1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC329219 representing NM_001167614.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCGGCAGCAGCTTCGGCGCCTCAGCAGCTCTCGGATGAGGAGCTTTTCTCTCAGCTCCGCCGT TACGGCCTGTCTCCCGGACCAGTGACGGAGAGCACCCGCCCGGTCTACCTCAAGAAGCTGAAGAAGCTT CGAGAGGAAGAGCAGCAACAGCACCGGTCAGGGGGCCGCGGCAACAAGACGCGGAACAGTAATAACAAT AACACGGCAGCCGCCACGGTCGCAGCCGCGGGACCAGCGGCGGCGGCGGCCGCGGGGATGGGGGTCCGG CCGGTCTCGGGCGACCTCTCCTACTTACGGACTCCTGGGGGCCTGTGCCGAATCTCGGCCTCTGGCCCA GAGAGCCTCCTGGGAGGGCCCGGGGGCGCCTCCGCCGCCCCCGCGGCTGGCAGCAAAGTGCTGCTGGGC TTCAGCTCGGACGAGTCGGACGTGGAGGCCAGTCCCCGGGACCAGGCCGGCGGCGGCGGGAGGAAAGAC CGGGCTTCGCTCCAGTACCGCGGGCTCAAAGCGCCGCCGGCGCCCCTGGCCGCCAGCGAGGTGACTAAC AGCAACTCTGCAGAGCGAAGGAAGCCCCACTCGTGGTGGGGGGCCAGGAGGCCGGCGGGCCCCGAGCTG CAGACCCCGCCGGGGAAAGATGGAGCAGTGGAGGACGAGGAAGGGGAGGGAGAGGACGGTGAGGAGAGG GACCCGGAGACCGAGGAGCCGCTCTGGGCGAGCCGGACCGTGAATGGCAGCCGGCTTGTCCCCTACAGC TGCCGGGAAAACTATTCGGACTCAGAGGAAGAGGACGACGACGACGTGGCCTCCAGCAGACAGGTATTA AAGGACGACTCCCTTTCCCGGCATCGGCCCAGACGAACCCATAGTAAGCCTCTCCCCCCGCTGACTGCT AAATCGGCCGGCGGCAGGCTGGAGACTTCAGTTCAGGGAGGGGGAGGACTCGCGATGAATGACAGGGCG GCGGCTGCCGGGAGTCTAGACAGGAGCCGAAACCTCGAAGAGGCGGCGGCCGCGGAGCAGGGAGGAGGG TGTGATCAAGTGGACTCCAGCCCCGTTCCTAGATACCGTGTTAACGCTAAGAAACTGACCCCTCTCCTG CCCCCGCCACTTACTGACATGGACTCAACCTTGGATTCGTCAACAGGCTCCCTTCTGAAAACCAATAAT CATATTGGCGGTGGGGCCTTCAGTGTGGACTCCCCCAGGATTTATTCTAACAGTCTCCCTCCCAGTGCG GCGGTGGCCGCCTCTAGTTCACTCAGGATCAATCACGCCAATCATACGGGCTCCAATCATACCTACCTG AAAAACACATACAACAAACCGAAGCTTTCCGAACCCGAAGAGGAACTTCTCCAGCAATTTAAACGGGAG GAGGTGTCCCCAACAGGGAGTTTCAGTGCCCACTACTTGTCGATGTTTCTCTTAACTGCTGCCTGCTTA TTTTTCCTAATACTGGGACTGACTTACCTAGGAATGAGAGGGACAGGAGTATCTGAGGATGGAGAACTC AGCAAAAACCCCTTTGGTGAAACATTTGGAAAAATACAAGAAAGTGAAAAAACTCTTATGATGAACACA TTATATAAGCTTCATGATCGATTGGCACAGCTTGCAGGAGATCATGAATGTGGCAGTTCTAGTCAAAGA ACGCTTTCTGTTCAAGAGGCAGCTGCGTATTTAAAAGATTTAGGTCCTGAATATGAAGGTATATTTAAC ACTTCATTGCAGTGGATCTTAGAAAATGGAAAAGATGTTGGAATAAGGTGTGTTGGTTTTGGCCCTGAG GAAGAATTGACAAATATAACTGATGTGCAGTTTTTACAGTCCACAAGACCACTGATGTCTTTTTGGTGT CGTTTTCGACGTGCTTTTGTTACTGTAACTCACAGATTATTGTTGTTATGCTTAGGTGTAGTGATGGTT TGTGTCGTTCTGCGTTACATGAAATATCGATGGACAAAAGAAGAGGAGGAAACAAGGCAGATGTATGAT ATGGTGGTAAAGATTATAGATGTTTTACGAAGTCATAATGAAGCCTGCCAGGAAAACAAAGATTTACAA CCTTACATGCCTATTCCACATGTACGCGATTCCTTAATACAGCCTCATGACAGGAAAAAAATGAAGAAA GTCTGGGATAGAGCTGTTGACTTCCTTGCTGCTAATGAGTCTAGAGTTCGCACGGAAACACGAAGAATA GGTGGTGCAGATTTTCTGGTTTGGCGGTGGATCCAGCCTTCTGCATCCTGTGACAAAATATTAGTTATA CCTTCTAAAGTATGGCAAGGTCAAGCATTTCATTTAGATAGAAGAAATTCACCACCAAATAGTTTGACA CCGTGTCTAAAGATTCGGAATATGTTTGATCCCGTTATGGAAATAGGGGATCAGTGGCATTTGGCAATT CAAGAAGCAATTTTAGAAAAATGCAGTGATAATGATGGCATTGTTCACATTGCAGTAGACAAAAATTCA CGTGAGGGTTGTGTATATGTTAAATGTCTGTCTCCAGAATATGCTGGAAAGGCTTTTAAAGCATTGCAT GGCTCTTGGTTTGATGGGAAATTGGTTACAGTAAAATATTTACGACTAGATAGATACCACCATCGCTTT CCCCAGGCTCTCACTTCCAACACTCCATTGAAGCCATCAAATAAACATATGAACTCCATGTCTCATCTT CGTCTTCGGACTGGCCTAACCAATTCTCAAGGAAGTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001167614 |
| Insert Size | 2733 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001167614.1 |
| RefSeq Size | 4795 bp |
| RefSeq ORF | 2733 bp |
| Locus ID | 23592 |
| UniProt ID | Q9Y2U8 |
| Protein Families | Transmembrane |
| MW | 99.9 kDa |
| Gene Summary | This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.[provided by RefSeq, Nov 2009] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230581 | LEMD3 (Myc-DDK-tagged)-Human LEM domain containing 3 (LEMD3), transcript variant 2 |
CNY 6,664.00 |
|
| RC230581L3 | Lenti ORF clone of Human LEM domain containing 3 (LEMD3), transcript variant 2, Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC230581L4 | Lenti ORF clone of Human LEM domain containing 3 (LEMD3), transcript variant 2, mGFP tagged |
CNY 11,500.00 |
|
| RG230581 | LEMD3 (tGFP-tagged) - Human LEM domain containing 3 (LEMD3), transcript variant 2 |
CNY 10,550.00 |
