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SMG7 (NM_001174061) Human Untagged Clone
CAT＃: SC329297
SMG7 (untagged)-Human Smg-7 homolog nonsense mediated mRNA decay factor (C. elegans) (SMG7) transcript variant 5
CNY 22,040.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C1orf16; EST1C; SGA56M |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC329297 representing NM_001174061.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTAGTTACCGATTTGGAATACGCTTTAGACAAGAAAGTAGAACAGGATCTCTGGAATCACGCCTTT AAGAATCAGATCACAACACTGCAAGGCCAGGCAAAGAATCGAGCAAATCCGAATCGGAGTGAAGTTCAG GCAAACCTTTCTCTGTTCCTAGAGGCAGCTAGTGGCTTCTATACTCAGTTATTACAAGAACTGTGTACA GTATTTAATGTAGATTTACCATGCCGTGTGAAGTCTTCCCAATTGGGAATTATCAGCAATAAACAGACG CATACCAGCGCCATAGTGAAGCCACAGTCTAGCTCCTGTTCCTATATCTGCCAGCACTGCCTCGTCCAC CTTGGAGACATTGCTCGATACAGAAACCAGACCAGCCAGGCAGAGTCCTACTATAGGCATGCAGCTCAG CTTGTCCCCTCCAATGGTCAGCCTTATAATCAGTTGGCTATCTTAGCTTCTTCCAAAGGAGACCATCTG ACCACAATTTTCTACTACTGCAGAAGCATTGCTGTGAAGTTCCCTTTCCCAGCTGCCTCCACTAATCTG CAAAAAGCACTTTCTAAAGCACTGGAAAGCCGAGATGAGGTGAAAACCAAGTGGGGTGTTTCTGACTTC ATCAAGGCCTTTATTAAATTCCACGGTCATGTGTACCTGAGTAAGAGCTTGGAAAAGTTGAGCCCTCTT CGAGAGAAATTGGAAGAACAGTTTAAGAGGCTGCTATTCCAAAAAGCTTTCAACTCTCAGCAGTTAGTT CATGTCACTGTCATTAACCTGTTTCAACTTCATCACCTTCGTGACTTTAGCAATGAAACCGAGCAGCAC ACTTATAGCCAAGATGAGCAGCTATGTTGGACACAGTTGCTGGCCCTCTTTATGTCTTTTCTTGGCATC CTGTGCAAGTGTCCTCTACAGAATGAGTCTCAGGAGGAGTCCTACAATGCCTATCCTCTTCCAGCAGTC AAGGTCTCCATGGACTGGCTAAGACTCAGACCCAGGGTCTTTCAGGAGGCAGTGGTGGATGAAAGACAG TACATTTGGCCCTGGTTGATTTCTCTTCTGAATAGTTTCCATCCCCATGAAGAGGACCTCTCAAGTATT AGTGCGACACCACTTCCAGAGGAGTTTGAATTACAAGGATTTTTGGCATTGAGACCTTCTTTCAGGAAC TTGGATTTTTCCAAAGGTCACCAGGGTATTACAGGGGACAAAGAAGGCCAGCAACGACGAATACGACAG CAACGCTTGATCTCTATAGGCAAATGGATTGCTGATAATCAGCCAAGGCTGATTCAGTGTGAAAATGAG GTAGGGAAATTGTTGTTTATCACAGAAATCCCAGAATTAATACTGGAAGACCCCAGTGAAGCCAAAGAG AACCTCATTCTGCAAGAAACATCTGTGATAGAGTCGCTGGCTGCAGATGGGAGCCCAGGGCTAAAATCA GTGCTATCTACAAGCCGAAATTTAAGCAACAACTGTGACACAGGAGAGAAGCCAGTGGTTACCTTCAAA GAAAACATTAAGACACGAGAAGTGAACAGAGACCAAGGAAGAAGTTTTCCTCCCAAAGAGGTGAGAAGG GACTATAGCAAAGGAATAACTGTAACTAAGAATGATGGAAAGAAGGACAACAACAAGAGGAAAACTGAA ACCAAGAAATGCACCTTAGAAAAGTTACAGGAAACAGGAAAGCAGAATGTGGCAGTGCAGGTAAAATCC CAGACAGAACTAAGAAAGACTCCAGTGTCTGAAGCCAGAAAAACACCTGTAACTCAAACCCCAACTCAA GCAAGTAACTCCCAGTTCATCCCCATTCATCACCCTGGAGCCTTCCCTCCTCTTCCCAGCAGGCCAGGG TTTCCGCCCCCAACATATGTTATCCCCCCGCCTGTGGCATTTTCTATGGGCTCAGGTTACACCTTCCCA GCTGGTGTTTCTGTCCCAGGAACCTTTCTTCAGCCTACAGCTCACTCTCCAGCAGGAAACCAGGTGCAA GCTGGGAAACAGTCCCACATTCCTTACAGCCAGCAACGGCCCTCTGGACCAGGGCCAATGAACCAGGGA CCTCAACAATCACAGCCACCTTCCCAGCAACCCCTTACATCTTTACCAGCTCAGCCAACAGCACAGTCT ACAAGCCAGCTGCAGGTTCAAGCTCTAACTCAGCAACAACAATCCCCTACAAAAGCTGTGCCGGCTTTG GGGAAAAGCCCGCCTCACCACTCTGGATTCCAGCAGTATCAACAGGCAGATGCCTCCAAACAGCTGTGG AATCCCCCTCAGGTTCAAGGCCCATTAGGGAAAATTATGCCTGTGAAACAGCCCTACTACCTTCAGACC CAAGACCCCATAAAACTGTTTGAGCCGTCATTGCAACCTCCTGTAATGCAGCAGCAGCCTCTAGAAAAA AAAATGAAGCCTTTTCCCATGGAGCCATATAACCATAATCCCTCAGAAGTCAAGGTCCCAGAATTCTAC TGGGATTCTTCCTACAGCATGGCTGATAACAGATCTGTAATGGCACAGCAAGCAAACATAGACCGCAGG GGCAAACGGTCACCAGGAGTCTTCCGTCCAGAGCAGGATCCTGTACCCAGAATGCCGTTTGAGGACCCC AAGAGCTCCCCTCTGCTTCCTCCGGACCTGTTAAAGAGTCTGGCTGCCTTGGAGGAAGAGGAAGAGCTG ATTTTTTCTAACCCTCCTGATCTTTACCCGGCTCTGCTGGGGCCTCTCGCCTCTCTTCCTGGACGAAGC CTTTTTAAATCCTTATTGGAGAAGCCCTCAGAGCTCATGTCACATTCATCCTCTTTCCTGTCCCTCACC GGATTCTCTCTCAATCAGGAAAGATACCCAAATAATAGTATGTTCAATGAGGTATATGGGAAAAACCTG ACATCCAGCTCCAAAGCAGAACTCAGTCCCTCAATGGCCCCCCAGGAAACATCTCTGTATTCCCTTTTT GAAGGGACTCCGTGGTCTCCATCACTTCCTGCCAGTTCAGATCATTCAACACCAGCCAGCCAGTCTCCT CATTCCTCTAACCCAAGCAGCCTACCCAGCTCTCCTCCAACACACAACCATAATTCTGTTCCATTCTCC AATTTTGGACCCATTGGGACTCCAGATAACAGGGATAGAAGGACTGCAGATCGGTGGAAAACTGATAAG CCAGCCATGGGTGGGTTTGGCATTGATTATCTCTCAGCAACGTCATCCTCTGAGAGCAGTTGGCATCAG GCCAGCACTCCGAGTGGCACCTGGACAGGCCATGGCCCTTCCATGGAGGATTCCTCTGCTGTCCTCATG GAAAGCCTAAAGTCTATCTGGTCCAGTTCCATGATGCATCCTGGACCTTCTGCTCTGGAGCAGCTGTTA ATGCAGCAGAAGCAGAAACAGCAACGGGGACAAGGCACCATGAACCCTCCACACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001174061 |
| Insert Size | 3438 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001174061.1 |
| RefSeq Size | 6054 bp |
| RefSeq ORF | 3438 bp |
| Locus ID | 9887 |
| UniProt ID | Q92540 |
| MW | 127.9 kDa |
| Gene Summary | This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. This variant also contains an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230659 | SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5 |
CNY 7,976.00 |
|
| RC230659L3 | Lenti-ORF clone of SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5 |
CNY 10,260.00 |
|
| RC230659L4 | Lenti-ORF clone of SMG7 (mGFP-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5 |
CNY 10,260.00 |
|
| RG230659 | SMG7 (tGFP-tagged) - Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5 |
CNY 9,220.00 |
