CENPO (NM_001199803) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CENP-O; ICEN-36; MCM21R
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329567 representing NM_001199803. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGGAATTCTCGCTTCTGGCCTGGGTGTTTTAGCTCACTTGGAAAGGCTAGAGACCCAAGTGAGC AGATCCCGTAAACAGTCTGAAGAGCTGCAGAGCGTGCAGGCCCAGGAAGGTGCTCTTGGAACCAAGATT CATAAACTAAGGCGTCTGCGAGATGAGCTGAGGGCTGTGGTGCGGCACCGGCGAGCCAGCGTGAAAGCA TGTATTGCCAATGTAGAACCCAACCAAACAGTGGAGATCAATGAGCAAGAAGCATTGGAAGAGAAATTG GAAAATGTGAAAGCCATTCTGCAGGCATATCATTTTACAGGCCTCAGTGGTAAACTGACCAGCCGAGGA GTTTGTGTCTGCATCAGTACTGCTTTTGAGGGGAACCTATTGGATTCCTATTTTGTGGACCTTGTCATA CAGAAACCACTCCGGATACATCACCATTCAGTCCCAGTCTTCATTCCCCTGGAAGAGATAGCTGCAAAA TATTTACAGACCAACATCCAGCACTTCCTGTTCAGTCTCTGCGAGTACCTGAATGCTTACTCTGGGAGG AAGTACCAGGCAGACCGGCTTCAGAGTGACTTTGCAGCCCTCCTGACTGGGCCCTTGCAGAGAAACCCA CTGTGTAACTTGCTGTCATTTACTTACAAACTGGATCCAGGGGGTCAGTCCTTCCCGTTCTGTGCTAGA TTGCTGTATAAGGACCTCACAGCAACTCTTCCCACTGACGTCACCGTGACATGTCAAGGAGTGGAAGTA TTATCCACTTCATGGGAGGAGCAACGAGCATCTCATGAAACTCTGTTCTGTACGAAGCCCTTGCATCAA GTGTTTGCCTCATTTACAAGAAAAGGAGAAAAGTTGGATATGAGTCTGGTCTCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199803
Insert Size	885 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199803.1
RefSeq Size	4125 bp
RefSeq ORF	885 bp
Locus ID	79172
UniProt ID	Q9BU64
MW	33 kDa
Gene Summary	This gene encodes a component of the interphase centromere complex. The encoded protein is localized to the centromere throughout the cell cycle and is required for bipolar spindle assembly, chromosome segregation and checkpoint signaling during mitosis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (2) differs in the 5' UTR, lacks an exon in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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