DHPS (NM_001206974) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DHS; DS; MIG13; NEDSSWI
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329867 representing NM_001206974. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCTATAATCCCAGCATTTTGGGAAGCCGAGGCGGGTGGATCAAGAGAAGAGGAGTTCGAGACAAGC CTGGCCAACATGATCGAGAAGAAGCTGGAACCACTGTCACAGGATGAAGACCAGCACGCGGACCTGACC CAGAGCCGCCGCCCACTTACCAGCTGCACCATTTTCCTGGGATATACATCCAACCTCATCAGTTCAGGC ATCCGTGAGACCATTCGCTACCTTGTGCAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGC GTGGAGGAAGACCTCATCAAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGGGGGAAGGAG CTCCGGGAGAACGGGATCAATAGGATCGGAAACCTGCTGGTGCCCAATGAGAATTACTGCAAGTTTGAG GACTGGCTGATGCCCATTCTGGACCAGATGGTGATGGAGCAGAACACAGAGGGTGTAAAGTGGACGCCT TCTAAGATGATCGCCCGGCTGGGCAAGGAGATCAACAACCCAGAGTCCGTGTATTACTGGGCCCAGAAG AACCACATCCCTGTGTTTAGTCCCGCACTTACAGACGGCTCGCTGGGCGACATGATCTTCTTCCATTCC TACAAGAACCCGGGCCTGGTCCTGGACATCGTTGAGGACCTGAGGCTCATCAACACACAGGCCATCTTT GCCAAGTGCACTGGGATGATCATTCTGGGCGGGGGCGTGGTCAAGCACCACATTGCCAATGCCAACCTC ATGCGGAACGGGGCCGACTACGCTGTTTACATCAACACAGCCCAGGAGTTTGATGGCTCTGACTCAGGT GCCCGACCAGACGAGGCTGTCTCCTGGGGCAAGATCCGGGTGGATGCACAGCCCGTCAAGGTCTATGCT GACGCCTCCCTGGTCTTCCCCCTGCTTGTGGCTGAAACCTTTGCCCAGAAGATGGATGCCTTCATGCAT GAGAAGAACGAGGACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001206974
Insert Size	984 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001206974.1
RefSeq Size	1212 bp
RefSeq ORF	984 bp
Locus ID	1725
UniProt ID	P49366
MW	36.6 kDa
Gene Summary	This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (d) is shorter and has a distinct N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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