ATP dependent metalloprotease YME1L1 (YME1L1) (NM_001253866) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FTSH; MEG4; OPA11; PAMP; YME1L
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330313 representing NM_001253866. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTTTCCTTGTCGAGCACGGTGCAACCCCAGGTTACAGTTCCTCTGAGTCATCTCATCAATGCCTTC CATACACCAAAAAACACTTCTGTTTCTCTCAGTGGAGTGTCAGTTTCTCAAAACCAGCATCGAGATGTA GTTCCTGAGCATGAGGCTCCCAGCAGTGAGCCTTCACTTAACTTAAGGGACCTTGGATTATCTGAACTA AAAATTGGACAGATTGATCAGCTGGTAGAAAATCTACTTCCTGGATTTTGTAAAGGCAAAAACATTTCT TCCCATTGGCATACATCCCATGTCTCTGCACAATCCTTCTTTGAAAATAAATATGTTTTCATACAGTCT CGGGGTTTTAAAACTTTGAAATCAAGGACACGACGTCTCCAGTCTACCTCCGAGAGATTAGCTGAAACA CAGAATATAGCGCCATCATTCGTGAAGGGGTTTCTTTTGCGGGACAGAGGATCAGATGTTGAGAGTTTG GACAAACTCATGAAAACCAAAAATATACCTGAAGCTCACCAAGATGCATTTAAAACTGGTTTTGCGGAA GGTTTTCTGAAAGCTCAAGCACTCACACAAAAAACCAATGATTCCCTAAGGCGAACCCGTCTGATTCTC TTCGTTCTGCTGCTATTCGGCATTTATGGACTTCTAAAAAACCCATTTTTATCTGTCCGCTTCCGGACA ACAACAGGGCTTGATTCTGCAGTAGATCCTGTCCAGATGAAAAATGTCACCTTTGAACATGTTAAAGGG GTGGAGGAAGCTAAACAAGAATTACAGGAAGTTGTTGAATTCTTGAAAAATCCACAAAAATTTACTATT CTTGGAGGTAAACTTCCAAAAGGAATTCTTTTAGTTGGACCCCCAGGGACTGGAAAGACACTTCTTGCC CGAGCTGTGGCGGGAGAAGCTGATGTTCCTTTTTATTATGCTTCTGGATCCGAATTTGATGAGATGTTT GTGGGTGTGGGAGCCAGCCGTATCAGAAATCTTTTTAGGGAAGCAAAGGCGAATGCTCCTTGTGTTATA TTTATTGATGAATTAGATTCTGTTGGTGGGAAGAGAATTGAATCTCCAATGCATCCATATTCAAGGCAG ACCATAAATCAACTTCTTGCTGAAATGGATGGTTTTAAACCCAATGAAGGAGTTATCATAATAGGAGCC ACAAACTTCCCAGAGGCATTAGATAATGCCTTAATACGTCCTGGTCGTTTTGACATGCAAGTTACAGTT CCAAGGCCAGATGTAAAAGGTCGAACAGAAATTTTGAAATGGTATCTCAATAAAATAAAGTTTGATCAA TCCGTTGATCCAGAAATTATAGCTCGAGGTACTGTTGGCTTTTCCGGAGCAGAGTTGGAGAATCTTGTG AACCAGGCTGCATTAAAAGCAGCTGTTGATGGAAAAGAAATGGTTACCATGAAGGAGCTGGAGTTTTCC AAAGACAAAATTCTAATGGGGCCTGAAAGAAGAAGTGTGGAAATTGATAACAAAAACAAAACCATCACA GCATATCATGAATCTGGTCATGCCATTATTGCATATTACACAAAAGATGCAATGCCTATCAACAAAGCT ACAATCATGCCACGGGGGCCAACACTTGGACATGTGTCCCTGTTACCTGAGAATGACAGATGGAATGAA ACTAGAGCCCAGCTGCTTGCACAAATGGATGTTAGTATGGGAGGAAGAGTGGCAGAGGAGCTTATATTT GGAACCGACCATATTACAACAGGTGCTTCCAGTGATTTTGATAATGCCACTAAAATAGCAAAGCGGATG GTTACCAAATTTGGAATGAGTGAAAAGCTTGGAGTTATGACCTACAGTGATACAGGGAAACTAAGTCCA GAAACCCAATCTGCCATCGAACAAGAAATAAGAATCCTTCTAAGGGACTCATATGAACGAGCAAAACAT ATCTTGAAAACTCATGCAAAGGAGCATAAGAATCTCGCAGAAGCTTTATTGACCTATGAGACTTTGGAT GCCAAAGAGATTCAAATTGTTCTTGAGGGGAAAAAGTTGGAAGTGAGATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001253866
Insert Size	2052 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253866.1
RefSeq Size	4133 bp
RefSeq ORF	2052 bp
Locus ID	10730
UniProt ID	Q96TA2
Protein Families	Druggable Genome, Protease
MW	76 kDa
Gene Summary	The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (4) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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