MAG1 (GPAT3) (NM_001256421) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AGPAT8; AGPAT9; AGPAT 10; AGPAT10; HMFN0839; LPAAT-theta; MAG1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330424 representing NM_001256421. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGGGCGCAGAGCTGGCCGGGAAGATCCTTTCCACCTGGCTGACGCTGGTTCTCGGCTTCATCCTT TTACCTTCGGTCTTCGGAGTGTCTCTGGGCATCTCCGAGATCTACATGAAGATCCTAGTGAAAACTTTA GAGTGGGCCACAATACGAATTGAAAAAGGAACCCCAAAGGAGTCGATTCTTAAAAACTCTGCTTCTGTT GGTATTATCCAAAGAGATGAGTCACCCATGGAAAAAGGGCTCTCTGGTCTACGAGGAAGGGACTTTGAG CTGTCTGACGTGTTTTATTTCTCCAAGAAGGGATTGGAAGCCATTGTAGAAGATGAAGTGACCCAGAGG TTTTCCTCAGAGGAGCTAGTGTCATGGAATCTCCTCACAAGAACCAATGTAAATTTCCAGTACATCAGT CTGCGGCTCACTATGGTGTGGGTGCTGGGCGTCATAGTGCGCTATTGTGTCCTACTGCCTCTGAGGGTT ACCTTGGCTTTCATTGGGATCAGTTTGCTGGTTATAGGAACTACACTGGTTGGGCAGCTGCCAGACAGC AGCCTCAAAAACTGGCTGAGTGAACTGGTCCATCTGACTTGCTGCCGGATCTGTGTGCGAGCCCTCTCT GGTACCATTCATTATCATAACAAGCAGTACAGACCCCAGAAGGGAGGCATTTGTGTTGCCAACCATACT TCCCCCATTGATGTTTTAATCTTGACAACGGATGGATGTTATGCTATGGTTGGCCAGGTTCATGGCGGC TTGATGGGAATTATTCAGAGAGCTATGGTCAAGGCTTGTCCTCATGTCTGGTTTGAACGCTCAGAAATG AAGGATCGACACCTGGTTACTAAGAGACTAAAAGAACATATTGCTGATAAGAAGAAACTACCCATACTA ATTTTTCCTGAAGGAACTTGCATCAACAATACTTCAGTCATGATGTTTAAAAAGGGGAGCTTTGAAATT GGAGGAACCATACATCCAGTTGCAATTAAGTATAACCCTCAGTTCGGTGATGCATTTTGGAACAGTAGT AAATACAACATGGTGAGCTACCTGCTTCGAATGATGACCAGCTGGGCCATCGTCTGTGACGTGTGGTAC ATGCCCCCCATGACCAGAGAGGAAGGAGAAGATGCAGTCCAGTTTGCTAACAGGGTTAAGTCTGCTATT GCTATACAAGGAGGCCTGACTGAACTTCCCTGGGATGGAGGACTAAAGAGAGCAAAGGTGAAGGACATC TTTAAGGAAGAGCAGCAGAAAAATTACAGCAAGATGATTGTGGGCAATGGATCTCTCAGCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256421
Insert Size	1305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256421.1
RefSeq Size	2640 bp
RefSeq ORF	1305 bp
Locus ID	84803
UniProt ID	Q53EU6
Protein Families	Transmembrane
MW	48.7 kDa
Gene Summary	This gene encodes a member of the lysophosphatidic acid acyltransferase protein family. The encoded protein is an enzyme which catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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