CD300 antigen (CD300A) (NM_001256841) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CLM-8; CMRF-35-H9; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF35H; CMRF35H9; IGSF12; IRC1; IRC1/IRC2; IRC2; IRp60
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330532 representing NM_001256841. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTGGCTGCCTTGGGCTCTGTTGCTTCTCTGGGTCCCAGCATCAACGTCAATGACACCTGCAAGTATC ACTGCGGCCAAGACCTCAACAATCACAACTGCATTTCCACCTGTATCATCCACTACCCTGTTTGCAGTG GGTGCCACCCACAGTGCCAGCATCCAGGAGGAAACTGAGGAGGTGGTGAACTCACAGCTCCCGCTGCTC CTCTCCCTGCTGGCATTGTTGCTGCTTCTGTTGGTGGGGGCCTCCCTGCTAGCCTGGAGGATGTTTCAG AAATGGATCAAAGCTGGTGACCATTCAGAGCTGTCCCAGAACCCCAAGCAGGCTGCCACGCAGAGTGAG CTGCACTACGCAAATCTGGAGCTGCTGATGTGGCCTCTGCAGGAAAAGCCAGCACCACCAAGGGAGGTG GAGGTGGAATACAGCACTGTGGCCTCCCCCAGGGAAGAACTTCACTATGCCTCGGTGGTGTTTGATTCT AACACCAACAGGATAGCTGCTCAGAGGCCTCGGGAGGAGGAACCAGATTCAGATTACAGTGTGATAAGG AAGACATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001256841
Insert Size	561 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256841.1
RefSeq Size	1554 bp
RefSeq ORF	561 bp
Locus ID	11314
UniProt ID	Q9UGN4
Protein Families	Transmembrane
MW	20.6 kDa
Gene Summary	This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (2) lacks an exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2), also referred to as IRC1c, is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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