WTAP (NM_001270532) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Mum2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330858 representing NM_001270532. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGACCAACGAAGAACCTCTTCCCAAGAAGGTTCGATTGAGTGAAACAGACTTCAAAGTTATGGCAAGA GATGAGTTAATTCTAAGATGGAAACAATATGAAGCATATGTACAAGCTTTGGAGGGCAAGTACACAGAT CTTAACTCTAATGATGTAACTGGCCTAAGAGAGTCTGAAGAAAAACTAAAGCAACAACAGCAGGAGTCT GCACGCAGGGAAAACATCCTTGTAATGCGACTAGCAACCAAGGAACAAGAGATGCAAGAGTGTACTACT CAAATCCAGTACCTCAAGCAAGTCCAGCAGCCGAGCGTTGCCCAACTGAGATCAACAATGGTAGACCCA GCGATCAACTTGTTTTTCCTAAAAATGAAAGGTGAACTGGAACAGACTAAAGACAAACTGGAACAAGCC CAAAATGAACTGAGTGCCTGGAAGTTTACGCCTGATAGAGGCCTGATGGCGTCGGACTATTCCGAAGAA GTGGCCACCTCCGAAAAATTCCCCTTCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001270532
Insert Size	513 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270532.1
RefSeq Size	1636 bp
RefSeq ORF	513 bp
Locus ID	9589
UniProt ID	Q15007
Protein Families	Druggable Genome
MW	19.9 kDa
Gene Summary	The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (5) has an alternate 3' end sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Variants 5 and 6 both encode the same isoform (3).

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