APEX2 (NM_001271748) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APE2; APEXL2; XTH2; ZGRF2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330961 representing NM_001271748. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCGCTTCTATCGTTTGCTGCAAATCCGAGCAGAAGCCCTCCTGGCGGCAGGCAGCCATGTGATCATT CTGGGTGACCTGAATACAGCCCACCGCCCCATTGACCACTGGGATGCAGTCAACCTGGAATGCTTTGAA GAGGACCCAGGGCGCAAGTGGATGGACAGCTTGCTCAGTAACTTGGGGTGCCAGTCTGCCTCTCATGTA GGGCCCTTCATCGATAGCTACCGCTGCTTCCAACCAAAGCAGGAGGGGGCCTTCACCTGCTGGTCAGCA GTCACTGGCGCCCGCCATCTCAACTATGGCTCCCGGCTTGACTATGTGCTGGGGGACAGGACCCTGGTC ATAGACACCTTTCAGGCCTCTTTCCTGCTGCCTGAGGTGATGGGCTCTGACCACTGCCCTGTGGGTGCA GTCTTGAGTGTGTCCTCTGTGCCTGCAAAACAGTGCCCACCTCTGTGCACCCGCTTCCTCCCTGAGTTT GCAGGCACCCAGCTCAAGATCCTTCGCTTCCTAGTTCCTCTCGAACAAAGTCCTGTGTTGGAGCAGTCG ACGCTGCAGCACAACAATCAAACCCGGGTACAGACATGCCAAAACAAAGCCCAAGTGCGCTCAACCAGG CCTCAGCCCAGTCAGGTTGGCTCTAGCAGAGGCCAGAAAAACCTGAAGAGCTACTTTCAGCCCTCCCCT AGCTGTCCCCAAGCCTCTCCTGACATAGAGCTGCCTAGCCTACCACTGATGAGCGCCCTCATGACCCCG AAGACTCCAGAAGAGAAGGCAGTGGCCAAAGTGGTGAAGGGGCAGGCCAAGACTTCAGAAGCCAAAGAT GAGAAGGAGTTACGGACCTCATTCTGGAAGTCTGTGCTGGCGGGGCCCTTGCGCACACCCCTCTGTGGG GGCCACAGGGAGCCATGTGTGATGCGTACTGTGAAGAAGCCAGGACCCAACTTGGGCCGCCGCTTCTAC ATGTGTGCCAGGCCCCGGGGTCCTCCCACTGACCCCTCCTCCCGGTGCAACTTCTTCCTCTGGAGCAGG CCCAGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271748
Insert Size	1044 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271748.1
RefSeq Size	1914 bp
RefSeq ORF	1044 bp
Locus ID	27301
Protein Families	Druggable Genome
Protein Pathways	Base excision repair
MW	38.5 kDa
Gene Summary	Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (2) lacks a 5' exon compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG. The encoded protein (isoform 2) has a shorter N-terminus, compared to isoform 1.

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