DCDC2 (NM_001195610) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DCDC2A; DFNB66; NPHP19; NSC; RU2; RU2S
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331190 representing NM_001195610. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGCGGCAGCAGCGCCAGGTCCAGCCACCTGTCTCAGCCCGTCGTGAAGAGCGTGCTTGTGTACCGC AACGGGGACCCCTTCTACGCGGGGCGCCGCGTCGTCATCCATGAGAAGAAGGTGTCCAGCTTCGAAGTC TTCCTGAAGGAGGTGACCGGCGGCGTTCAGGCACCCTTTGGGGCCGTCAGGAACATCTACACCCCGCGG ACTGGCCACCGAATCCGGAAGCTAGACCAGATCCAGAGCGGGGGCAATTACGTGGCTGGAGGCCAGGAA GCCTTCAAGAAACTCAATTACTTGGACATAGGAGAAATCAAGAAAAGACCAATGGAAGTTGTTAATACA GAGGTAAAACCAGTAATCCATAGCAGGATCAACGTGTCAGCTCGCTTTAGAAAACCGCTTCAGGAGCCG TGCACTATCTTCTTGATTGCAAATGGAGACCTCATAAACCCAGCTTCTCGCCTCCTTATCCCCAGAAAA ACCTTGAATCAGTGGGATCATGTACTACAAATGGTCACAGAAAAAATCACTCTGAGGAGCGGGGCTGTT CACAGGCTTTATACTTTAGAAGGAAAACTTGTTGAGAGTGGAGCAGAGTTGGAGAATGGGCAGTTTTAT GTGGCTGTTGGCAGAGATAAGTTTAAGAAACTGCCTTACAGTGAGTTACTTTTTGACAAGTCAACGATG AGAAGGCCTTTTGGTCAGAAAGCTTCTTCACTACCTCCTATTGTAGGATCCAGAAAGTCTAAAGGGAGT GGAAATGATCGCCACTCTAAGTCAACAGTTGGATCCAGTGACAACTCATCTCCTCAGCCCCTGAAGAGG AAAGGGAAAAAAGAAGACGTGAATTCAGAAAAACTGACGAAATTGAAACAAAATGTAAAATTAAAGAAT TCACAAGAAACCATTCCAAATAGTGATGAAGGCATTTTCAAAGCTGGAGCAGAGAGGTCTGAAACACGG GGGGCAGCAGAAGTCCAAGAAGATGAAGATACTCAGGTTGAGGTTCCAGTCGATCAGAGGCCAGCAGAA ATAGTAGACGAGGAAGAAGATGGAGAGAAGGCAAACAAGGATGCAGAACAGAAAGAAGACTTTTCAGGA ATGAATGGTGACCTTGAAGAGGAAGGAGGTAGGGAGGCTACAGATGCCCCTGAGCAAGTCGAGGAGATT CTGGATCACAGTGAGCAGCAGGCACGCCCTGCTCGTGTAAATGGAGGCACCGATGAGGAGAATGGTGAG GAGCTGCAGCAGGTTAATAATGAGCTTCAACTGGTCCTAGACAAGGAAAGAAAGTCTCAAGGAGCTGGC AGTGGACAAGATGAGGCTGATGTAGACCCTCAAAGACCACCAAGGCCAGAAGTAAAAATTACCAGTCCA GAAGAAAATGAAAACAACCAACAAAACAAGGACTATGCTGCCGTGGCTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001195610
Insert Size	1431 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001195610.1
RefSeq Size	4623 bp
RefSeq ORF	1431 bp
Locus ID	51473
UniProt ID	Q9UHG0
MW	52.8 kDa
Gene Summary	This gene encodes a doublecortin domain-containing family member. The doublecortin domain has been demonstrated to bind tubulin and enhance microtubule polymerization. This family member is thought to function in neuronal migration where it may affect the signaling of primary cilia. Mutations in this gene have been associated with reading disability (RD) type 2, also referred to as developmental dyslexia. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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