DAAM2 (NM_001201427) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dJ90A20A.1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331422 representing NM_001201427. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCCCCCGCAAGAGGAGCCACCATGGCCTGGGCTTCCTGTGCTGCTTCGGGGGCAGTGACATCCCC GAAATCAACCTCCGGGACAACCACCCTCTGCAGTTCATGGAGTTCTCCAGCCCCATCCCGAACGCAGAG GAGCTCAACATCCGCTTTGCAGAGCTGGTGGATGAATTGGATCTCACTGACAAAAACCGAGAGGCTATG TTTGCACTGCCCCCTGAGAAGAAATGGCAGATCTACTGCAGCAAGAAGAAGGAGCAGGAGGACCCCAAC AAGCTGGCAACCAGCTGGCCTGACTATTACATCGACCGCATCAATTCCATGGCTGCGATGCAGAGTCTG TACGCGTTTGATGAGGAGGAGACGGAGATGAGGAACCAAGTCGTGGAAGACCTGAAGACAGCCCTCCGG ACACAGCCTATGAGGTTTGTGACCCGCTTCATTGAGCTGGAGGGCTTGACCTGTCTGCTAAATTTCCTC CGGAGCATGGACCACGCCACCTGTGAGAGCCGCATCCACACCTCACTCATTGGCTGCATCAAAGCATTG ATGAACAACTCCCAGGGGCGGGCACATGTGCTGGCACAGCCTGAGGCCATTAGTACCATAGCCCAGAGC CTACGCACAGAGAACAGCAAGACCAAGGTGGCTGTGCTGGAGATCCTGGGTGCTGTGTGCCTCGTGCCT GGTGGCCACAAGAAGGTGCTGCAGGCCATGCTGCACTACCAGGTGTATGCAGCAGAGCGAACCCGCTTC CAGACCCTGCTGAACGAGCTAGACCGAAGTCTGGGCCGGTACCGGGATGAAGTGAATCTGAAAACAGCC ATCATGTCCTTCATCAATGCTGTCCTCAATGCTGGAGCTGGAGAGGATAATCTGGAGTTCCGCCTACAT CTACGGTATGAATTCCTGATGCTGGGTATACAGCCTGTGATTGACAAGCTCCGGCAACATGAAAATGCC ATCCTGGACAAACATTTAGACTTCTTCGAGATGGTGCGGAATGAGGATGACCTGGAGCTAGCCAGGAGG TTTGACATGGTCCACATCGACACCAAGAGTGCTTCCCAGATGTTTGAGTTGATCCACAAGAAGCTGAAG TACACGGAGGCCTACCCCTGCCTGCTCTCTGTGCTGCACCACTGCCTGCAGATGCCCTACAAACGGAAC GGTGGCTACTTCCAGCAGTGGCAGCTCCTGGACCGCATCCTCCAGCAGATTGTCCTCCAGGATGAGCGG GGTGTGGACCCTGACCTGGCTCCCTTGGAGAACTTCAATGTCAAGAACATCGTCAACATGCTCATCAAC GAGAATGAAGTGAAACAGTGGCGAGACCAGGCAGAGAAGTTCCGGAAAGAACACATGGAGCTTGTGAGC CGTCTGGAGAGGAAGGAGCGGGAATGCGAGACAAAGACATTGGAGAAGGAAGAGATGATGCGGACGCTG AACAAAATGAAGGACAAGCTGGCCCGGGAGTCCCAGGAGCTGCGCCAGGCTCGGGGACAAGTGGCAGAG CTGGTAGCCCAGCTCAGTGAACTCTCAACAGGCCCTGTATCTTCCCCACCACCCCCTGGGGGCCCACTC ACCTTGTCTTCCTCAATGACAACCAATGACCTGCCTCCACCCCCTCCTCCTCTGCCCTTTGCCTGTTGT CCCCCTCCCCCACCACCACCCCTTCCTCCCGGGGGACCCCCGACTCCCCCAGGTGCCCCACCTTGCCTC GGCATGGGCCTGCCCCTCCCTCAGGACCCCTACCCCAGCAGTGACGTCCCACTCAGGAAAAAGCGTGTC CCCCAGCCTTCTCACCCACTGAAGTCCTTCAACTGGGTGAAGCTGAATGAGGAGCGTGTCCCTGGCACC GTATGGAATGAGATTGATGACATGCAGGTATTTCGGATCCTGGACCTAGAGGATTTTGAAAAGATGTTT TCAGCCTACCAGAGGCACCAGAAAGAGCTGGGCTCCACTGAAGACATCTACCTGGCTTCCCGCAAGGTC AAAGAGCTGTCGGTCATTGATGGCCGGAGGGCCCAAAACTGCATCATCCTTCTTTCCAAGTTGAAGCTT TCTAACGAGGAGATCCGGCAGGCCATCTTGAAGATGGATGAGCAGGAGGACCTTGCTAAGGACATGCTG GAGCAGCTCCTCAAGTTCATCCCAGAGAAGAGTGACATTGACCTCCTGGAGGAGCACAAGCATGAAATT GAGCGGATGGCCCGTGCTGACCGCTTCCTCTATGAAATGAGCAGGATTGACCACTACCAGCAGCGACTG CAAGCCCTCTTCTTCAAGAAGAAATTCCAGGAGCGGCTGGCTGAGGCAAAGCCCAAAGTGGAAGCCATC CTGTTGGCCTCCCGGGAGCTGGTCCGCAGCAAGCGTCTTAGACAGATGCTAGAGGTCATCCTAGCCATA GGCAACTTCATGAACAAAGGGCAGCGTGGGGGCGCCTACGGGTTCCGGGTGGCCAGCCTCAACAAGATC GCTGACACCAAGTCCAGCATCGACAGAAACATCTCTCTGCTCCATTACCTGATCATGATCCTGGAGAAG CATTTTCCTGATATTCTAAACATGCCTTCAGAGCTGCAACATCTTCCAGAAGCTGCCAAAGTCAACCTA GCAGAACTGGAGAAGGAGGTGGGCAACCTCAGGAGGGGCCTGAGAGCGGTGGAGGTGGAGCTGGAGTAT CAGAGGCGCCAGGTACGGGAGCCCAGTGACAAGTTTGTCCCTGTCATGAGCGACTTCATCACGGTGTCC AGCTTCAGCTTCTCCGAGCTGGAGGACCAGCTAAATGAGGCCAGGGACAAGTTCGCCAAGGCCTTGATG CACTTCGGGGAGCATGACAGCAAGATGCAGCCAGACGAATTCTTTGGCATCTTTGATACCTTCTTGCAG GCCTTCTCAGAGGCCCGGCAGGATCTAGAGGCCATGAGGAGGAGGAAGGAGGAGGAGGAGCGGCGGGCG CGCATGGAAGCCATGCTGAAGGAGCAGAGGGAACGTGAGCGGTGGCAGCGGCAGCGGAAGGTCCTGGCT GCAGGCAGCTCGCTGGAGGAGGGAGGAGAGTTCGATGACCTGGTGTCGGCCCTGCGCTCTGGGGAGGTC TTCGACAAGGACTTATGCAAGCTCAAGCGCAGCCGCAAGCGATCAGGGAGCCAGGCCCTGGAAGTTACC CGGGAGCGGGCAATAAACCGGCTAAATTATTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001201427
Insert Size	3207 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001201427.1
RefSeq Size	6191 bp
RefSeq ORF	3207 bp
Locus ID	23500
UniProt ID	Q86T65
Protein Pathways	Wnt signaling pathway
MW	123.5 kDa
Gene Summary	Key regulator of the Wnt signaling pathway, which is required for various processes during development, such as dorsal patterning, determination of left/right symmetry or myelination in the central nervous system. Acts downstream of Wnt ligands and upstream of beta-catenin (CTNNB1). Required for canonical Wnt signaling pathway during patterning in the dorsal spinal cord by promoting the aggregation of Disheveled (Dvl) complexes, thereby clustering and formation of Wnt receptor signalosomes and potentiating Wnt activity. During dorsal patterning of the spinal cord, inhibits oligodendrocytes differentiation via interaction with PIP5K1A. Also regulates non-canonical Wnt signaling pathway. Acts downstream of PITX2 in the developing gut and is required for left/right asymmetry within dorsal mesentery: affects mesenchymal condensation by lengthening cadherin-based junctions through WNT5A and non-canonical Wnt signaling, inducing polarized condensation in the left dorsal mesentery necessary to initiate gut rotation. Together with DAAM1, required for myocardial maturation and sarcomere assembly.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate in-frame splice site at the 5' end of a coding exon compared to isoform 1. The resulting variant (1) encodes the longer isoform.

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