Proprotein Convertase 2 (PCSK2) (NM_001201528) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NEC-2; NEC 2; NEC2; PC2; SPC2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331435 representing NM_001201528. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTTTTTGCATCTGCTGAGCGACCGGTCTTCACGAATCATTTTCTTGTGGAGTTGCATAAAGGGGGA GAGGACAAAGCTCGCCAAGTTGCAGCAGAACACGGCTTTGGAGTCCGAAAGCTTCCCTTTGCTGAAGGT CTGTACCACTTTTATCACAATGGCCTTGCAAAGGCCAAGAGAAGACGCAGCCTACACCACAAGCAGCAG CTGGAGAGAGACCCCAGGGTAAAGATGGCTTTGCAGCAGGAAGGATTTGACCGAAAAAAGCGAGGTTAC AGAGACATCAATGAGATCGACATCAACATGAACGATCCTCTTTTTACAAAGCAGTGGTATCTGATCAAT ACTGGGCAAGCTGATGGCACTCCTGGCCTTGATTTGAATGTGGCTGAAGCCTGGGAGCTGGGATACACA GGGAAAGGTGTTACCATTGGAATTATGGATGATGGGATTGACTATCTCCACCCGGACCTGGCCTCCAAC TATAATGCCGAAGCAAGTTACGACTTCAGCAGCAACGACCCCTATCCTTACCCTCGGTACACAGATGAC TGGTTTAACAGCCACGGGACCCGATGTGCAGGAGAAGTTTCTGCTGCCGCCAACAACAATATCTGTGGA GTTGGAGTAGCATACAACTCCAAGGTTGCAGGCATCCGGATGCTGGACCAGCCATTCATGACAGACATC ATCGAGGCCTCCTCCATCAGTCATATGCCACAGCTGATTGACATCTACAGCGCCAGCTGGGGCCCCACA GACAACGGCAAGACAGTGGATGGGCCCCGGGAGCTCACGCTGCAGGCCATGGCCGATGGCGTGAACAAG GGCCGCGGCGGCAAAGGCAGCATCTACGTGTGGGCCTCCGGGGACGGCGGCAGCTATGACGACTGCAAC TGCGACGGCTACGCCTCCAGCATGTGGACCATCTCCATCAACTCAGCCATCAACGACGGCAGGACTGCC CTGTACGACGAGAGCTGCTCTTCCACCTTGGCTTCCACCTTCAGCAACGGGAGGAAAAGGAACCCCGAG GCCGGTGTGGCAACCACAGATTTGTACGGCAACTGCACTCTGAGGCATTCTGGGACATCTGCAGCTGCC CCCGAGGCAGCTGGTGTGTTTGCACTGGCTCTGGAGGCTAACCTGGGTCTGACCTGGCGGGACATGCAG CATCTGACTGTGCTCACCTCCAAACGGAACCAGCTTCACGACGAGGTCCATCAGTGGCGGCGCAATGGG GTCGGCCTGGAATTTAATCACCTCTTTGGCTACGGGGTCCTTGATGCAGGTGCCATGGTGAAAATGGCT AAAGACTGGAAAACCGTGCCTGAGAGATTCCACTGTGTGGGAGGCTCCGTGCAGGACCCTGAGAAAATA CCATCCACTGGCAAGTTGGTGCTGACACTCACAACCGACGCCTGTGAGGGGAAGGAAAATTTTGTCCGC TACCTGGAGCATGTCCAGGCTGTCATCACGGTCAACGCAACCAGAAGAGGAGACCTGAACATCAACATG ACTTCCCCTATGGGCACCAAGTCCATTTTGCTGAGCCGGCGTCCAAGGGATGACGACTCCAAGGTGGGC TTTGACAAGTGGCCTTTCATGACCACTCACACGTGGGGGGAAGACGCCCGAGGCACCTGGACCCTGGAG CTGGGATTTGTCGGCAGCGCCCCGCAGAAGGGGGTGCTGAAGGAGTGGACCCTGATGCTGCATGGCACT CAGAGTGCCCCGTACATCGACCAGGTGGTGCGGGATTACCAGTCCAAGTTGGCCATGTCCAAGAAAGAG GAGCTGGAGGAAGAGCTGGACGAAGCCGTGGAGAGAAGCCTGAAAAGCATCCTTAACAAGAACTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001201528
Insert Size	1860 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001201528.1
RefSeq Size	4687 bp
RefSeq ORF	1860 bp
Locus ID	5126
UniProt ID	P16519
Protein Families	Druggable Genome, Protease, Secreted Protein
MW	68.6 kDa
Gene Summary	This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The protein undergoes an initial autocatalytic processing event and interacts with a neuroendocrine secretory protein in the ER, exits the ER and sorts to secretory granules, where it is cleaved and catalytically activated during intracellular transport. The encoded protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Single nucleotide polymorphisms in this gene may increase susceptibility to myocardial infarction and type 2 diabetes. This gene may also play a role in tumor development and progression. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (3) differs in the 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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