Bmi1 (COMMD3-BMI1) (NM_001204062) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BMI1; PCGF4; RNF51
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331486 representing NM_001204062. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGCTCTCGGAGTCTGTGCAGAAAGGCTTCCAGATGCTGGCGGATCCCCGCTCCTTCGACTCCAAC GCCTTCACGCTTCTCCTCCGGGCGGCATTCCAGAGTCTGCTGGACGCCCAGGCGGACGAGGCCGTGTTA GATCATCCAGACTTGAAACATATCGACCCAGTGGTTTTAAAACATTGTCATGCAGCAGCTGCAACTTAC ATACTAGAGGCAGGAAAGCACCGAGCTGACAAGTCAACTCTAAGCACTTATCTAGAAGACTGTAAATTT GACAGAGAGCGAATAGAACTGTTTTGCACGGAATATCAGAATAATAAGAATTCCCTAGAAATCCTACTG GGAAGAACACTGATTCCCCATCCTATCCAGAGATTAGTTTTAGTTGCAGCATGGAACAATTACAGGATT TTTTATCAAGCAGAAATGCATCGAACAACGAGAATCAAGATCACTGAGCTAAATCCCCACCTGATGTGT GTGCTTTGTGGAGGGTACTTCATTGATGCCACAACCATAATAGAATGTCTACATTCCTTCTGTAAAACG TGTATTGTTCGTTACCTGGAGACCAGCAAGTATTGTCCTATTTGTGATGTCCAAGTTCACAAGACCAGA CCACTACTGAATATAAGGTCAGATAAAACTCTCCAAGATATTGTATACAAATTAGTTCCAGGGCTTTTC AAAAATGAAATGAAGAGAAGAAGGGATTTTTATGCAGCTCATCCTTCTGCTGATGCTGCCAATGGCTCT AATGAAGATAGAGGAGAGGTTGCAGATGAAGATAAGAGAATTATAACTGATGATGAGATAATAAGCTTA TCCATTGAATTCTTTGACCAGAACAGATTGGATCGGAAAGTAAACAAAGACAAAGAGAAATCTAAGGAG GAGGTGAATGATAAAAGATACTTACGATGCCCAGCAGCAATGACTGTGATGCACTTAAGAAAGTTTCTC AGAAGTAAAATGGACATACCTAATACTTTCCAGATTGATGTCATGTATGAGGAGGAACCTTTAAAGGAT TATTATACACTAATGGATATTGCCTACATTTATACCTGGAGAAGGAATGGTCCACTTCCATTGAAATAC AGAGTTCGACCTACTTGTAAAAGAATGAAGATCAGTCACCAGAGAGATGGACTGACAAATGCTGGAGAA CTGGAAAGTGACTCTGGGAGTGACAAGGCCAACAGCCCAGCAGGAGGTATTCCCTCCACCTCTTCTTGT TTGCCTAGCCCCAGTACTCCAGTGCAGTCTCCTCATCCACAGTTTCCTCACATTTCCAGTACTATGAAT GGAACCAGCAACAGCCCCAGCGGTAACCACCAATCTTCTTTTGCCAATAGACCTCGAAAATCATCAGTA AATGGGTCATCAGCAACTTCTTCTGGTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001204062
Insert Size	1410 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204062.1
RefSeq Size	3388 bp
RefSeq ORF	1410 bp
Locus ID	100532731
UniProt ID	P35226
MW	53.3 kDa
Gene Summary	This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011]

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