SP100 (NM_001206702) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	lysp100b
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331673 representing NM_001206702. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCAGGTGGGGGCGGCGACCTGAGCACCAGGAGGCTGAATGAATGTATTTCACCAGTAGCAAATGAG ATGAACCATCTTCCTGCACACAGCCACGATTTGCAAAGGATGTTCACGGAAGACCAGGGTGTAGATGAC AGGCTGCTCTATGACATTGTATTCAAGCACTTCAAAAGAAATAAGGTGGAGATTTCAAATGCAATAAAA AAGACATTTCCATTCCTCGAGGGCCTCCGTGATCGTGATCTCATCACAAATAAAATGTTTGAAGATTCT CAAGATTCTTGTAGAAACCTGGTCCCTGTACAGAGAGTGGTGTACAATGTTCTTAGTGAACTGGAGAAG ACATTTAACCTGCCAGTTCTGGAAGCACTGTTCAGCGATGTCAACATGCAGGAATACCCCGATTTAATT CACATTTATAAAGGCTTTGAAAATGTAATCCATGACAAATTGCCTCTCCAAGAAAGTGAAGAAGAAGAG AGGGAGGAGAGGTCTGGCCTCCAACTAAGTCTTGAACAAGGAACTGGTGAAAACTCTTTTCGAAGCCTG ACTTGGCCACCTTCGGGTTCCCCATCTCATGCTGGTACAACCCCACCTGAAAATGGACTCTCAGAGCAC CCCTGTGAAACAGAACAGATAAATGCAAAGAGAAAAGATACAACCAGTGACAAAGATGATTCGCTAGGA AGCCAACAAACAAATGAACAATGTGCTCAAAAGGCTGAGCCAACAGAGTCCTGCGAACAAATTGCTGTC CAAGTGAATAATGGGGATGCTGGAAGGGAGATGCCCTGCCCGTTGCCCTGTGATGAAGAAAGCCCAGAG GCAGAGCTACACAACCATGGAATCCAAATTAATTCCTGTTCTGTGCGACTGGTGGATATAAAAAAGGAA AAGCCATTTTCTAATTCAAAAGTTGAGTGCCAAGCCCAAGCAAGAACTCATCATAACCAGGCATCTGAC ATAATAGTCATCAGCAGTGAGGACTCTGAAGGATCCACTGACGTTGATGAGCCCTTAGAAGTCTTCATC TCAGCACCGAGAAGTGAGCCTGTGATCAATAATGACAACCCTTTAGAATCAAATGATGAAAAGGAGGGC CAAGAAGCCACTTGCTCACGACCCCAGATTGTACCAGAGCCCATGGATTTCAGAAAATTATCTACATTC AGAGAAAGTTTTAAGAAAAGAGTGATAGGACAAGACCACGACTTTTCAGAATCCAGTGAGGAGGAGGCG CCCGCAGAAGCCTCGAGCGGGGCACTGAGAAGCAAGCATGGTGAGAAGGCTCCTATGACTTCTAGAAGT ACATCTACTTGGAGAATACCCAGCAGGAAGAGACGTTTCAGCAGTAGTGACTTTTCAGACCTGAGTAAT GGAGAAGAGCTTCAGGAAACCTGCAGCTCATCCCTAAGAAGAGGGTCAGGTAAAGAAGATTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001206702
Insert Size	1443 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001206702.1
RefSeq Size	2015 bp
RefSeq ORF	1443 bp
Locus ID	6672
UniProt ID	P23497
Protein Families	Transcription Factors
MW	53.8 kDa
Gene Summary	This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (4) lacks multiple exons which represent the 3' coding and UTR regions of variant 1. Variant 4 represents use of an alternate transcription termination site resulting in a much shorter protein (isoform 4, also known as SP100A) with a distinct C-terminus.

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