HNRPDL (HNRNPDL) (NM_001207000) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HNRNP; HNRPDL; JKTBP; JKTBP2; laAUF1; LGMD1G; LGMDD3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331729 representing NM_001207000. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGGTCCCGCCCAGGCTTTCCCATGTGCCGCCGCCATTGTTCCCCTCCGCTCCCGCTACTTTAGCC TCCCGCAGCCTCTCCCATTGGCGGCCGCGGCCGCCGCGGCAGCTAGCCCCGCTCCTCCCTTCGCTCGCT CCCAGCTCCGCCCGGCAGGGGGCGCGCCGGGCCCAGCGCCACGTCACCGCCCAGCAGCCCTCCCGATTG GCGGGCGGGGCGGCTATAAAGGGAGGGCGCAGGCGGCGCCCGGATCTCTTCCGCCGCCATTTTAAATCC AGCTCCATACAACGCTCCGCCGCCGCTGCTGCCGCGACCCGGACTGCGCGCCAGCACCCCCCTGCCGAC AGCTCCGTCACTATGGAGGATATGAACGAGTACAGCAATATAGAGGAATTCGCAGAGGGATCCAAGATC AACGCGAGCAAGAATCAGCAGGATGACGGTAAAATGTTTATTGGAGGCTTGAGCTGGGATACAAGCAAA AAAGATCTGACAGAGTACTTGTCTCGATTTGGGGAAGTTGTAGACTGCACAATTAAAACAGATCCAGTC ACTGGGAGATCAAGAGGATTTGGATTTGTGCTTTTCAAAGATGCTGCTAGTGTTGATAAGGTTTTGGAA CTGAAAGAACACAAACTGGATGGCAAATTGATAGATCCCAAAAGGGCCAAAGCTTTAAAAGGGAAAGAA CCTCCCAAAAAGGTTTTTGTGGGTGGATTGAGCCCGGATACTTCTGAAGAACAAATTAAAGAATATTTT GGAGCCTTTGGAGAGATTGAAAATATTGAACTTCCCATGGATACAAAAACAAATGAAAGAAGAGGATTT TGTTTTATCACATATACTGATGAAGAGCCAGTAAAAAAATTGTTAGAAAGCAGATACCATCAAATTGGT TCTGGGAAGTGTGAAATCAAAGTTGCACAACCCAAAGAGGTATATAGGCAGCAACAGCAACAACAAAAA GGTGGAAGAGGTGCTGCAGCTGGTGGACGAGGTGGTACGAGGGGTCGTGGCCGAGGCCAACAGAGCACT TATGGCAAGGCATCTCGAGGGGGTGGCAATCACCAAAACAATTACCAGCCATACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001207000
Insert Size	1092 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001207000.1
RefSeq Size	3986 bp
RefSeq ORF	1092 bp
Locus ID	9987
UniProt ID	O14979
MW	40 kDa
Gene Summary	This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two RRM domains that bind to RNAs. Three alternatively spliced transcript variants have been described for this gene. One of the variants is probably not translated because the transcript is a candidate for nonsense-mediated mRNA decay. The protein isoforms encoded by this gene are similar to its family member HNRPD. [provided by RefSeq, May 2011] Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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