BRDT (NM_001242810) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BRD6; CT9; SPGF21
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331875 representing NM_001242810. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGATTTAAATACAATTAAGAAGCGCTTGGAGAATAAATATTATGCGAAGGCTTCAGAATGTATAGAA GACTTCAATACAATGTTCTCAAATTGTTATTTATATAACAAGCCTGGAGATGACATTGTTCTTATGGCA CAAGCTCTAGAGAAGCTGTTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAAGTTGTGGGTGTT AAGGAAAGAATCAAGAAAGGCACTCAACAGAATATAGCTGTTTCTTCTGCTAAAGAAAAATCATCACCC AGCGCAACAGAAAAAGTATTTAAGCAGCAAGAAATTCCTTCTGTATTTCCTAAGACATCTATTTCTCCC TTGAACGTGGTACAGGGAGCTTCAGTCAACTCCAGTTCACAAACTGCGGCCCAAGTTACAAAAGGTGTG AAGAGGAAAGCAGATACAACAACTCCTGCAACTTCAGCAGTTAAAGCAAGTAGTGAATTTTCTCCAACA TTCACAGAAAAATCAGTGGCACTGCCACCTATAAAAGAAAATATGCCAAAGAATGTTTTGCCAGATTCT CAGCAACAATATAATGTTGTGAAGACTGTTAAAGTAACTGAACAATTAAGGCACTGTAGTGAGATTCTT AAAGAAATGCTTGCAAAGAAACATTTTTCATATGCATGGCCCTTTTATAATCCTGTTGACGTTAATGCT TTGGGACTCCATAACTACTATGACGTTGTCAAAAATCCGATGGATCTTGGAACTATTAAGGAGAAAATG GATAACCAAGAATATAAGGATGCATACAAATTTGCGGCAGATGTTAGATTAATGTTCATGAATTGCTAC AAGTACAATCCTCCAGATCACGAAGTTGTGACAATGGCAAGAATGCTTCAGGATGTTTTCGAAACGCAT TTTTCAAAGATCCCGATTGAACCTGTTGAGAGTATGCCTTTATGTTACATCAAAACAGATATCACAGAA ACCACTGGTAGAGAGAACACTAATGAAGCCTCCTCTGAAGGGAACTCTTCTGATGATTCTGAAGATGAG CGAGTTAAGCGTCTTGCAAAGCTTCAGGAGCAGCTTAAAGCTGTACATCAACAGCTCCAGGTTTTGTCC CAAGTACCTTTCCGTAAGCTAAATAAAAAGAAAGAGAAGTCTAAAAAGGAAAAGAAAAAAGAAAAGGTT AATAACAGCAATGAAAATCCAAGAAAAATGTGTGAGCAAATGAGGCTAAAGGAAAAGTCCAAGAGAAAT CAGCCAAAGAAAAGGAAACAACAGTTCATTGGTCTAAAATCTGAAGATGAAGATAATGCTAAACCTATG AACTATGATGAGAAAAGGCAGTTAAGTCTGAATATAAACAAACTCCCTGGAGATAAACTTGGGCGAGTA GTTCACATAATACAATCAAGAGAGCCTTCTCTGAGCAATTCCAATCCTGATGAGATAGAGATAGACTTT GAAACACTGAAAGCATCAACACTAAGAGAATTAGAAAAATATGTTTCGGCATGTCTAAGAAAGAGACCA TTAAAACCTCCTGCTAAGAAAATAATGATGTCCAAAGAAGAACTTCACTCACAGAAAAAACAGGAATTG GAAAAGCGGTTACTGGATGTTAATAATCAGTTAAATTCTAGAAAACGTCAAACAAAATCTGATAAAACG CAACCATCCAAAGCTGTTGAAAATGTTTCCCGACTGAGTGAGAGCAGCAGCAGCAGCAGCAGCTCATCA GAGTCTGAAAGTAGCAGCAGTGACTTAAGCTCTTCAGACAGCAGTGATTCTGAATCAGAAATGTTCCCT AAGTTTACAGAAGTAAAACCAAATGATTCTCCTTCTAAAGAGAATGTAAAGAAAATGAAGAATGAATGC ATACTGCCTGAAGGAAGAACAGGCGTCACACAGATAGGATATTGTGTGCAAGACACAACCTCTGCCAAT ACTACCCTTGTTCATCAGACCACACCTTCACATGTAATGCCACCAAATCACCACCAATTAGCATTTAAT TATCAAGAATTAGAACATTTACAGACTGTGAAAAACATTTCACCTTTACAAATTCTGCCTCCCTCAGGT GATTCTGAACAGCTCTCAAATGGCATAACTGTGATGCATCCATCTGGTGATAGTGACACAACGATGTTA GAATCTGAATGTCAAGCTCCTGTACAGAAGGATATAAAGATTAAGAATGCAGATTCATGGAAAAGTTTA GGCAAACCAGTGAAACCATCAGGTGTAATGAAATCCTCAGATGAGCTCTTCAACCAATTTAGAAAAGCA GCCATAGAAAAGGAAGTAAAAGCTCGGACACAGGAACTCATACGGAAGCATTTGGAACAAAATACAAAG GAACTAAAAGCATCTCAAGAAAATCAGAGGGATCTTGGGAATGGATTGACTGTAGAATCTTTTTCAAAT AAAATACAAAACAAGTGCTCTGGAGAAGAGCAGAAAGAACATCAGCAGTCATCAGAAGCTCAAGATAAA TCCAAACTCTGGCTTCTCAAAGACCGTGATTTAGCAAGGCAGAAAGAACAAGAGAGGAGGAGGAGAGAA GCAATGGTGGGTACCATTGATATGACCCTTCAAAGTGACATTATGACAATGTTTGAAAACAACTTTGAT TAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242810
Insert Size	2625 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242810.1
RefSeq Size	3717 bp
RefSeq ORF	2625 bp
Locus ID	676
UniProt ID	Q58F21
Protein Families	Protein Kinase, Transcription Factors
MW	99.4 kDa
Gene Summary	BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jun 2011] Transcript Variant: This variant (7) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (d) is shorter at the N-terminus and lacks a short internal segment compared to isoform a.

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