Phospholipase D2 (PLD2) (NM_001243108) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PLD1C
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331947 representing NM_001243108. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGACGGCGACCCCTGAGAGCCTCTTCCCCACTGGGGACGAACTGGACTCCAGCCAGCTCCAGATGGAG TCCGATGAGGTGGACACCCTGAAGGAGGGAGAGGACCCAGCCGACCGGATGCACCCGTTTCTGGCCATC TATGAGCTTCAGTCTCTGAAAGTGCACCCCTTGGTGTTCGCACCTGGGGTCCCTGTCACAGCCCAGGTG GTGGGCACCGAAAGATATACCAGCGGATCCAAGGTGGGAACCTGCACTCTGTATTCTGTCCGCTTGACT CACGGCGACTTTTCCTGGACAACCAAGAAGAAATACCGTCATTTTCAGGAGCTGCATCGGGACCTCCTG AGACACAAAGTCTTGATGAGTCTGCTCCCTCTGGCTCGATTTGCCGTTGCCTATTCTCCAGCCCGAGAT GCAGGCAACAGAGAGATGCCCTCTCTACCCCGGGCAGGTCCTGAGGGCTCCACCAGACATGCAGCCAGC AAACAGAAATACCTGGAGAATTACCTCAACCGTCTCTTGACCATGTCTTTCTATCGCAACTACCATGCC ATGACAGAGTTCCTGGAAGTCAGTCAGCTGTCCTTTATCCCGGACTTGGGCCGCAAAGGACTGGAGGGG ATGATCCGGAAGCGCTCAGGTGGCCACCGTGTTCCTGGCCTCACCTGCTGTGGCCGAGACCAAGTTTGT TATCGCTGGTCCAAGAGGTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGTGCCTCGAGACAGGT GCCATCTCATTTGTTCAGCTCTTTGACCCTGGCTTTGAGGTGCAAGTGGGGAAAAGGAGCACGGAGGCA CGGCACGGCGTGCGGATCGATACCTCCCACAGGTCCTTGATTCTCAAGTGCAGCAGCTACCGGCAGGCA CGGTGGTGGGCCCAAGAGATCACTGAGCTGGCACAGGGCCCAGGCAGAGACTTCCTACAGCTGCACCGG CATGACAGCTACGCCCCACCCCGGCCTGGGACCTTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTT GCTGCTGTGGCAGATGCCATCCTTCGAGCTCAAGAGGAGATTTTCATCACAGACTGGTGGTTGAGTCCT GAGGTTTACCTGAAGCGTCCGGCCCATTCAGATGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCG GAGGAGGGTGTCCGTGTGTCTATTCTGCTGTTTAAAGAAGTGGAATTGGCCTTGGGCATCAACAGTGGC TATAGCAAGAGGGCGCTGATGCTGCTGCACCCCAACATAAAGGTGATGCGTCACCCAGACCAAGTGACG TTGTGGGCCCATCATGAGAAGCTCCTGGTGGTGGACCAAGTGGTAGCATTCCTGGGGGGACTGGACCTT GCCTATGGCCGCTGGGATGACCTGCACTACCGACTGACTGACCTTGGAGACTCCTCTGAATCAGCTGCC TCCCAGCCTCCCACCCCGCGCCCAGACTCACCAGCCACCCCAGACCTCTCTCACAACCAATTCTTCTGG CTGGGCAAGGACTACAGCAATCTTATCACCAAGGACTGGGTGCAGCTGGACCGGCCTTTCGAAGATTTC ATTGACAGGGAGACGACCCCTCGGATGCCATGGCGGGACGTTGGGGTGGTCGTCCATGGCCTACCGGCC CGGGACCTTGCCCGGCACTTCATCCAGCGCTGGAACTTCACCAAGACCACCAAGGCCAAGTACAAGACT CCCACATACCCCTACCTGCTTCCCAAGTCTACCAGCACGGCCAATCAGCTCCCCTTCACACTTCCAGGA GGGCAGTGCACCACCGTACAGGTCTTGCGATCAGTGGACCGCTGGTCAGCAGGGACTCTGGAGAACTCC ATCCTCAATGCCTACCTGCACACCATCAGGGAGAGCCAGCACTTCCTCTACATTGAGAATCAGTTCTTC ATTAGCTGCTCAGATGGGCGGACGGTTCTGAACAAGGTGGGCGATGAGATTGTGGACAGAATCCTGAAG GCCCACAAACAGGGGTGGTGTTACCGAGTCTACGTGCTTTTGCCCTTACTCCCTGGCTTCGAGGGTGAC ATCTCCACGGGCGGTGGCAACTCCATCCAGGCCATTCTGCACTTTACTTACAGGACCCTGTGTCGTGGG GAGTATTCAATCCTGCATCGCCTTAAAGCAGCCATGGGGACAGCATGGCGGGACTATATTTCCATCTGC GGGCTTCGTACACACGGAGAGCTGGGCGGGCACCCCGTCTCGGAGCTCATCTACATCCACAGCAAGGTG CTCATCGCAGATGACCGGACAGTCATCATTGGTTCTGCAAACATCAATGACCGGAGCTTGCTGGGGAAG CGGGACAGTGAGCTGGCCGTGCTGATCGAGGACACAGAGACGGAACCATCCCTCATGAATGGGGCAGAG TATCAGGCGGGCAGTGTGATTCTTGGAGCAAATACCCGGCCAGACTTGGATCTCCGAGACCCCATCTGT GATGACTTCTTCCAGTTGTGGCAAGACATGGCTGAGAGCAACGCCAATATCTATGAGCAGATCTTCCGC TGCCTGCCATCCAATGCCACGCGTTCCCTGCGGACTCTCCGGGAGTACGTGGCCGTGGAGCCCTTGGCC ACGGTCAGTCCCCCCTTGGCTCGGTCTGAGCTCACCCAGGTCCAGGGCCACCTGGTCCACTTCCCCCTC AAGTTCCTAGAGGATGAGTCTTTGCTGCCCCCGCTGGGTAGCAAGGAGGGCATGATCCCCCTAGAAGTG TGGACATAG
Restriction Sites	SgfI-RsrII
ACCN	NM_001243108
Insert Size	2769 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243108.1
RefSeq Size	3481 bp
RefSeq ORF	2769 bp
Locus ID	5338
UniProt ID	O14939
Protein Families	Druggable Genome
Protein Pathways	Endocytosis, Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Metabolic pathways
MW	104.7 kDa
Gene Summary	The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (PLD2B) has the same N- and C-termini but is shorter compared to isoform PLD2A.

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