CLCN3 (NM_001243372) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ClC-3; CLC3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331995 representing NM_001243372. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGTCTGAGCAGCTGTTCCATAGAGGCTACTATAGAAACAGCTACAACAGTATAACAAGTGCAAGT AGTGATGAGGAACTTTTAGATGGAGCAGGTGTTATTATGGACTTTCAAACATCTGAAGATGACAATTTA TTAGATGGTGACACTGCAGTTGGAACTCATTATACAATGACAAATGGAGGCAGCATTAACAGTTCTACA CATTTACTGGATCTTTTGGATGAACCAATTCCAGGTGTTGGTACATATGATGATTTCCATACTATTGAT TGGGTGCGAGAAAAATGTAAAGACAGAGAAAGGCATAGACGGATCAACAGCAAAAAGAAAGAATCAGCA TGGGAAATGACAAAAAGTTTGTATGATGCGTGGTCAGGATGGCTAGTAGTAACACTAACAGGATTGGCA TCAGGGGCACTGGCCGGATTAATAGACATTGCTGCCGATTGGATGACTGACCTAAAGGAGGGCATTTGC CTTAGTGCGTTGTGGTACAACCACGAACAGTGCTGTTGGGGATCTAATGAAACAACATTTGAAGAGAGG GATAAATGTCCACAGTGGAAAACATGGGCAGAATTAATCATAGGTCAAGCAGAGGGTCCTGGTTCTTAT ATCATGAACTACATAATGTACATCTTCTGGGCCTTGAGTTTTGCCTTTCTTGCAGTTTCCCTGGTAAAG GTATTTGCTCCATATGCCTGTGGCTCTGGAATTCCAGAGATTAAAACTATTTTAAGTGGATTCATCATC AGAGGTTACTTGGGAAAATGGACTTTAATGATTAAAACCATCACATTAGTCCTGGCTGTGGCATCAGGT TTGAGTTTAGGAAAAGAAGGTCCCCTGGTACATGTTGCCTGTTGCTGCGGAAATATCTTTTCCTACCTC TTTCCAAAGTATAGCACAAACGAAGCTAAAAAAAGGGAGGTTAGCTATTATTTTCCTCTCAAAACTTTA TGGAGATCATTTTTTGCTGCTTTAGTGGCTGCATTTGTTTTGAGGTCCATCAATCCATTTGGTAACAGC CGTCTGGTCCTTTTTTATGTGGAGTATCATACACCATGGTACCTTTTTGAACTGTTTCCTTTTATTCTT CTAGGGGTATTTGGAGGGCTTTGGGGAGCCTTTTTCATTAGGGCAAATATTGCCTGGTGTCGTCGACGC AAGTCCACGAAATTTGGAAAGTATCCCGTTCTGGAAGTCATTATTGTTGCAGCCATTACTGCTGTGATA GCCTTCCCTAATCCATACACTAGGCTAAACACCAGTGAACTGATCAAAGAGCTTTTTACAGACTGTGGT CCCCTGGAATCCTCTTCTCTTTGTGACTACAGAAATGACATGAATGCCAGTAAAATTGTCGATGACATT CCTGATCGTCCAGCAGGCATTGGAGTATATTCAGCTATATGGCAGTTATGCCTGGCACTCATATTTAAA ATCATAATGACAGTATTCACTTTTGGCATCAAGGTTCCATCAGGCTTGTTCATCCCCAGCATGGCCATT GGAGCGATCGCAGGAAGGATTGTGGGGATTGCGGTGGAGCAGCTTGCCTACTATCACCACGACTGGTTT ATCTTTAAGGAGTGGTGTGAGGTCGGGGCTGATTGCATTACACCTGGCCTTTATGCCATGGTTGGTGCT GCTGCATGCTTAGGTGGTGTGACAAGAATGACTGTCTCCCTGGTGGTTATTGTTTTTGAGCTTACTGGA GGCTTGGAATATATTGTTCCCCTTATGGCTGCAGTCATGACCAGTAAATGGGTTGGAGATGCCTTTGGC AGGGAAGGCATTTATGAAGCACACATCCGATTAAATGGATACCCTTTCTTGGATGCAAAAGAAGAATTC ACTCATACCACCCTGGCTGCTGACGTTATGAGACCTCGAAGGAATGATCCTCCCTTAGCTGTCCTGACA CAGGACAATATGACAGTGGATGATATAGAAAACATGATTAATGAAACCAGCTACAATGGATTTCCTGTC ATAATGTCAAAAGAATCTCAGAGATTAGTGGGATTTGCCCTCAGAAGAGACCTGACAATTGCAATAGAA AGTGCCAGGAAAAAACAAGAAGGTATCGTTGGCAGTTCTCGGGTGTGTTTTGCACAGCACACCCCATCT CTTCCAGCAGAAAGTCCTCGGCCATTGAAGCTTCGAAGCATTCTTGACATGAGCCCTTTTACAGTGACA GACCACACCCCAATGGAGATCGTGGTGGATATTTTCCGAAAGCTGGGACTGAGGCAGTGCCTTGTAACT CACAATGGGCGCCTCCTTGGCATTATAACAAAAAAAGATATCCTCCGGCATATGGCCCAGACGGCAAAC CAAGACCCCGCTTCAATAATGTTCAACTGA
Restriction Sites	AscI-MluI
ACCN	NM_001243372
Insert Size	2376 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243372.1
RefSeq Size	6142 bp
RefSeq ORF	2376 bp
Locus ID	1182
UniProt ID	P51790
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane
MW	88.5 kDa
Gene Summary	This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (a) lacks an in-frame exon in the middle coding region, compared to variant b. The resulting isoform (a) lacks an internal segment, compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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