TOB (TOB1) (NM_001243885) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APRO5; APRO6; PIG49; TOB; TROB; TROB1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332052 representing NM_001243885. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCATAAGTGACCCAGCCTCATCAGTGTCCAGCTCTCCATCGCCTCCTTTTGGTCACTCTGCTGCT GTAAGCCCTACCTTCATGCCCCGGTCCACTCAGCCTTTAACCTTTACCACTGCCACTTTTGCTGCCACC AAGTTCGGCTCTACCAAAATGAAGAATAGTGGCCGTAGCAACAAGGTTGCACGTACTTCTCCCATCAAC CTCGGCTTGAATGTGAATGACCTCTTGAAGCAGAAAGCCATCTCTTCCTCAATGCACTCTCTGTATGGG CTTGGCTTGGGTAGCCAGCAGCAGCCACAGCAACAGCAGCAGCCAGCCCAGCCGCCACCGCCACCACCA CCACCACAGCAGCAACAACAGCAGAAAACCTCTGCTCTTTCTCCTAATGCCAAGGAATTTATTTTTCCT AATATGCAGGGTCAAGGTAGTAGTACCAATGGAATGTTCCCAGGTGACAGCCCCCTTAACCTCAGTCCT CTCCAGTACAGTAATGCCTTTGATGTGTTTGCAGCCTATGGAGGCCTCAATGAGAAATCTTTTGTAGAT GGCTTGAATTTTAGCTTAAATAACATGCAGTATTCTAACCAGCAATTCCAGCCTGTTATGGCTAACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243885
Insert Size	621 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243885.1
RefSeq Size	2056 bp
RefSeq ORF	621 bp
Locus ID	10140
UniProt ID	P50616
Protein Families	Druggable Genome
MW	22 kDa
Gene Summary	This gene encodes a member of the transducer of erbB-2 /B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The encoded protein may function as a tumor suppressor. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.

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