hHR23b (RAD23B) (NM_001244724) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HHR23B; HR23B; P58
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332088 representing NM_001244724. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGACCAAACCCAAAGCAGTGTCCACACCAGCACCAGCTACAACTCAGCAGTCAGCTCCTGCCAGC ACTACAGCAGTTACTTCCTCCACCACCACAACTGTGGCTCAGGCTCCAACCCCTGTCCCTGCCTTGGCC CCCACTTCCACACCTGCATCCATCACTCCAGCATCAGCGACAGCATCTTCTGAACCTGCACCTGCTAGT GCAGCTAAACAAGAGAAGCCTGCAGAAAAGCCAGCAGAGACACCAGTGGCTACTAGCCCAACAGCAACT GACAGTACATCGGGTGATTCTTCTCGGTCAAACCTTTTTGAAGATGCAACGAGTGCACTTGTGACGGGT CAGTCTTACGAGAATATGGTAACTGAGATCATGTCAATGGGCTATGAACGAGAGCAAGTAATTGCAGCC CTGAGAGCCAGTTTCAACAACCCTGACAGAGCAGTGGAGTATCTTTTAATGGGAATCCCTGGAGATAGA GAAAGTCAGGCTGTGGTTGACCCCCCTCAAGCAGCTAGTACTGGGGCTCCTCAGTCTTCAGCAGTGGCT GCAGCTGCAGCAACTACGACAGCAACAACTACAACAACAAGTTCTGGAGGACATCCCCTTGAATTTTTA CGGAATCAGCCTCAGTTTCAACAGATGAGACAAATTATTCAGCAGAATCCTTCCTTGCTTCCAGCGTTA CTACAGCAGATAGGTCGAGAGAATCCTCAATTACTTCAGCAAATTAGCCAACACCAGGAGCATTTTATT CAGATGTTAAATGAACCAGTTCAAGAAGCTGGTGGTCAAGGAGGAGGAGGTGGAGGTGGCAGTGGAGGA ATTGCAGAAGCTGGAAGTGGTCATATGAACTACATTCAAGTAACACCTCAGGAAAAAGAAGCTATAGAA AGGTTAAAGGCATTAGGATTTCCTGAAGGACTTGTGATACAAGCGTATTTTGCTTGTGAGAAGAATGAG AATTTGGCTGCCAATTTTCTTCTACAGCAGAACTTTGATGAAGATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001244724
Insert Size	1014 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001244724.1
RefSeq Size	3842 bp
RefSeq ORF	1014 bp
Locus ID	5887
UniProt ID	P54727
Protein Families	Druggable Genome
Protein Pathways	Nucleotide excision repair
MW	35 kDa
Gene Summary	The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to isoform (1). The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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