Oct-2 (POU2F2) (NM_001247994) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Oct-2; OCT2; OTF2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332120 representing NM_001247994. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTTCACTCCAGCATGGGGGCTCCAGAAATAAGAATGTCTAAGCCCCTGGAGGCCGAGAAGCAAGGT CTGGACTCCCCATCAGAGCACACAGACACCGAAAGAAATGGACCAGACACTAATCATCAGAACCCCCAA AATAAGACCTCCCCATTCTCCGTGTCCCCAACTGGCCCCAGTACAAAGATCAAGGCTGAAGACCCCAGT GGCGATTCAGCCCCAGCAGCACCCCTGCCCCCTCAGCCGGCCCAGCCTCATCTGCCCCAGGCCCAACTC ATGTTGACGGGCAGCCAGCTAGCTGGGGACATACAGCAGCTCCTCCAGCTCCAGCAGCTGGTGCTTGTG CCAGGCCACCACCTCCAGCCACCTGCTCAGTTCCTGCTACCGCAGGCCCAGCAGAGCCAGCCAGGCCTG CTACCGACACCAAATCTATTCCAGCTACCTCAGCAAACCCAGGGAGCTCTTCTGACCTCCCAGCCCCGG GCCGGGCTTCCCACACAGCCCCCCAAATGCTTGGAGCCACCATCCCACCCCGAGGAGCCCAGTGATCTG GAGGAGCTGGAGCAATTCGCCCGCACCTTCAAGCAACGCCGCATCAAGCTGGGCTTCACGCAGGGTGAT GTGGGCCTGGCCATGGGCAAGCTCTACGGCAACGACTTCAGCCAGACGACCATTTCCCGCTTCGAGGCC CTCAACCTGAGCTTCAAGAACATGTGCAAACTCAAGCCCCTCCTGGAGAAGTGGCTCAACGATGCAGAG ACTATGTCTGTGGACTCAAGCCTGCCCAGCCCCAACCAGCTGAGCAGCCCCAGCCTGGGTTTCGACGGC CTGCCCGGCCGGAGACGCAAGAAGAGGACCAGCATCGAGACAAACGTCCGCTTCGCCTTAGAGAAGAGT TTTCTAGCGAACCAGAAGCCTACCTCAGAGGAGATCCTGCTGATCGCCGAGCAGCTGCACATGGAGAAG GAAGTGATCCGCGTCTGGTTCTGCAACCGGCGCCAGAAGGAGAAACGCATCAACCCCTGCAGTGCGGCC CCCATGCTGCCCAGCCCAGGGAAGCCGGCCAGCTACAGCCCCCATATGGTCACACCCCAAGGGGGCGCG GGGACCTTACCGTTGTCCCAAGCTTCCAGCAGTCTGAGCACAACAGCACAAACCCCAGCCCTCAAGGCA GCCACTCGGCTATCGGCTTGTCAGGCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001247994
Insert Size	1203 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001247994.1
RefSeq Size	6768 bp
RefSeq ORF	1203 bp
Locus ID	5452
UniProt ID	P09086
Protein Families	Transcription Factors
MW	43.5 kDa
Gene Summary	The protein encoded by this gene is a homeobox-containing transcription factor of the POU domain family. The encoded protein binds the octamer sequence 5'-ATTTGCAT-3', a common transcription factor binding site in immunoglobulin gene promoters. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (4) uses alternate splice junctions at the 5' ends of two exons compared to variant 1, the 5'-most being in-frame and the 3'-most causing a frameshift. The resulting isoform (4) lacks an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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