NSP3 (SH2D3C) (NM_001252334) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CHAT; NSP3; PRO34088; SHEP1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332186 representing NM_001252334. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCCCTGCTCTGCCTTCTCCTCACAAGGCTGTCGCCCGTCCTGAGCTCCTGCTGGACACTGCAGCC CACTCAGGAAAACTGAGGGCTCCAGACGGTGGAGAGGGAGCAGAATGTGCTGGGCACAATGGTGGCCCA TCTTGGGGAGTGGGGCAGGGCCAAAGCCAAGAGCCTTCCCGGCAGGGAATCCCACAGGCCCCATGGCTG GTGTCCTGGCAGAGACGGGGCTTTCCACCCAGCTCTTTTTGCCCAGGGCCTTTAAGGACTGAGAAGCTC AGAGCAGGCAGGTGCCCGTTGCTTCTCTGCGGTGGGGCTGCTGGAGAGCCAGAGGCTGGCAGCGACTAT GTGAAGTTCTCCAAGGAGAAGTACATCCTGGACTCATCGCCAGAGAAACTCCACAAGGAATTGGAGGAG GAGCTCAAACTCAGCAGCACGGATCTCCGCAGCCATGCCTGGTACCATGGCCGCATCCCCCGAGAGGTC TCGGAGACCTTGGTACAACGCAACGGCGACTTCCTCATCCGGGACTCACTCACCAGCCTGGGCGACTAT GTGCTCACGTGCCGCTGGCGCAACCAGGCCTTGCACTTCAAGATCAACAAGGTGGTGGTGAAGGCAGGC GAGAGCTACACACACATCCAGTACCTGTTTGAGCAGGAGAGCTTTGACCACGTGCCCGCCCTCGTGCGC TATCATGTGGGCAGCCGCAAGGCTGTGTCAGAGCAGAGTGGTGCCATCATCTACTGCCCGGTGAACCGC ACCTTCCCACTGCGCTACCTCGAGGCCAGCTATGGCCTGGGACAGGGGAGTAGCAAGCCTGCTAGCCCC GTCAGCCCCTCAGGCCCCAAGGGCAGCCACATGAAGCGGCGCAGCGTCACCATGACCGATGGGCTCACT GCTGACAAGGTCACCCGCAGCGATGGCTGCCCCACCAGTACGTCGCTGCCCCGCCCTCGGGACTCCATC CGCAGCTGTGCCCTCAGCATGGACCAGATCCCAGACCTGCACTCACCCATGTCGCCCATCTCCGAGAGC CCTAGCTCCCCTGCCTACAGCACTGTAACCCGTGTCCATGCCGCCCCTGCAGCCCCTTCTGCCACAGCA TTGCCTGCCTCCCCTGTCGCCCGCCGTTCCAGTGAGCCCCAGCTGTGTCCCGGAAGTGCCCCAAAGACC CATGGGGAGTCAGACAAGGGCCCCCACACCAGCCCCTCCCACACCCTTGGCAAGGCCTCCCCGTCACCA TCACTCAGCAGCTACAGTGACCCGGACTCTGGCCACTACTGCCAGCTCCAGCCTCCCGTGCGTGGCAGC CGAGAGTGGGCAGCGACTGAGACCTCCAGCCAGCAGGCCAGGAGCTATGGGGAGAGGCTAAAGGAACTG TCAGAAAATGGGGCCCCTGAAGGGGACTGGGGCAAGACCTTCACAGTCCCCATCGTGGAAGTCACTTCT TCCTTCAACCCGGCCACCTTCCAGTCACTACTGATCCCCAGGGATAACCGGCCACTGGAGGTGGGCCTT CTGCGCAAGGTCAAGGAGCTGCTGGCAGAAGTGGATGCCCGGACGCTGGCCCGGCATGTCACCAAGGTG GACTGCCTGGTTGCTAGGATACTGGGCGTTACCAAGGAGATGCAGACCCTAATGGGAGTCCGCTGGGGC ATGGAACTGCTCACCCTCCCCCATGGCCGGCAGCTACGCCTAGACCTGCTGGAAAGGTTCCACACCATG TCCATCATGCTGGCCGTGGACATCCTGGGCTGCACCGGCTCTGCGGAGGAGCGGGCAGCGCTGCTGCAC AAGACCATTCAGCTGGCGGCCGAGCTGCGGGGGACTATGGGCAACATGTTCAGCTTCGCGGCGGTCATG GGTGCCCTGGACATGGCCCAGATTTCTCGGCTGGAGCAGACATGGGTGACCCTGCGGCAGCGACACACA GAGGGTGCCATCCTGTACGAGAAGAAGCTCAAGCCTTTTCTCAAGAGCCTCAACGAGGGCAAAGAAGGC CCGCCGCTGAGCAACACCACGTTTCCTCATGTGCTGCCCCTCATCACCCTGCTGGAGTGTGACTCGGCC CCACCAGAGGGCCCTGAGCCCTGGGGCAGCACGGAGCACGGCGTGGAGGTGGTGCTGGCTCACCTGGAG GCCGCCCGCACAGTGGCACACCACGGAGGCCTGTACCACACCAATGCTGAAGTCAAGCTGCAGGGGTTC CAGGCCCGGCCGGAGCTCCTGGAGGTGTTCAGCACGGAGTTCCAGATGCGCCTTCTCTGGGGCAGTCAG GGTGCCAGCAGCAGCCAGGCCCGGCGCTATGAGAAGTTCGACAAGGTCCTCACTGCCCTGTCCCACAAG CTGGAACCTGCTGTCCGCTCCAGCGAGCTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001252334
Insert Size	2379 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252334.1
RefSeq Size	3063 bp
RefSeq ORF	2379 bp
Locus ID	10044
UniProt ID	Q8N5H7
Protein Families	Druggable Genome
MW	86.4 kDa
Gene Summary	This gene encodes an adaptor protein and member of a cytoplasmic protein family involved in cell migration. The encoded protein contains a putative Src homology 2 (SH2) domain and guanine nucleotide exchange factor-like domain which allows this signaling protein to form a complex with scaffolding protein Crk-associated substrate. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (7) differs in the 5' UTR and 5' coding region and initiates translation from an alternate start codon, compared to variant 1. The encoded isoform (f) has a distinct N-terminus and is shorter than isoform a.

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