PIK3CB (NM_001256045) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	P110BETA; PI3K; PI3KBETA; PIK3C1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332290 representing NM_001256045. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTGAATCCAATGGGAACTGTTCAAACAAATCCATATACTGAAAATGCAACAGCTTTGCATGTTAAA TTTCCAGAGAATAAAAAACAACCTTATTATTACCCTCCCTTCGATAAGAGTCGAGGTGGAAAAAAGTTT CTTCCTGTATTGAAAGAAATCTTGGACAGGGATCCCTTGTCTCAACTGTGTGAAAATGAAATGGATCTT ATTTGGACTTTGCGACAAGACTGCCGAGAGATTTTCCCACAATCACTGCCAAAATTACTGCTGTCAATC AAGTGGAATAAACTTGAGGATGTTGCTCAGCTTCAGGCGCTGCTTCAGATTTGGCCTAAACTGCCCCCC CGGGAGGCCCTAGAGCTTCTGGATTTCAACTATCCAGACCAGTACGTTCGAGAATATGCTGTAGGCTGC CTGCGACAGATGAGTGATGAAGAACTTTCTCAATATCTTTTACAACTGGTGCAAGTGTTAAAATATGAG CCTTTTCTTGATTGTGCCCTCTCTAGATTCCTATTAGAAAGAGCACTTGGTAATCGGAGGATAGGGCAG TTTCTATTTTGGCATCTTAGGTCAGAAGTGCACATTCCTGCTGTCTCAGTACAATTTGGTGTCATCCTT GAAGCATACTGCCGGGGAAGTGTGGGGCACATGAAAGTGCTTTCTAAGCAGGTTGAAGCACTCAATAAG TTAAAAACTTTAAATAGTTTAATCAAACTGAATGCCGTGAAGTTAAACAGAGCCAAAGGGAAGGAGGCC ATGCATACCTGTTTAAAACAGAGTGCTTACCGGGAAGCCCTCTCTGACCTGCAGTCACCCCTGAACCCA TGTGTTATCCTCTCAGAACTCTATGTTGAAAAGTGCAAATACATGGATTCCAAAATGAAGCCTTTGTGG CTGGTATACAATAACAAGGTATTTGGTGAGGATTCAGTTGGAGTGATTTTTAAAAATGGTGATGATTTA CGACAGGATATGTTGACACTCCAAATGTTGCGCTTGATGGATTTACTCTGGAAAGAAGCTGGTTTGGAT CTTCGGATGTTGCCTTATGGCTGTTTAGCAACAGGAGATCGCTCTGGCCTCATTGAAGTTGTGAGCACC TCTGAAACAATTGCTGACATTCAGCTGAACAGTAGCAATGTGGCTGCTGCAGCAGCCTTCAACAAAGAT GCCCTTCTGAACTGGCTTAAAGAATACAACTCTGGGGATGACCTGGACCGAGCCATTGAGGAATTTACA CTGTCCTGTGCTGGCTACTGTGTAGCTTCTTATGTCCTTGGGATTGGTGACAGACATAGTGACAACATC ATGGTCAAAAAAACTGGCCAGCTCTTCCACATTGACTTTGGACATATTCTTGGAAATTTCAAATCTAAG TTTGGCATTAAAAGGGAGCGAGTGCCTTTTATTCTTACCTATGATTTCATCCATGTCATTCAACAAGGA AAAACAGGAAATACAGAAAAGTTTGGCCGGTTCCGCCAGTGTTGTGAGGATGCATATCTGATTTTACGA CGGCATGGGAATCTCTTCATCACTCTCTTTGCGCTGATGTTGACTGCAGGGCTTCCTGAACTCACATCA GTCAAAGATATACAGTATCTTAAGGACTCTCTTGCATTAGGGAAGAGTGAAGAAGAAGCACTCAAACAG TTTAAGCAAAAATTTGATGAGGCGCTCAGGGAAAGCTGGACTACTAAAGTGAACTGGATGGCCCACACA GTTCGGAAAGACTACAGATCTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256045
Insert Size	1749 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256045.1
RefSeq Size	4797 bp
RefSeq ORF	1749 bp
Locus ID	5291
Protein Families	Druggable Genome
Protein Pathways	Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Inositol phosphate metabolism, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway
MW	66.8 kDa
Gene Summary	This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream AUG compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.