Flightless I (FLII) (NM_001256265) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLI; Fli1; FLIL
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332327 representing NM_001256265. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGGCCACCGGGGTGCTGCCGTTCGTGCGTGGCGTGGACCTCAGCGGCAACGACTTCAAGGGCGGC TACTTCCCTGAGAATGTCAAGGCCATGACCAGCCTGCGGTGGCTGAAGCTGAACCGCACTGGCCTCTGC TACCTGCCCGAGGAGCTGGCCGCCCTGCAGAAGCTGGAACACTTGTCTGTGAGCCACAACAACCTGACC ACGCTTCATGGGGAGCTGTCCAGCCTGCCATCGCTGCGCGCCATCGTGGCCCGAGCCAACAGTCTGAAG AATTCCGGAGTCCCCGATGACATCTTCAAGCTAGATGATCTCTCAGTCCTGGACTTGAGCCACAACCAG CTGACAGAGTGCCCGCGGGAGCTGGAGAACGCCAAGAACATGCTGGTGCTGAACCTCAGCCACAACAGG CAGCTCCCAGCGATGACGGCCCTGCAGACCCTGCACCTGCGGAGCACCCAGCGCACCCAGAGCAACCTG CCCACCAGCCTGGAGGGTCTGAGCAACCTCGCAGACGTGGATCTGTCCTGCAATGACCTGACACGGGTG CCCGAGTGTCTGTACACCCTCCCCAGCCTGCGCCGCCTCAACCTCAGCAGCAACCAGATCACGGAGCTG TCCCTGTGCATAGACCAGTGGGTGCACGTGGAAACTCTGAACCTGTCCCGAAATCAGCTCACCTCACTG CCCTCAGCCATTTGCAAGCTGAGCAAGCTGAAGAAGCTGTACCTGAATTCCAACAAGCTGGACTTTGAC GGGCTGCCCTCAGGCATTGGCAAGCTCACCAACCTGGAAGAGTTCATGGCTGCCAACAACAACCTGGAG CTGGTCCCTGAAAGTCTCTGCAGGTGCCCAAAGCTGAGGAAACTTGTCCTGAACAAGAACCACCTGGTG ACCCTCCCAGAAGCCATCCATTTCCTGACGGAGATCGAGGTCCTGGATGTGCGGGAGAACCCCAACCTG GTCATGCCGCCCAAGCCCGCAGACCGTGCCGCTGAGTGGTACAACATCGACTTCTCGCTGCAGAACCAG CTGCGGCTAGCGGGTGCCTCTCCTGCTACCGTGGCTGCAGCTGCAGCTGGGAGTGGGCCCAAGGACCCT ATGGCTCGCAAGATGCGACTGCGGAGGCGCAAGGATTCAGCCCAGGATGACCAGGCCAAGCAGGTGCTG AAGGGCATGTCAGATGTTGCCCAGGAGAAGAACAAAAAGCAGGAGGAGAGCGCAGATGCCCGGGCCCCC AGCGGGAAGGTGCGGCGTTGGGACCAGGGCCTGGAGAAGCCCCGCCTTGACTACTCCGAGTTCTTCACG GAGGACGTGGGCCAGCTGCCCGGACTGACCATCTGGCAGATAGAGAACTTCGTGCCTGTGCTGGTGGAG GAAGCCTTCCACGGCAAGTTCTACGAGGCTGACTGCTACATTGTGCTCAAGACCTTTCTGGATGACAGC GGCTCCCTCAACTGGGAGATCTACTACTGGATTGGCGGGGAGGCCACACTCGACAAGAAAGCTTGCTCT GCCATCCACGCTGTCAACTTGCGCAACTACCTGGGTGCTGAGTGCCGCACTGTCCGGGAGGAGATGGGC GATGAGAGCGAGGAGTTCCTGCAGGTGTTTGACAACGACATCTCCTACATTGAGGGTGGAACAGCCAGT GGCTTCTACACTGTGGAAGACACACACTATGTCACCAGGATGTATCGTGTGTATGGGAAAAAGAACATC AAGTTGGAGCCTGTGCCCCTCAAGGGGACCTCTCTGGACCCAAGGTTTGTTTTCCTGCTGGACCGAGGG CTAGACATCTACGTATGGCGGGGGGCCCAGGCCACACTGAGCAGCACCACCAAGGCCAGGCTCTTTGCA GAGAAAATTAACAAGAATGAGCGGAAAGGGAAGGCTGAGATCACACTGCTGGTGCAGGGCCAGGAGCTC CCAGAGTTCTGGGAGGCACTGGGTGGGGAGCCCTCTGAGATCAAGAAGCACGTGCCTGAAGACTTCTGG CCGCCGCAGCCCAAGCTGTACAAGGTGGGCCTGGGCTTGGGCTACCTGGAGCTGCCACAGATCAACTAC AAGCTCTCCGTGGAACATAAGCAGCGTCCCAAGGTGGAGCTGATGCCAAGAATGCGGCTGCTGCAGAGT CTGCTGGACACGCGCTGCGTGTACATTCTGGACTGTTGGTCCGACGTGTTCATCTGGCTCGGCCGCAAG TCCCCGCGCCTGGTGCGCGCTGCCGCCCTCAAGCTGGGTCAGGAGCTGTGCGGGATGCTGCACCGGCCA CGCCATGCCACGGTCAGCCGCAGCCTCGAGGGCACCGAGGCGCAGGTGTTCAAGGCCAAGTTCAAGAAT TGGGACGATGTGTTGACGGTGGACTACACACGCAATGCGGAGGCCGTGCTGCAGAGCCCGGGTCTCTCC GGGAAGGTGAAACGCGACGCCGAGAAGAAAGACCAGATGAAGGCTGACCTCACTGCGCTTTTCCTGCCG CGGCAGCCGCCCATGTCGCTGGCCGAGGCGGAGCAGCTGATGGAGGAGTGGAACGAAGACCTAGACGGC ATGGAGGGTTTCGTGCTGGAGGGCAAGAAGTTTGCGCGGCTGCCGGAAGAGGAGTTTGGCCACTTCTAC ACGCAGGACTGCTACGTCTTCCTCTGCAGGTACTGGGTGCCTGTGGAGTACGAGGAGGAGGAAAAGAAG GAAGACAAGGAGGAGAAGGCCGAGGGCAAAGAAGGCGAGGAAGCAACCGCTGAGGCAGAGGAGAAGCAG CCAGAGGAGGACTTCCAGTGCATCGTGTACTTCTGGCAGGGCCGTGAAGCCTCCAATATGGGCTGGCTC ACCTTCACCTTCAGCCTGCAAAAGAAGTTCGAGAGCCTCTTCCCTGGGAAGCTGGAGGTGGTACGCATG ACGCAGCAGCAGGAGAACCCCAAGTTCCTGTCCCATTTCAAGAGGAAGTTCATCATCCACCGGGGCAAG AGGAAGGCGGTCCAGGGCGCCCAACAGCCCAGCCTCTACCAGATCCGCACCAACGGCAGCGCCCTCTGC ACCCGGTGCATCCAGATCAACACCGACTCCAGCCTCCTCAACTCCGAGTTCTGCTTCATCCTCAAGGTT CCCTTTGAGAGTGAGGACAACCAGGGCATCGTGTATGCCTGGGTGGGCCGGGCATCAGACCCTGACGAA GCCAAGTTGGCAGAAGACATCCTGAACACCATGTTTGACACCTCCTACAGCAAGCAGGTTATCAACGAA GGTGAGGAGCCTGAGAACTTCTTCTGGGTGGGCATTGGGGCACAGAAGCCCTATGATGACGATGCCGAG TACATGAAACACACACGTCTCTTCCGGTGCTCCAACGAGAAGGGCTACTTTGCAGTGACTGAGAAATGC TCCGACTTTTGCCAAGATGACCTGGCAGATGATGACATCATGTTGCTAGACAATGGCCAAGAGGTCTAC ATGTGGGTGGGGACCCAGACTAGCCAGGTGGAGATCAAGCTGAGCCTGAAGGCCTGCCAGGTATATATC CAGCACATGCGGTCCAAGGAACATGAGCGGCCGCGCCGGCTGCGCCTGGTCCGCAAGGGCAATGAGCAG CACGCCTTTACCCGCTGCTTCCACGCCTGGAGCGCCTTCTGCAAGGCCCTGGCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256265
Insert Size	3645 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256265.1
RefSeq Size	4222 bp
RefSeq ORF	3645 bp
Locus ID	2314
UniProt ID	Q13045
Protein Families	ES Cell Differentiation/IPS, Transcription Factors
MW	138.5 kDa
Gene Summary	This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (3) is shorter than isoform 1.

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