Leukotriene A4 hydrolase (LTA4H) (NM_001256644) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332433 representing NM_001256644. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTGCCACAGAGAAATCTTTCTAAAAGACAAGTACCGACCATGCATATCCCAGTTAAGACTCGTCGA CTTCTTGCAGCCTTAAAGGTTTTGGATACAAAGGACCTTACAATAGAAAAAGTAGTGATCAATGGACAA GAAGTCAAATATGCTCTTGGAGAAAGACAAAGTTACAAGGGATCGCCAATGGAAATCTCTCTTCCTATC GCTTTGAGCAAAAATCAAGAAATTGTTATAGAAATTTCTTTTGAGACCTCTCCAAAATCTTCTGCTCTC CAGTGGCTCACTCCTGAACAGACTTCTGGGAAGGAACACCCATATCTCTTTAGTCAGTGCCAGGCCATC CACTGCAGAGCAATCCTTCCTTGTCAGGACACTCCTTCTGTGAAATTAACCTATACTGCAGAGGTGTCT GTCCCTAAAGAACTGGTGGCACTTATGAGTGCTATTCGTGATGGAGAAACACCTGACCCAGAAGACCCA AGCAGGAAAATATACAAATTCATCCAAAAAGTTCCAATACCCTGCTACCTGATTGCTTTAGTTGTTGGA GCTTTAGAAAGCAGGCAAATTGGCCCAAGAACTTTGGTGTGGTCTGAGAAAGAGCAGGTGGAAAAGTCT GCTTATGAGTTTTCTGAGACTGAATCTATGCTTAAAATAGCAGAAGATCTGGGAGGACCGTATGTATGG GGACAGTATGACCTATTGGTCCTGCCACCATCCTTCCCTTATGGTGGCATGGAGAATCCTTGCCTTACT TTTGTAACTCCTACTCTACTGGCAGGCGACAAGTCACTCTCCAATGTCATTGCACATGAAATATCTCAT AGCTGGACAGGGAATCTAGTGACCAACAAAACTTGGGATCACTTTTGGTTAAATGAGGGACATACTGTG TACTTGGAACGCCACATTTGCGGACGATTGTTTGGTGAAAAGTTCAGACATTTTAATGCTCTGGGAGGA TGGGGAGAACTACAGAATTCGGTAAAGACATTTGGGGAGACACATCCTTTCACCAAACTTGTGGTTGAT CTGACAGATATAGACCCTGATGTAGCTTATTCTTCAGTTCCCTATGAGAAGGGCTTTGCTTTACTTTTT TACCTTGAACAACTGCTTGGAGGACCAGAGATTTTCCTAGGATTCTTAAAAGCTTATGTTGAGAAGTTT TCCTATAAGAGCATAACTACTGATGACTGGAAGGATTTCCTGTATTCCTATTTTAAAGATAAGGTTGAT GTTCTCAATCAAGTTGATTGGAATGCCTGGCTCTACTCTCCTGGACTGCCTCCCATAAAGCCCAATTAT GATATGACTCTGACAAATGCTTGTATTGCCTTAAGTCAAAGATGGATTACTGCCAAAGAAGATGATTTA AATTCATTCAATGCCACAGACCTGAAGGATCTCTCTTCTCATCAATTGAATGAGTTTTTAGCACAGACG CTCCAGAGGATGGCTGCGGCTCTGCATTCAATCCAAGTGGGAGGACGCAATTCCTTTGGCGCTAAAGAT GGCAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256644
Insert Size	1527 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256644.1
RefSeq Size	2062 bp
RefSeq ORF	1527 bp
Locus ID	4048
UniProt ID	P09960
Protein Families	Druggable Genome, Protease
Protein Pathways	Arachidonic acid metabolism, Metabolic pathways
MW	57.3 kDa
Gene Summary	The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (3) differs in the 5' UTR, initiates translation at an alternate start codon and lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is shorter and has distinct N- and C-termini, compared to isoform 1.

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