GLT28D1 (ALG13) (NM_001257231) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDG1S; CXorf45; DEE36; EIEE36; GLT28D1; MDS031; TDRD13; YGL047W
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332521 representing NM_001257231. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTTACAGGTGCAGGAAGCTGTTTGGAGACTCTGGAAAAAGGAAAGCCACTCGTAGTGGTTATAAAC GAAAAGTTGATGAACAATCATCAGCTGGAACTGGCAAAGCAGCTACACAAAGAGGGTCATCTCTTCTAT TGTACCTGCAGGGTCCTGACTTGTCCTGGGCAAGCCAAGTCCATTGCTTCTGCTCCTGGGAAGTGCCAA GATTCTGCAGCGCTGACTTCAACTGCCTTTTCAGGCCTAGACTTTGGGCTGCTTTCCGGATACCTGCAT AAGCAAGCCCTTGTTACTGCTACCCATCCTACCTGCACCCTGCTTTTTCCCTCTTGCCACGCTTTTTTT CCTCTCCCTCTTACCCCCACCCTGTACAAAATGCATAAAGGATGGAAAAACTACTGCAGCCAGAAGTCT TTGAATGAGGCATCAATGGATGAATATTTAGGCAGCTTAGGGCTGTTTCGAAAGCTGACTGCCAAGGAT GCCTCTTGCCTCTTTCGGGCCATTTCGGAGCAGTTGTTTTGCAGCCAGGTCCATCATTTGGAAATCAGG AAGGCTTGTGTCTCATATATGAGGGAAAATCAACAAACTTTTGAGTCTTATGTGGAGGGATCTTTTGAG AAATACCTGGAACGGTTGGGAGATCCCAAGGAAAGTGCTGGCCAGCTGGAAATAAGAGCTCTTTCTCTA ATTTATAATCGGGATTTCATTCTTTATCGCTTTCCTGGAAAACCTCCAACTTATGTCACAGATAATGGC TATGAAGACAAGATTCTACTCTGCTACTCAAGTAGTGGTCACTATGATTCTGTGTACTCAAAACAATTT CAGTCAAGTGCAGCTGTTTGTCAGGCTGTATTGTACGAAATTCTCTATAAAGATGTGTTTGTTGTGGAT GAAGAAGAGTTGAAGACTGCGATTAAATTGTTTCGAAGTGGTTCTAAGAAGAACAGAAATAATGCTGTA ACTGGAAGCGAGGATGCCCATACTGATTACAAGAGTTCAAATCAGAATAGGATGGAAGAGTGGGGTGCC TGCTACAATGCTGAAAATATACCAGAGGGCTACAATAAAGGAACAGAAGAAACAAAGTCTCCAGAAAAT CCATCAAAGATGCCCTTCCCCTATAAGGTGCTCAAAGCCCTGGATCCAGAAATCTATCGTAATGTAGAA TTTGATGTTTGGTTGGACAGCAGAAAAGAACTTCAAAAATCTGATTACATGGAGTATGCTGGGAGACAG TACTATTTGGGAGACAAGTGTCAGGTTTGCTTGGAATCAGAAGGAAGATATTATAATGCTCATATCCAG GAAGTTGGAAATGAGAACAATTCAGTAACAGTCTTCATTGAAGAATTGGCGGAAAAGCATGTTGTTCCA CTGGCTAACTTAAAACCAGTTACCCAAGTGATGTCTGTTCCTGCCTGGAATGCTATGCCCAGTCGGAAA GGAAGAGGTTACCAGAAAATGCCTGGGGGTTATGTCCCGGAAATAGTTATATCAGAGATGGACATAAAG CAACAGAAGAAGATGTTCAAGAAAATTCGAGGGAAAGAAGTTTACATGACTATGGCTTACGGCAAGGGA GACCCCCTCCTCCCACCCAGGCTGCAGCACAGTATGCATTATGGGCACGATCCTCCAATGCACTACTCA CAGACAGCTGGCAATGTTATGTCTAATGAACATTTTCATCCTCAGCATCCATCTCCGAGACAAGGTCGG GGATATGGGATGCCCAGGAATTCATCTCGGTTTATAAACAGGCACAACATGCCGGGCCCTAAAGTTGAT TTTTACCCAGGCCCAGGTAAAAGGTGCTGCCAGAGCTATGATAACTTCTCTTATAGATCTCGTTCATTT AGACGTAGTCACCGCCAGATGAGTTGTGTGAATAAGGAGTCCCAGTATGGATTTACCCCAGGGAATGGA CAGATGCCCAGGGGCTTGGAAGAAACTATTACTTTTTATGAAGTTGAAGAAGGGGATGAGACTGCTTAT CCAACTTTACCTAATCATGGAGGTCCCTCTACAATGGTTCCTGCTACTTCAGGATACTGTGTTGGAAGG CGGGGACATAGCTCAGGCAAACAGACTTTGAATTTAGAGGAGGGCAATGGCCAGAGTGAAAATGGGCGA TATCATGAAGAATATCTTTATCGTGCAGAGCCAGACTATGAAACTTCAGGTGTTTATAGCACAACTGCA TCTACAGCAAACTTGTCTCTTCAGGACAGAAAGTCATGTTCTATGTCTCCTCAGGACACAGTTACCTCA TACAACTACCCCCAGAAGATGATGGGAAATATTGCAGCAGTTGCAGCTTCCTGTGCCAATAATGTTCCA GCTCCAGTCTTATCTAACGGTGCAGCGGCTAATCAAGCTATTAGTACCACTTCAGTTTCCTCACAGAAT GCTATACAGCCTCTCTTTGTATCTCCACCTACACACGGCAGGCCAGTGATTGCCTCACCATCCTATCCA TGCCATTCTGCTATTCCTCATGCTGGTGCCTCTCTACCACCACCACCACCACCACCACCACCACCACCA CCACCACCACCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTGCTCTTGATGTGGGAGAGACT TCAAACTTACAACCACCACCACCACTACCACCTCCACCTTATTCCTGTGATCCAAGCGGCAGTGATTTG CCTCAAGATACAAAAGTTTTGCAGTACTATTTCAATCTAGGATTGCAGTGCTATTACCACAGCTACTGG CACTCCATGGTCTATGTGCCACAGATGCAGCAGCAGCTTCATGTAGAGAATTATCCAGTCTATACTGAG CCACCTCTGGTAGATCAAACCGTTCCTCAATGCTACAGTGAGGTGAGGAGAGAAGATGGCATACAGGCG GAAGCATCAGCAAATGATACTTTTCCGAATGCTGATTCTTCATCTGTCCCTCATGGAGCAGTCTATTAT CCAGTAATGTCAGATCCCTATGGGCAGCCACCTTTGCCAGGTTTTGACTCCTGCCTTCCGGTTGTGCCA GATTATTCCTGTGTTCCCCCCTGGCATCCAGTTGGTACAGCATATGGTGGTTCTTCTCAAATTCATGGT GCTATAAATCCTGGGCCAATTGGCTGTATTGCTCCATCTCCCCCAGCTTCTCATTATGTACCTCAGGGT ATGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001257231
Insert Size	3180 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001257231.1
RefSeq Size	4193 bp
RefSeq ORF	3180 bp
Locus ID	79868
UniProt ID	Q9NP73
Protein Pathways	Metabolic pathways, N-Glycan biosynthesis
MW	117.5 kDa
Gene Summary	The protein encoded by this gene is a subunit of a bipartite UDP-N-acetylglucosamine transferase. It heterodimerizes with asparagine-linked glycosylation 14 homolog to form a functional UDP-GlcNAc glycosyltransferase that catalyzes the second sugar addition of the highly conserved oligosaccharide precursor in endoplasmic reticulum N-linked glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009] Transcript Variant: This variant (7) differs in the 5' UTR, initiates translation at an alternate start codon and uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (6) is shorter and has a distinct N-terminus, compared to isoform 1.

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