DIAPH3 (NM_001258370) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AN; AUNA1; DIA2; diap3; DRF3; mDia2; NSDAN
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332675 representing NM_001258370. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGTGAGGAGAGGAGCCTTTCCTTATTGGCCAAAGCCGTGGATCCCAGACACCCCAATATGATGACA GATGTGGTTAAACTTCTCTCTGCGGTATGCATTGTAGGGGAAGAAAGCATCCTTGAAGAAGTTTTAGAA GCTTTAACTTCAGCTGGTGAAGAAAAAAAAATTGACAGATTTTTTTGTATTGTGGAAGGCCTCCGGCAC AATTCAGTTCAACTGCAAGTAGCTTGTATGCAGCTCATCAATGCCCTGGTTACATCTCCTGATGATTTG GATTTCAGGCTTCACATCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAATTTAAAA TGCATTAAGAATGATGGCCTGGATATCCAACTTAAAGTCTTTGATGAGCATAAAGAAGAAGATTTGTTT GAGTTATCCCATCGCCTTGAAGATATTAGAGCTGAACTTGATGAAGCATATGATGTTTACAACATGGTG TGGAGCACAGTTAAAGAAACTAGAGCAGAGGGATATTTTATTTCTATTCTTCAGCATCTTTTGCTGATT CGAAATGATTATTTTATAAGGCAACAATACTTCAAATTAATTGATGAGTGTGTATCCCAGATTGTATTG CATAGAGATGGAATGGATCCAGACTTCACATATCGAAAAAGACTAGATTTAGATTTAACCCAGTTTGTA GACATTTGCATAGATCAAGCAAAACTAGAAGAGTTTGAAGAGAAAGCATCAGAACTTTACAAGAAATTT GAAAAAGAGTTTACCGACCACCAAGAAACTCAGGCTGAATTGCAGAAAAAAGAGGCAAAGATTAATGAG CTTCAAGCAGAGCTACAAGCTTTTAAGTCTCAGTTTGGTGCCTTGCCAGCTGATTGTAATATTCCTTTG CCTCCCTCTAAAGAAGGTGGAACTGGCCACTCAGCACTTCCTCCTCCGCCTCCACTGCCTTCTGGTGGA GGGGTGCCGCCTCCACCTCCTCCCCCACCACCTCCTCCACTTCCAGGAATGCGGATGCCATTCAGTGGT CCTGTGCCTCCACCACCTCCCCTGGGATTCCTTGGAGGACAAAATTCTCCTCCTCTACCAATCCTGCCA TTTGGGTTGAAACCAAAGAAAGAATTTAAACCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATC AGACCTCATGAAATGACTGAAAACTGTTTCTGGATAAAAGTAAATGAAAATAAGTATGAAAACGTGGAT TTGCTTTGTAAACTTGAGAATACATTTTGTTGCCAACAAAAAGAGAGAAGAGAAGAGGAAGATATTGAA GAGAAGAAATCGATTAAGAAAAAAATTAAAGAACTTAAGTTTTTAGATTCTAAAATTGCCCAGAACCTT TCAATCTTCCTGAGCTCTTTTCGGGTGCCATATGAGGAAATCAGAATGATGATATTGGAAGTAGATGAA ACACGGTTGGCAGAGTCTATGATTCAGAACTTAATAAAGCATCTTCCTGATCAAGAGCAATTAAATTCA TTGTCTCAGTTCAAGAGTGAATATAGCAACTTATGTGAACCTGAGCAGTTTGTGGTTGTGATGAGCAAT GTGAAGAGACTACGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAGTTTGAAGAGCAGGTGAACAAC ATCAAACCTGACATCATGGCTGTCAGTACTGCCTGCGAAGAGATAAAGAAGAGCAAAAGCTTTAGCAAG TTGCTGGAACTTGTATTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGA TTTAACCTTAGCTCTCTCTGTAAACTAAAGGACACAAAATCAGCAGATCAGAAAACAACGCTACTTCAT TTCCTGGTAGAAATATGTGAAGAGAAGTACCCTGATATACTGAATTTTGTGGATGATTTGGAACCTTTA GACAAAGCTAGTAAAGGTTTGTGCCTTTTTAAGAAACATTTCATGGCTCTGATCTTCAGTGCTAAAAGA CTGAAAATTATACCTTTTATTTGTATGTACTTTCCACTTTCTCACAGTGTTTTCATACCCAATATCTCA TTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258370
Insert Size	2076 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258370.1
RefSeq Size	2300 bp
RefSeq ORF	2076 bp
Locus ID	81624
UniProt ID	Q9NSV4
Protein Pathways	Regulation of actin cytoskeleton
MW	79.4 kDa
Gene Summary	This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (7) differs in the 5' and 3' UTR, lacks a portion of the 5' and 3' coding regions, and initiates translation at an alternate start codon compared to variant 1. The resulting protein (isoform g) has shorter N- and C- termini compared to isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.