Lipin 1 (LPIN1) (NM_001261429) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PAP1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332776 representing NM_001261429. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGCAGAGTGCAGACCATGAATTACGTGGGGCAGTTAGCCGGCCAGGTGTTTGTCACCGTGAAGGAG CTCTACAAGGGGCTGAATCCCGCCACACTCTCAGGGTGCATTGACATCATTGTCATCCGCCAGCCCAAT GGAAACCTCCAATGCTCCCCTTTCCACGTCCGCTTTGGGAAGATGGGGGTCCTGCGCTCCCGAGAGAAA GTGGTTGACATAGAAATCAATGGGGAATCTGTGGATTTGCATATGAAATTGGGAGATAATGGAGAAGCA TTTTTTGTTCAAGAAACAGATAATGATCAGGAAGTTATCCCTATGCACCTGGCCACCTCCCCCATCCTG TCAGAAGGAGCTTCGAGAATGGAATGCCAGCTGAAAAGGGGCTCTGTGGACAGGATGAGAGGCCTGGAC CCCAGCACGCCAGCCCAAGTGATCGCTCCCAGCGAGACGCCGTCAAGCAGCTCTGTAGTAAAGAAGAGA AGAAAAAGGAGGAGAAAGTCACAGCTGGACAGCCTGAAGAGAGATGACAACATGAACACATCTGAGGAT GAGGACATGTTCCCCATCGAGATGAGCTCGGATGAGGCCATGGAGCTGCTGGAGAGCAGCAGAACTCTT CCTAATGATATACCTCCATTCCAAGATGATATTCCTGAGGAAAACCTCTCCCTGGCTGTGATTTACCCT CAGTCAGCCTCATACCCTAATTCGGATAGAGAGTGGTCACCCACTCCCAGTAGCCTGGTAGATTGCAAA AGGACTGCCCCTCATCTTGCAGTTGCGGCCGAGGGAGGTCTGTCTAGTTCTTGCCCTCCACAGTCTTCC CTGTTCCATCCTTCGGAAAGTCCTTCCGGTTCCCGACCTTCAACACCTAAAAGTGATTCAGAATTGGTC AGCAAGTCCACGGAAAGGACAGGGCAGAAGAACCCAGAAATGCTTTGGCTGTGGGGAGAGCTGCCGCAG GCTGCTAAGTCTTCTTCTCCACACAAGATGAAAGAGTCCAGCCCATTGAGCAGTAGAAAAATTTGTGAT AAAAGTCACTTTCAGGCCATTCACAGCGAATCTTCAGACACTTTTAGTGACCAATCGCCAACTCTGGTC GGTGGGGCACTTTTGGACCAGAACAAGCCTCAGACAGAAATGCAGTTTGTGAATGAAGAAGACCTGGAG ACCTTAGGAGCAGCAGCGCCACTCTTGCCCATGATCGAGGAGCTCAAACCCCCCTCTGCCAGTGTAGTC CAGACAGCAAACAAGACGGATTCTCCTTCCAGGAAAAGAGATAAACGAAGCCGACATCTTGGTGCTGAC GGCGTCTACTTGGATGACCTCACAGACATGGATCCTGAAGTGGCGGCCCTGTATTTTCCCAAAAAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001261429
Insert Size	1380 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001261429.1
RefSeq Size	2147 bp
RefSeq ORF	1380 bp
Locus ID	23175
UniProt ID	Q14693
MW	50.5 kDa
Gene Summary	This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (4) has multiple differences, compared to variant 1. These differences result in an isoform (4) with a slightly longer N-terminus and a greatly truncated C-terminus, compared to isoform 1.

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