APOBEC3B (NM_001270411) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	A3B; APOBEC1L; ARCD3; ARP4; bK150C2.2; DJ742C19.2; PHRBNL
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332926 representing NM_001270411. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAATCCACAGATCAGAAATCCGATGGAGCGGATGTATCGAGACACATTCTACGACAACTTTGAAAAC GAACCCATCCTCTATGGTCGGAGCTACACTTGGCTGTGCTATGAAGTGAAAATAAAGAGGGGCCGCTCA AATCTCCTTTGGGACACAGGGGTCTTTCGAGGCCAGGTGTATTTCAAGCCTCAGTACCACGCAGAAATG TGCTTCCTCTCTTGGTTCTGTGGCAACCAGCTGCCTGCTTACAAGTGTTTCCAGATCACCTGGTTTGTA TCCTGGACCCCCTGCCCGGACTGTGTGGCGAAGCTGGCCGAATTCCTGTCTGAGCACCCCAATGTCACC CTGACCATCTCTGCCGCCCGCCTCTACTACTACTGGGAAAGAGATTACCGAAGGGCGCTCTGCAGGCTG AGTCAGGCAGGAGCCCGCGTGAAGATCATGGACTATGAAGAATTTGCATACTGCTGGGAAAACTTTGTG TACAATGAAGGTCAGCAATTCATGCCTTGGTACAAATTCGATGAAAATTATGCATTCCTGCACCGCACG CTAAAGGAGATTCTCAGATACCTGATGGATCCAGACACATTCACTTTCAACTTTAATAATGACCCTTTG GTCCTTCGACGGCGCCAGACCTACTTGTGCTATGAGGTGGAGCGCCTGGACAATGGCACCTGGGTCCTG ATGGACCAGCACATGGGCTTTCTATGCAACGAGTTGGACCCGGCCCAGATCTACAGGGTCACTTGGTTC ATCTCCTGGAGCCCCTGCTTCTCCTGGGGCTGTGCCGGGGAAGTGCGTGCGTTCCTTCAGGAGAACACA CACGTGAGACTGCGCATCTTCGCTGCCCGCATCTATGATTACGACCCCCTATATAAGGAGGCGCTGCAA ATGCTGCGGGATGCTGGGGCCCAAGTCTCCATCATGACCTACGATGAGTTTGAGTACTGCTGGGACACC TTTGTGTACCGCCAGGGATGTCCCTTCCAGCCCTGGGATGGACTAGAGGAGCACAGCCAAGCCCTGAGT GGGAGGCTGCGGGCCATTCTCCAGAATCAGGGAAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270411
Insert Size	1074 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270411.1
RefSeq Size	1485 bp
RefSeq ORF	1074 bp
Locus ID	9582
UniProt ID	Q9UH17
MW	43.1 kDa
Gene Summary	This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of a coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.

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