CSNK1G3 (NM_001270574) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CKI-gamma 3; CSNK1G3L
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332980 representing NM_001270574. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGCTGGAACTGCTGGGACCTAGTTTGGAAGACTTGTTTGACTTGTGTGACAGAACATTTTCTCTT AAAACAGTTCTCATGATAGCTATACAACTGATTTCTCGCATGGAATATGTCCATTCAAAGAACTTGATA TACAGAGATGTAAAACCTGAGAACTTCTTAATAGGACGACCAGGAAACAAAACCCAGCAAGTTATTCAC ATTATAGATTTTGGTTTGGCAAAGGAATATATTGATCCGGAGACAAAGAAACACATACCATACAGAGAA CACAAGAGCCTTACAGGAACAGCTAGATATATGAGCATAAACACACATTTAGGAAAAGAACAAAGTAGA AGAGACGATTTAGAAGCTTTAGGTCATATGTTCATGTATTTTCTGAGAGGCAGTCTTCCTTGGCAAGGC TTAAAGGCTGACACATTAAAGGAGAGGTATCAGAAAATTGGAGATACAAAACGGGCTACACCAATAGAA GTGTTATGTGAAAATTTTCCAGAAGAAATGGCAACATATCTTCGTTATGTAAGAAGGCTAGATTTTTTT GAAAAACCAGACTATGACTACTTAAGAAAGCTTTTTACTGACTTGTTTGATCGAAAAGGATATATGTTT GATTATGAATATGACTGGATTGGTAAACAGTTGCCTACTCCAGTGGGTGCAGTTCAGCAAGATCCTGCT CTGTCATCAAACAGAGAAGCACATCAACACAGAGATAAGATGCAACAATCCAAAAACCAGGTTGTAAGT TCTACAAATGGAGAGTTAAACACAGATGACCCCACCGCAGGACGTTCAAATGCACCCATCACAGCCCCT ACTGAAGTAGAAGTGATGGATGAAACCAACTGCCAGAAAGTGTTGAACATGTGGTGCTGCTGTTTTTTC AAACGAAGGAAAAGGAAAACCATACAGCGCCACAAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270574
Insert Size	936 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270574.1
RefSeq Size	4118 bp
RefSeq ORF	936 bp
Locus ID	1456
UniProt ID	Q9Y6M4
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Hedgehog signaling pathway
MW	36.5 kDa
Gene Summary	This gene encodes a member of a family of serine/threonine protein kinases that phosphorylate caseins and other acidic proteins. A related protein in the African clawed frog participates in the transmission of Wnt/beta-catenin signaling. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (7) differs in the 5' UTR and has multiple differences in the coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (7) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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