Dicer (DICER1) (NM_001271282) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; MNG1; RMSE2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333043 representing NM_001271282. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAAAGCCCTGCTTTGCAACCCCTCAGCATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCA ATGGGTCCTTTCTTTGGACTGCCATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAA TATCAGGTTGAACTGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAGGG AAGACATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACTTCAGCAGAAAT GGAAAAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAACAAGTGTCAGCTGTCAGAACT CATTCAGATCTCAAGGTTGGGGAATACTCAAACCTAGAAGTAAATGCATCTTGGACAAAAGAGAGATGG AACCAAGAGTTTACTAAGCACCAGGTTCTCATTATGACTTGCTATGTCGCCTTGAATGTTTTGAAAAAT GGTTACTTATCACTGTCAGACATTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCAC CCCTATCGAGAAATTATGAAGCTCTGTGAAAATTGTCCATCATGTCCTCGCATTTTGGGACTAACTGCT TCCATTTTAAATGGGAAATGTGATCCAGAGGAATTGGAAGAAAAGATTCAGAAACTAGAGAAAATTCTT AAGAGTAATGCTGAAACTGCAACTGACCTGGTGGTCTTAGACAGGTATACTTCTCAGCCATGTGAGATT GTGGTGGATTGTGGACCATTTACTGACAGAAGTGGGCTTTATGAAAGACTGCTGATGGAATTAGAAGAA GCACTTAATTTTATCAATGATTGTAATATATCTGTACATTCAAAAGAAAGAGATTCTACTTTAATTTCG AAACAGATACTATCAGACTGTCGTGCCGTATTGGTAGTTCTGGGACCCTGGTGTGCAGATAAAGTAGCT GGAATGATGGTAAGAGAACTACAGAAATACATCAAACATGAGCAAGAGGAGCTGCACAGGAAATTTTTA TTGTTTACAGACACTTTCCTAAGGAAAATACATGCACTATGTGAAGAGCACTTCTCACCTGCCTCACTT GACCTGAAATTTGTAACTCCTAAAGTAATCAAACTGCTCGAAATCTTACGCAAATATAAACCATATGAG CGACAGCAGTTTGAAAGCGTTGAGTGGTATAATAATAGAAATCAGGATAATTATGTGTCATGGAGTGAT TCTGAGGATGATGATGAGGATGAAGAAATTGAAGAAAAAGAGAAGCCAGAGACAAATTTTCCTTCTCCT TTTACCAACATTTTGTGCGGAATTATTTTTGTGGAAAGAAGATACACAGCAGTTGTCTTAAACAGATTG ATAAAGGAAGCTGGCAAACAAGATCCAGAGCTGGCTTATATCAGTAGCAATTTCATAACTGGACATGGC ATTGGGAAGAATCAGCCTCGCAACAAACAGATGGAAGCAGAATTCAGAAAACAGGAAGAGGTACTTAGG AAATTTCGAGCACATGAGACCAACCTGCTTATTGCAACAAGTATTGTAGAAGAGGGTGTTGATATACCA AAATGCAACTTGGTGGTTCGTTTTGATTTGCCCACAGAATATCGATCCTATGTTCAATCTAAAGGAAGA GCAAGGGCACCCATCTCTAATTATATAATGTTAGCGGATACAGACAAAATAAAAAGTTTTGAAGAAGAC CTTAAAACCTACAAAGCTATTGAAAAGATCTTGAGAAACAAGTGTTCCAAGTCGGTTGATACTGGTGAG ACTGACATTGATCCTGTCATGGATGATGATGACGTTTTCCCACCATATGTGTTGAGGCCTGACGATGGT GGTCCACGAGTCACAATCAACACGGCCATTGGACACATCAATAGATACTGTGCTAGATTACCAAGTGAT CCGTTTACTCATCTAGCTCCTAAATGCAGAACCCGAGAGTTGCCTGATGGTACATTTTATTCAACTCTT TATCTGCCAATTAACTCACCTCTTCGAGCCTCCATTGTTGGTCCACCAATGAGCTGTGTACGATTGGCT GAAAGAGTTGTAGCTCTCATTTGCTGTGAGAAACTGCACAAAATTGGCGAACTGGATGACCATTTGATG CCAGTTGGGAAAGAGACTGTTAAATATGAAGAGGAGCTTGATTTGCATGATGAAGAAGAGACCAGTGTT CCAGGAAGACCAGGTTCCACGAAACGAAGGCAGTGCTACCCAAAAGCAATTCCAGAGTGTTTGAGGGAT AGTTATCCCAGACCTGATCAGCCCTGTTACCTGTATGTGATAGGAATGGTTTTAACTACACCTTTACCT GATGAACTCAACTTTAGAAGGCGGAAGCTCTATCCTCCTGAAGATACCACAAGATGCTTTGGAATACTG ACGGCCAAACCCATACCTCAGATTCCACACTTTCCTGTGTACACACGCTCTGGAGAGGTTACCATATCC ATTGAGTTGAAGAAGTCTGGTTTCATGTTGTCTCTACAAATGCTTGAGTTGATTACAAGACTTCACCAG TATATATTCTCACATATTCTTCGGCTTGAAAAACCTGCACTAGAATTTAAACCTACAGACGCTGATTCA GCATACTGTGTTCTACCTCTTAATGTTGTTAATGACTCCAGCACTTTGGATATTGACTTTAAATTCATG GAAGATATTGAGAAGTCTGAAGCTCGCATAGGCATTCCCAGTACAAAGTATACAAAAGAAACACCCTTT GTTTTTAAATTAGAAGATTACCAAGATGCCGTTATCATTCCAAGATATCGCAATTTTGATCAGCCTCAT CGATTTTATGTAGCTGATGTGTACACTGATCTTACCCCACTCAGTAAATTTCCTTCCCCTGAGTATGAA ACTTTTGCAGAATATTATAAAACAAAGTACAACCTTGACCTAACCAATCTCAACCAGCCACTGCTGGAT GTGGACCACACATCTTCAAGACTTAATCTTTTGACACCTCGACATTTGAATCAGAAGGGGAAAGCGCTT CCTTTAAGCAGTGCTGAGAAGAGGAAAGCCAAATGGGAAAGTCTGCAGAATAAACAGATACTGGTTCCA GAACTCTGTGCTATACATCCAATTCCAGCATCACTGTGGAGAAAAGCTGTTTGTCTCCCCAGCATACTT TATCGCCTTCACTGCCTTTTGACTGCAGAGGAGCTAAGAGCCCAGACTGCCAGCGATGCTGGCGTGGGA GTCAGATCACTTCCTGCGGATTTTAGATACCCTAACTTAGACTTCGGGTGGAAAAAATCTATTGACAGC AAATCTTTCATCTCAATTTCTAACTCCTCTTCAGCTGAAAATGATAATTACTGTAAGCACAGCACAATT GTCCCTGAAAATGCTGCACATCAAGGTGCTAATAGAACCTCCTCTCTAGAAAATCATGACCAAATGTCT GTGAACTGCAGAACGTTGCTCAGCGAGTCCCCTGGTAAGCTCCACGTTGAAGTTTCAGCAGATCTTACA GCAATTAATGGTCTTTCTTACAATCAAAATCTCGCCAATGGCAGTTATGATTTAGCTAACAGAGACTTT TGCCAAGGAAATCAGCTAAATTACTACAAGCAGGAAATACCCGTGCAACCAACTACCTCATATTCCATT CAGAATTTATACAGTTACGAGAACCAGCCCCAGCCCAGCGATGAATGTACTCTCCTGAGTAATAAATAC CTTGATGGAAATGCTAACAAATCTACCTCAGATGGAAGTCCTGTGATGGCCGTAATGCCTGGTACGACA GACACTATTCAAGTGCTCAAGGGCAGGATGGATTCTGAGCAGAGCCCTTCTATTGGGTACTCCTCAAGG ACTCTTGGCCCCAATCCTGGACTTATTCTTCAGGCTTTGACTCTGTCAAACGCTAGTGATGGATTTAAC CTGGAGCGGCTTGAAATGCTTGGCGACTCCTTTTTAAAGCATGCCATCACCACATATCTATTTTGCACT TACCCTGATGCGCATGAGGGCCGCCTTTCATATATGAGAAGCAAAAAGGTCAGCAACTGTAATCTGTAT CGCCTTGGAAAAAAGAAGGGACTACCCAGCCGCATGGTGGTGTCAATATTTGATCCCCCTGTGAATTGG CTTCCTCCTGGTTATGTAGTAAATCAAGACAAAAGCAACACAGATAAATGGGAAAAAGATGAAATGACA AAAGACTGCATGCTGGCGAATGGCAAACTGGATGAGGATTACGAGGAGGAGGATGAGGAGGAGGAGAGC CTGATGTGGAGGGCTCCGAAGGAAGAGGCTGACTATGAAGATGATTTCCTGGAGTATGATCAGGAACAT ATCAGATTTATAGATAATATGTTAATGGGGTCAGGAGCTTTTGTAAAGAAAATCTCTCTTTCTCCTTTT TCAACCACTGATTCTGCATATGAATGGAAAATGCCCAAAAAATCCTCCTTAGGTAGTATGCCATTTTCA TCAGATTTTGAGGATTTTGACTACAGCTCTTGGGATGCAATGTGCTATCTGGATCCTAGCAAAGCTGTT GAAGAAGATGACTTTGTGGTGGGGTTCTGGAATCCATCAGAAGAAAACTGTGGTGTTGACACGGGAAAG CAGTCCATTTCTTACGACTTGCACACTGAGCAGTGTATTGCTGACAAAAGCATAGCGGACTGTGTGGAA GCCCTGCTGGGCTGCTATTTAACCAGCTGTGGGGAGAGGGCTGCTCAGCTTTTCCTCTGTTCACTGGGG CTGAAGGTGCTCCCGGTAATTAAAAGGACTGATCGGGAAAAGGCCCTGTGCCCTACTCGGGAGAATTTC AACAGCCAACAAAAGAACCTTTCAGTGAGCTGTGCTGCTGCTTCTGTGGCCAGTTCACGCTCTTCTGTA TTGAAAGACTCGGAATATGGTTGTTTGAAGATTCCACCAAGATGTATGTTTGATCATCCAGATGCAGAT AAAACACTGAATCACCTTATATCGGGGTTTGAAAATTTTGAAAAGAAAATCAACTACAGATTCAAGAAT AAGGCTTACCTTCTCCAGGCTTTTACACATGCCTCCTACCACTACAATACTATCACTGATTGTTACCAG CGCTTAGAATTCCTGGGAGATGCGATTTTGGACTACCTCATAACCAAGCACCTTTATGAAGACCCGCGG CAGCACTCCCCGGGGGTCCTGACAGACCTGCGGTCTGCCCTGGTCAACAACACCATCTTTGCATCGCTG GCTGTAAAGTACGACTACCACAAGTACTTCAAAGCTGTCTCTCCTGAGCTCTTCCATGTCATTGATGAC TTTGTGCAGTTTCAGCTTGAGAAGAATGAAATGCAAGGAATGGATTCTGAGCTTAGGAGATCTGAGGAG GATGAAGAGAAAGAAGAGGATATTGAAGTTCCAAAGGCCATGGGGGATATTTTTGAGTCGCTTGCTGGT GCCATTTACATGGATAGTGGGATGTCACTGGAGACAGTCTGGCAGGTGTACTATCCCATGATGCGGCCA CTAATAGAAAAGTTTTCTGCAAATGTACCCCGTTCCCCTGTGCGAGAATTGCTTGAAATGGAACCAGAA ACTGCCAAATTTAGCCCGGCTGAGAGAACTTACGACGGGAAGGTCAGAGTCACTGTGGAAGTAGTAGGA AAGGGGAAATTTAAAGGTGTTGGTCGAAGTTACAGGATTGCCAAATCTGCAGCAGCAAGAAGAGCCCTC CGAAGCCTCAAAGCTAATCAACCTCAGGTTCCCAATAGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271282
Insert Size	5769 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271282.1
RefSeq Size	6192 bp
RefSeq ORF	5769 bp
Locus ID	23405
UniProt ID	Q9UPY3
Protein Families	Druggable Genome
MW	218.7 kDa
Gene Summary	This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010] Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 2. Variants 1, 2, 4 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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