KCNMA1 (NM_001271518) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA205K10.1; BKTM; CADEDS; hSlo; IEG16; KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA; SLO1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333059 representing NM_001271518. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCAAATGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGAGGCAGCAGTCTT AGAATGAGTAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCCTCCTCCTCT TCCTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTC ATCATCCCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCCTGGCC TCCTCCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGG ACCGTGTGCTGCCACTGCGGGGGCAAGACGAAGGTTGTCTTAGTCTTTGCTCTCAGCATCGGTGCACTT GTAATATACTTCATAGATTCATCAAACCCAATAGAATCCTGCCAGAATTTCTACAAAGATTTCACATTA CAGATCGACATGGCTTTCAACGTGTTCTTCCTTCTCTACTTCGGCTTGCGGTTTATTGCAGCCAACGAT AAATTGTGGTTCTGGCTGGAAGTGAACTCTGTAGTGGATTTCTTCACGGTGCCCCCCGTGTTTGTGTCT GTGTACTTAAACAGAAGTTGGCTTGGTTTGAGATTTTTAAGAGCTCTGAGACTGATACAGTTTTCAGAA ATTTTGCAGTTTCTGAATATTCTTAAAACAAGTAATTCCATCAAGCTGGTGAATCTGCTCTCCATATTT ATCAGCACGTGGCTGACTGCAGCCGGGTTCATCCATTTGGTGGAGAATTCAGGGGACCCATGGGAAAAT TTCCAAAACAACCAGGCTCTCACCTACTGGGAATGTGTCTATTTACTCATGGTCACAATGTCCACCGTT GGTTATGGGGATGTTTATGCAAAAACCACACTTGGGCGCCTCTTCATGGTCTTCTTCATCCTCGGGGGA CTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATACGGGGGC TCCTATAGTGCGGTTAGTGGAAGAAAGCACATTGTGGTCTGCGGACACATCACTCTGGAGAGTGTTTCC AACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGACGTCAATGTGGAGATCGTTTTTCTTCACAAC ATCTCCCCCAACCTGGAGCTTGAAGCTCTGTTCAAACGACATTTTACTCAGGTGGAATTTTATCAGGGT TCCGTCCTCAATCCACATGATCTTGCAAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTTGCC AACAAGTACTGCGCTGACCCGGATGCGGAGGATGCCTCGAATATCATGAGAGTAATCTCCATAAAGAAC TACCATCCGAAGATAAGAATCATCACTCAAATGCTGCAGTATCACAACAAGGCCCATCTGCTAAACATC CCGAGCTGGAATTGGAAAGAAGGTGATGACGCAATCTGCCTCGCAGAGTTGAAGTTGGGCTTCATAGCC CAGAGCTGCCTGGCTCAAGGCCTCTCCACCATGCTTGCCAACCTCTTCTCCATGAGGTCATTCATAAAG ATTGAGGAAGACACATGGCAGAAATACTACTTGGAAGGAGTCTCAAATGAAATGTACACAGAATATCTC TCCAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTCAAGCTCCTA ATGATAGCCATTGAGTACAAGTCTGCCAACCGAGAGAGCCGAAGCCGAAAGCGTATATTAATTAATCCT GGAAACCATCTTAAGATCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAA AGGGCATTTTTTTACTGCAAGGCCTGTCATGATGACATCACAGATCCCAAAAGAATAAAAAAATGTGGC TGCAAACGGCTTGAAGATGAGCAGCCGTCAACACTATCACCAAAAAAAAAGCAACGGAATGGAGGCATG CGGAACTCACCCAACACCTCGCCTAAGCTGATGAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAG ATTGACAACATGGACTCCAATGTGAAGAAGTACGACTCTACTGGGATGTTTCACTGGTGTGCACCCAAG GAGATAGAGAAAGTCATCCTGACTCGAAGTGAAGCTGCCATGACCGTCCTGAGTGGCCATGTCGTGGTC TGCATCTTTGGCGACGTCAGCTCAGCCCTGATCGGCCTCCGGAACCTGGTGATGCCGCTCCGTGCCAGC AACTTTCATTACCATGAGCTCAAGCACATTGTGTTTGTGGGCTCTATTGAGTACCTCAAGCGGGAATGG GAGACGCTTCATAACTTCCCCAAAGTGTCCATATTGCCTGGTACGCCATTAAGTCGGGCTGATTTAAGG GCTGTCAACATCAACCTCTGTGACATGTGCGTTATCCTGTCAGCCAATCAGAATAATATTGATGATACT TCGCTGCAGGACAAGGAATGCATCTTGGCGTCACTCAACATCAAATCTATGCAGTTTGATGACAGCATC GGAGTCTTGCAGGCTAATTCCCAAGGGTTCACACCTCCAGGAATGGATAGATCCTCTCCAGATAACAGC CCAGTGCACGGGATGTTACGTCAACCATCCATCACAACTGGGGTCAACATCCCCATCATCACTGAACTA GTGAACGATACTAATGTTCAGTTTTTGGACCAAGACGATGATGATGACCCTGATACAGAACTGTACCTC ACGCAGCCCTTTGCCTGTGGGACAGCATTTGCCGTCAGTGTCCTGGACTCACTCATGAGCGCGACGTAC TTCAATGACAATATCCTCACCCTGATACGGACCCTGGTGACCGGAGGAGCCACGCCGGAGCTGGAGGCT CTGATTGCTGAGGAAAACGCCCTTAGAGGTGGCTACAGCACCCCGCAGACACTGGCCAATAGGGACCGC TGCCGCGTGGCCCAGTTAGCTCTGCTCGATGGGCCATTTGCGGACTTAGGGGATGGTGGTTGTTATGGT GATCTGTTCTGCAAAGCTCTGAAAACATATAATATGCTTTGTTTTGGAATTTACCGGCTGAGAGATGCT CACCTCAGCACCCCCAGTCAGTGCACAAAGAGGTATGTCATCACCAACCCGCCCTATGAGTTTGAGCTC GTGCCGACGGACCTGATCTTCTGCTTAATGCAGTTTGACCACAATGCCGGCCAGTCCCGGGCCAGCCTG TCCCATTCCTCCCACTCGTCGCAGTCCTCCAGCAAGAAGAGCTCCTCTGTTCACTCCATCCCATCCACA GCAAACCGACAGAACCGGCCCAAGTCCAGGGAGTCCCGGGACAAACAGAACAGAAAAGAAATGGTTTAC AGATGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001271518
Insert Size	3387 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271518.1
RefSeq Size	11832 bp
RefSeq ORF	3387 bp
Locus ID	3778
UniProt ID	Q12791
Protein Families	Druggable Genome, Ion Channels: Potassium, Transmembrane
Protein Pathways	Vascular smooth muscle contraction
MW	125.9 kDa
Gene Summary	MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (5) omits an in-frame coding exon. The resulting isoform (e) is shorter compared to isoform a.

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