PPP1R12C (NM_001271618) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AAVS1; LENG3; MBS85; p84; p85
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333073 representing NM_001271618. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCCGGAGAGGATGGCCCGGCGGCTGGCCCGGGGGCGGCGGCGGCGGCTGCCCGGGAGCGGCGACGG GAGCAGCTGCGGCAGTGGGGGGCGCGGGCGGGCGCCGAGCCTGGCCCCGGAGAGCGCCGCGCCCGCACC GTCCGCTTCGAGCGCGCCGCCGAGTTCCTGGCGGCCTGTGCGGGCGGCGACCTGGACGAGGCGCGTCTG ATGCTGCGCGCCGCCGACCCTGGCCCCGGCGCCGAGCTCGACCCCGCCGCGCCGCCGCCCGCCCGCGCC GTGCTGGACTCCACCAACGCCGACGGTATCAGCGCCCTGCACCAGGCCTGCATTGATGAGAACCTGGAG GTGGTGCGCTTCTTGGTGGAGCAGGGCGCCACTGTGAACCAGGCAGACAACGAGGGCTGGACGCCACTG CACGTGGCCGCCTCCTGTGGCTACCTAGATATCGCCAGGTACCTCCTGAGCCACGGGGCCAACATCGCC GCCGTCAACAGTGACGGGGACCTGCCCCTGGACCTGGCCGAGTCGGACGCCATGGAGGGGCTGCTGAAG GCGGAGATCGCCCGCCGAGGTGTGGATGTGGAAGCAGCCAAGCGGGCAGAAGAGGAATTGCTCCTTCAT GACACGAGGTGCTGGCTGAATGGGGGCGCCATGCCAGAGGCCCGGCACCCCCGCACAGGCGCCTCTGCC CTGCACGTGGCTGCTGCCAAGGGCTACATTGAGGTGATGAGGTTGCTCCTTCAGGCTGGCTACGACCCA GAGCTCCGGGACGGGGACGGCTGGACTCCCCTGCACGCAGCGGCACACTGGGGCGTGGAGGATGCCTGC CGCCTGCTGGCCGAGCATGGCGGGGGCATGGACTCACTGACCCATGCGGGGCAGCGTCCCTGTGACCTG GCCGATGAGGAAGTACTGAGCCTGTTGGAGGAACTGGCCCGGAAACAGGAGGACCTTCGGAACCAAAAA GAAGCTTCCCAGAGCCGGGGCCAGGAGCCCCAAGCGCCCTCTAGCAGCAAACACAGAAGGAGCTCTGTG TGTCGTCTGAGCAGTCGCGAGAAGATTTCCCTCCAGGACTTGTCCAAGGAGCGCCGGCCTGGTGGGGCT GGGGGGCCCCCCATCCAGGACGAGGATGAGGGGGAAGAAGGTCCCACCGAACCACCCCCTGCAGAACCC AGAACCCTCAATGGCGTCTCCTCCCCGCCGCACCCCAGCCCTAAGAGTCCCGTGCTTGAAGAGGCCCCC TTCTCCAGGCGCTTTGGCCTCCTGAAGACAGGGAGTTCTGGTGCCCTGGGTCCCCCTGAAAGGCGGACA GCGGAGGGAGCCCCTGGGGCTGGGCTGCAGCGCTCGGCTTCCTCCTCCTGGCTGGAAGGGACCTCCACT CAGGCCAAGGAGCTCCGTCTTGCCAGAATTACCCCGACCCCCTCCCCGAAGCTGCCGGAGCCCTCTGTC CTGTCTGAGGTCACCAAGCCTCCTCCTTGCTTGGAGAACTCCTCGCCTCCCTCCAGGATTCCGGAGCCT GAATCCCCAGCGAAGCCAAACGTCCCCACAGCCTCCACGGCGCCCCCAGCGGACTCCCGGGACCGACGG AGGTCCTACCAGATGCCTGTGCGGGATGAGGAGTCGGAATCCCAGAGAAAAGCTCGCTCCCGTCTCATG CGCCAGTCTCGGAGGTCCACACAGGGTGTGACTCTTACAGACCTGAAGGAGGCAGAGAAGGCTGCAGGG AAGGCCCCAGAGTCAGAGAAGCCGGCGCAGAGCCTGGACCCTTCCCGAAGGCCCCGCGTCCCTGGAGTG GAGAACTCTGACAGCCCTGCCCAGAGAGAGGCGCCCGACGGGCAGGGTCCGGGACCGCAGGCGGCCAGG GAGCACCGCAAGGTCGGAAAGGAGTGGAGGGGGCCTGCGGAGGGGGAGGAGGCGGAGCCGGCTGACCGC AGCCAGGAGTCCAGCACCCTGGAGGGCGGCCCCTCGGCCCGCAGGCAGCGGTGGCAGCGGGACCTCAAC CCAGAACCTGAGCCAGAATCGGAAGAGCCAGACGGAGGCTTTAGGACGCTGTATGCAGAGCTGCGCAGG GAGAACGAGCGGCTTCGCGAGGCCCTGACCGAGACCACGCTGCGGCTGGCGCAGCTCAAGGTGGAGCTG GAGCGGGCCACGCAGAGGCAAGAACGCTTCGCTGAGAGGCCAGCCCTCCTGGAACTGGAGAGATTCGAG CGCAGGGCCCTGGAACGCAAGGCCGCAGAGCTGGAGGAGGAGCTGAAGGCCCTGTCTGACCTCCGCGCT GACAACCAGCGCCTCAAGGATGAGAATGCAGCGTTGATCCGCGTCATCAGCAAACTCTCCAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271618
Insert Size	2343 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271618.1
RefSeq Size	2959 bp
RefSeq ORF	2343 bp
Locus ID	54776
UniProt ID	Q9BZL4
Protein Families	Druggable Genome
MW	84.7 kDa
Gene Summary	The gene encodes a subunit of myosin phosphatase. The encoded protein regulates the catalytic activity of protein phosphatase 1 delta and assembly of the actin cytoskeleton. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (2) uses an alternate in-frame splice site. The encoded isoform (b) is shorter compared to isoform a.

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