ZNF423 (NM_001271620) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Ebfaz; hOAZ; JBTS19; NPHP14; OAZ; Roaz; Zfp104; ZFP423
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333074 representing NM_001271620. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGGATGAATCAATTTACACCTGCGATCACTGTCAGCAGGACTTCGAGTCTCTGGCAGACCTGACG GACCACCGGGCCCACCGCTGTCCTGGAGATGGTGATGACGACCCACAACTCTCCTGGGTGGCCTCGTCT CCCTCCAGCAAGGATGTTGCGTCACCCACGCAGATGATCGGAGATGGTTGTGACCTCGGCCTCGGCGAG GAGGAAGGGGGCACGGGCCTGCCATACCCTTGCCAGTTCTGCGACAAGTCCTTCATCCGCTTGAGCTAC TTGAAGAGGCACGAGCAGATCCACAGCGACAAGCTGCCGTTCAAGTGCACCTACTGCAGCCGCCTCTTC AAGCACAAGAGGAGCCGTGACCGGCACATCAAGCTGCATACGGGCGACAAGAAGTATCACTGCCACGAG TGCGAGGCAGCCTTCTCCCGCAGCGACCACCTCAAGATCCACCTGAAGACCCACAGCTCCAGCAAGCCC TTCAAGTGCACTGTGTGCAAGCGCGGCTTCTCCTCCACCAGCTCGCTGCAGAGCCACATGCAGGCCCAC AAAAAGAACAAGGAGCATCTGGCCAAGTCGGAGAAGGAAGCCAAGAAGGACGACTTCATGTGCGACTAC TGCGAGGACACCTTCAGCCAGACGGAGGAGCTGGAGAAGCACGTGCTCACCCGCCACCCGCAGCTGTCC GAGAAGGCGGACCTGCAGTGCATTCACTGCCCTGAGGTCTTCGTCGACGAGAACACACTGCTCGCCCAT ATCCACCAAGCCCACGCCAACCAGAAACACAAGTGCCCCATGTGCCCTGAGCAGTTCTCCTCAGTGGAA GGTGTCTACTGCCACCTGGACAGCCACCGGCAGCCCGACTCCAGCAACCACAGTGTCAGTCCCGACCCT GTACTGGGCAGCGTGGCCTCCATGAGCAGCGCCACACCCGACTCCAGCGCCTCTGTGGAGCGTGGCTCC ACCCCGGACTCCACCTTGAAGCCGCTGCGGGGGCAGAAGAAGATGCGGGATGACGGGCAGGGCTGGACC AAGGTGGTCTATAGCTGCCCCTATTGTTCCAAGCGGGACTTTAACAGCCTGGCCGTGCTGGAGATCCAC CTGAAGACCATCCACGCGGACAAGCCCCAGCAGAGCCACACATGTCAGATCTGCCTGGACTCCATGCCC ACCCTCTACAACCTCAACGAGCACGTTCGCAAGCTGCACAAGAACCATGCCTACCCTGTGATGCAGTTT GGCAACATCTCTGCCTTCCACTGCAACTACTGCCCCGAGATGTTCGCCGACATCAATAGCCTGCAGGAG CACATCCGCGTCTCCCACTGCGGCCCCAACGCCAACCCCTCTGACGGTAATAATGCTTTCTTCTGCAAC CAGTGCTCCATGGGTTTCCTTACTGAGTCCTCCCTCACCGAGCACATCCAGCAGGCCCACTGCAGTGTG GGCAGTGCCAAACTAGAGTCTCCGGTGGTGCAGCCCACGCAGTCCTTCATGGAGGTCTATTCCTGCCCC TACTGCACCAACTCCCCCATCTTTGGCTCCATCCTGAAACTCACCAAGCACATCAAGGAGAACCACAAG AACATTCCACTGGCCCACAGCAAGAAGTCCAAGGCCGAGCAGAGCCCAGTCTCGTCCGATGTGGAGGTG TCTTCCCCGAAGCGGCAGCGGCTCTCAGCAAGCGCCAACTCCATCTCCAATGGGGAGTATCCTTGCAAT CAATGCGACCTCAAGTTCTCCAACTTTGAGAGCTTCCAGACCCACCTGAAGCTGCACCTGGAGCTGCTG CTGCGGAAGCAAGCGTGCCCCCAGTGCAAAGAGGACTTTGACTCCCAGGAGTCCCTCCTGCAGCACCTG ACAGTGCATTACATGACCACGTCGACCCACTATGTGTGCGAGAGCTGCGACAAGCAATTTTCCTCGGTG GATGACCTGCAGAAGCACCTGCTGGACATGCACACCTTTGTGTTGTACCACTGCACCCTGTGTCAGGAG GTCTTCGACTCCAAGGTGTCCATCCAGGTGCACCTGGCGGTGAAGCACAGCAATGAGAAGAAGATGTAC CGCTGCACGGCCTGCAACTGGGACTTCCGCAAGGAGGCTGACCTGCAGGTGCACGTCAAACACAGCCAC CTGGGCAACCCGGCCAAGGCTCACAAGTGCATCTTCTGTGGGGAGACCTTCAGCACCGAGGTGGAGCTG CAGTGCCACATCACCACACACAGCAAGAAGTATAACTGTAAGTTCTGCAGCAAGGCCTTCCACGCCATC ATCCTGCTGGAGAAGCACCTGCGGGAGAAGCACTGTGTGTTTGATGCTGCGACCGAGAACGGCACGGCC AATGGGGTACCCCCAATGGCCACCAAGAAAGCTGAGCCTGCTGACCTGCAGGGCATGCTGCTTAAGAAC CCTGAGGCACCTAACAGCCATGAGGCCAGCGAGGATGACGTGGACGCGTCGGAGCCCATGTACGGCTGT GACATCTGTGGGGCGGCCTACACCATGGAGGTGCTGCTGCAGAATCACCGGCTGCGGGACCACAATATC CGGCCGGGCGAGGATGATGGCTCACGCAAGAAGGCTGAGTTTATCAAGGGCAGTCACAAGTGCAACGTT TGTTCACGGACTTTCTTCTCGGAGAACGGGCTACGGGAGCACCTGCAGACGCACCGGGGCCCTGCCAAG CACTACATGTGTCCCATCTGTGGTGAGCGCTTCCCTTCGCTGCTGACGCTCACCGAACACAAGGTGACC CACAGCAAGAGCCTGGACACGGGCACCTGTCGCATCTGCAAGATGCCCCTGCAGAGCGAGGAGGAGTTT ATTGAGCACTGCCAGATGCACCCTGACCTGCGCAACTCACTCACGGGCTTCCGCTGTGTGGTCTGCATG CAGACAGTCACTTCCACGCTTGAGCTCAAGATCCATGGCACCTTCCACATGCAGAAGCTGGCGGGCAGC TCAGCGGCGTCCTCCCCCAATGGCCAGGGGCTGCAGAAGCTCTACAAGTGCGCCCTGTGCCTCAAGGAG TTCCGCAGCAAGCAGGACCTGGTGAAGCTTGACGTCAATGGGCTGCCCTACGGCCTCTGCGCCGGCTGC ATGGCCCGCAGCGCCAACGGACAGGTGGGTGGCCTGGCCCCGCCCGAGCCCGCCGACCGGCCCTGTGCC GGCCTCCGTTGCCCCGAGTGCAGTGTCAAGTTTGAGAGTGCCGAAGACCTGGAGAGCCACATGCAGGTG GACCACCGTGACCTCACGCCGGAGACCAGTGGGCCCCGGAAAGGCACCCAGACATCGCCAGTGCCCCGG AAAAAGACATACCAGTGCATCAAGTGCCAGATGACCTTCGAGAACGAGAGAGAGATCCAAATCCACGTT GCCAACCACATGATTGAGGAAGGCATCAACCACGAGTGTAAGCTGTGCAACCAGATGTTCGACTCCCCG GCCAAGCTCCTCTGTCACCTCATTGAGCACAGCTTCGAGGGCATGGGCGGCACCTTCAAATGCCCCGTG TGTTTCACAGTCTTCGTCCAGGCCAACAAGTTGCAGCAGCACATCTTTGCCGTGCACGGGCAGGAGGAC AAGATCTACGACTGCTCACAGTGCCCTCAGAAGTTCTTCTTCCAGACCGAGCTGCAGAACCACACGATG AGCCAGCACGCACAGTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001271620
Insert Size	3675 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271620.1
RefSeq Size	4706 bp
RefSeq ORF	3675 bp
Locus ID	23090
UniProt ID	Q2M1K9
MW	138 kDa
Gene Summary	The protein encoded by this gene is a nuclear protein that belongs to the family of Kruppel-like C2H2 zinc finger proteins. It functions as a DNA-binding transcription factor by using distinct zinc fingers in different signaling pathways. Thus, it is thought that this gene may have multiple roles in signal transduction during development. Mutations in this gene are associated with nephronophthisis-14 and Joubert syndrome-19. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1, which results in translation initiation from an in-frame downstream AUG, and an isoform (2) with a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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