SLIT3 (NM_001271946) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MEGF5; SLIL2; Slit-3; SLIT1; slit2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333162 representing NM_001271946. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCCCCGGGTGGGCAGGGGTCGGCGCCGCCGTGCGCGCCCGCCTGGCGCTGGCCTTGGCGCTGGCG AGCGTCCTGAGTGGGCCTCCAGCCGTCGCCTGCCCCACCAAGTGTACCTGCTCCGCTGCCAGCGTGGAC TGCCACGGGCTGGGCCTCCGCGCGGTTCCTCGGGGCATCCCCCGCAACGCTGAGCGCCTTGACCTGGAC AGAAATAATATCACCAGGATCACCAAGATGGACTTCGCTGGGCTCAAGAACCTCCGAGTCTTGCATCTG GAAGACAACCAGGTCAGCGTCATCGAGAGAGGCGCCTTCCAGGACCTGAAGCAGCTAGAGCGACTGCGC CTGAACAAGAATAAGCTGCAAGTCCTTCCAGAATTGCTTTTCCAGAGCACGCCGAAGCTCACCAGACTA GATTTGAGTGAAAACCAGATCCAGGGGATCCCGAGGAAGGCGTTCCGCGGCATCACCGATGTGAAGAAC CTGCAACTGGACAACAACCACATCAGCTGCATTGAAGATGGAGCCTTCCGAGCGCTGCGCGATTTGGAG ATCCTTACCCTCAACAACAACAACATCAGTCGCATCCTGGTCACCAGCTTCAACCACATGCCGAAGATC CGAACTCTGCGCCTCCACTCCAACCACCTGTACTGCGACTGCCACCTGGCCTGGCTCTCGGATTGGCTG CGACAGCGACGGACAGTTGGCCAGTTCACACTCTGCATGGCTCCTGTGCATTTGAGGGGCTTCAACGTG GCGGATGTGCAGAAGAAGGAGTACGTGTGCCCAGCCCCCCACTCGGAGCCCCCATCCTGCAATGCCAAC TCCATCTCCTGCCCTTCGCCCTGCACGTGCAGCAATAACATCGTGGACTGTCGAGGAAAGGGCTTGATG GAGATTCCTGCCAACTTGCCGGAGGGCATCGTCGAAATACGCCTAGAACAGAACTCCATCAAAGCCATC CCTGCAGGAGCCTTCACCCAGTACAAGAAACTGAAGCGAATAGACATCAGCAAGAATCAGATATCGGAT ATTGCTCCAGATGCCTTCCAGGGCCTGAAATCACTCACATCGCTGGTCCTGTATGGGAACAAGATCACC GAGATTGCCAAGGGACTGTTTGATGGGCTGGTGTCCCTACAGCTGCTCCTCCTCAATGCCAACAAGATC AACTGCCTGCGGGTGAACACGTTTCAGGACCTGCAGAACCTCAACTTGCTCTCCCTGTATGACAACAAG CTGCAGACCATCAGCAAGGGGCTCTTCGCCCCTCTGCAGTCCATCCAGACACTCCACTTAGCCCAAAAC CCATTTGTGTGCGACTGCCACTTGAAGTGGCTGGCCGACTACCTCCAGGACAACCCCATCGAGACAAGC GGGGCCCGCTGCAGCAGCCCGCGCCGACTCGCCAACAAGCGCATCAGCCAGATCAAGAGCAAGAAGTTC CGCTGCTCAGGCTCCGAGGATTACCGCAGCAGGTTCAGCAGCGAGTGCTTCATGGACCTCGTGTGCCCC GAGAAGTGTCGCTGTGAGGGCACGATTGTGGACTGCTCCAACCAGAAGCTGGTCCGCATCCCAAGCCAC CTCCCTGAATATGTCACCGACCTGCGACTGAATGACAATGAGGTATCTGTTCTGGAGGCCACTGGCATC TTCAAGAAGTTGCCCAACCTGCGGAAAATAAATCTGAGTAACAATAAGATCAAGGAGGTGCGAGAGGGA GCTTTCGATGGAGCAGCCAGCGTGCAGGAGCTGATGCTGACAGGGAACCAGCTGGAGACCGTGCACGGG CGCGTGTTCCGTGGCCTCAGTGGCCTCAAAACCTTGATGCTGAGGAGTAACTTGATCGGCTGTGTGAGT AATGACACCTTTGCCGGCCTGAGTTCGGTGAGACTGCTGTCCCTCTATGACAATCGGATCACCACCATC ACCCCTGGGGCCTTCACCACGCTTGTCTCCCTGTCCACCATAAACCTCCTGTCCAACCCCTTCAACTGC AACTGCCACCTGGCCTGGCTCGGCAAGTGGTTGAGGAAGAGGCGGATCGTCAGTGGGAACCCTAGGTGC CAGAAGCCATTTTTCCTCAAGGAGATTCCCATCCAGGATGTGGCCATCCAGGACTTCACCTGTGATGGC AACGAGGAGAGTAGCTGCCAGCTGAGCCCGCGCTGCCCGGAGCAGTGCACCTGTATGGAGACAGTGGTG CGATGCAGCAACAAGGGGCTCCGCGCCCTCCCCAGAGGCATGCCCAAGGATGTGACCGAGCTGTACCTG GAAGGAAACCACCTAACAGCCGTGCCCAGAGAGCTGTCCGCCCTCCGACACCTGACGCTTATTGACCTG AGCAACAACAGCATCAGCATGCTGACCAATTACACCTTCAGTAACATGTCTCACCTCTCCACTCTGATC CTGAGCTACAACCGGCTGAGGTGCATCCCCGTCCACGCCTTCAACGGGCTGCGGTCCCTGCGAGTGCTA ACCCTCCATGGCAATGACATTTCCAGCGTTCCTGAAGGCTCCTTCAACGACCTCACATCTCTTTCCCAT CTGGCGCTGGGAACCAACCCACTCCACTGTGACTGCAGTCTTCGGTGGCTGTCGGAGTGGGTGAAGGCG GGGTACAAGGAGCCTGGCATCGCCCGCTGCAGTAGCCCTGAGCCCATGGCTGACAGGCTCCTGCTCACC ACCCCAACCCACCGCTTCCAGTGCAAAGTACTCTGGTTTTGCTGCCCAGGGCCAGTGGACATCAACATT GTGGCCAAATGCAATGCCTGCCTCTCCAGCCCGTGCAAGAATAACGGGACATGCACCCAGGACCCTGTG GAGCTGTACCGCTGTGCCTGCCCCTACAGCTACAAGGGCAAGGACTGCACTGTGCCCATCAACACCTGC ATCCAGAACCCCTGTCAGCATGGAGGCACCTGCCACCTGAGTGACAGCCACAAGGATGGGTTCAGCTGC TCCTGCCCTCTGGGCTTTGAGGGGCAGCGGTGTGAGATCAACCCAGATGACTGTGAGGACAACGACTGC GAAAACAATGCCACCTGCGTGGACGGGATCAACAACTACGTGTGTATCTGTCCGCCTAACTACACAGGT GAGCTATGCGACGAGGTGATTGACCACTGTGTGCCTGAGCTGAACCTCTGTCAGCATGAGGCCAAGTGC ATCCCCCTGGACAAAGGATTCAGCTGCGAGTGTGTCCCTGGCTACAGCGGGAAGCTCTGTGAGACAGAC AATGATGACTGTGTGGCCCACAAGTGCCGCCACGGGGCCCAGTGCGTGGACACAATCAATGGCTACACA TGCACCTGCCCCCAGGGCTTCAGTGGACCCTTCTGTGAACACCCCCCACCCATGGTCCTACTGCAGACC AGCCCATGCGACCAGTACGAGTGCCAGAACGGGGCCCAGTGCATCGTGGTGCAGCAGGAGCCCACCTGC CGCTGCCCACCAGGCTTCGCCGGCCCCAGATGCGAGAAGCTCATCACTGTCAACTTCGTGGGCAAAGAC TCCTACGTGGAACTGGCCTCCGCCAAGGTCCGACCCCAGGCCAACATCTCCCTGCAGGTGGCCACTGAC AAGGACAACGGCATCCTTCTCTACAAAGGAGACAATGACCCCCTGGCACTGGAGCTGTACCAGGGCCAC GTGCGGCTGGTCTATGACAGCCTGAGTTCCCCTCCAACCACAGTGTACAGTGTGGAGACAGTGAATGAT GGGCAGTTTCACAGTGTGGAGCTGGTGACGCTAAACCAGACCCTGAACCTAGTAGTGGACAAAGGAACT CCAAAGAGCCTGGGGAAGCTCCAGAAGCAGCCAGCAGTGGGCATCAACAGCCCCCTCTACCTTGGAGGC ATCCCCACCTCCACCGGCCTCTCTGCCTTGCGCCAGGGCACGGACCGGCCTCTAGGCGGCTTCCACGGA TGCATCCATGAGGTGCGCATCAACAACGAGCTGCAGGACTTCAAGGCCCTCCCACCACAGTCCCTGGGG GTGTCACCAGGCTGCAAGTCCTGCACCGTGTGCAAGCACGGCCTGTGCCGCTCCGTGGAGAAGGACAGC GTGGTGTGCGAGTGCCGCCCAGGCTGGACCGGCCCACTCTGCGATCAGGAGGCCCGGGACCCCTGCCTC GGCCACAGATGCCACCATGGAAAATGTGTGGCAACTGGGACCTCATACATGTGCAAGTGTGCCGAGGGC TATGGAGGGGACTTGTGTGACAACAAGAATGACTCTGCCAATGCCTGCTCAGCCTTCAAGTGTCACCAT GGGCAGTGCCACATCTCAGACCAAGGGGAGCCCTACTGCCTGTGCCAGCCCGGCTTTAGCGGCGAGCAC TGCCAACAAGAGAATCCGTGCCTGGGACAAGTAGTCCGAGAGGTGATCCGCCGCCAGAAAGGTTATGCA TCATGTGCCACAGCCTCCAAGGTGCCCATCATGGAATGTCGTGGGGGCTGTGGGCCCCAGTGCTGCCAG CCCACCCGCAGCAAGCGGCGGAAATACGTCTTCCAGTGCACGGACGGCTCCTCGTTTGTAGAAGAGGTG GAGAGACACTTAGAGTGCGGCTGCCTCGCGTGTTCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271946
Insert Size	4593 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271946.1
RefSeq Size	9748 bp
RefSeq ORF	4593 bp
Locus ID	6586
UniProt ID	O75094
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Axon guidance
MW	168.5 kDa
Gene Summary	The protein encoded by this gene is secreted, likely interacting with roundabout homolog receptors to effect cell migration. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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