ERCC6 (NM_001277059) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3; POF11; RAD26; UVSS1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333269 representing NM_001277059. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCAAATGAGGGAATCCCCCACTCAAGTCAAACTCAGGAGCAAGACTGTTTACAGAGTCAACCTGTC AGTAATAATGAAGAAATGGCAATCAAGCAAGAAAGTGGTGGTGATGGGGAGGTGGAGGAGTACCTCTCC TTTCGTTCTGTGGGTGACGGGCTGTCCACCTCTGCTGTGGGGTGCGCATCAGCAGCTCCGAGGAGAGGG CCAGCCCTGCTGCACATCGACCGACATCAGATCCAGGCAGTAGAGCCTAGCGCCCAGGCCCTTGAGCTG CAGGGTTTGGGTGTGGACGTCTATGACCAGGACGTGCTGGAACAGGGAGTGCTTCAGCAGGTGGACAAT GCCATCCATGAGGCCAGCCGTGCCTCCCAGCTCGTTGACGTGGAGAAGGAGTATCGGTCGGTCCTGGAT GACCTCACGTCATGTACGACATCCCTAAGGCAAATCAATAAAATTATTGAACAGCTTAGCCCTCAAGCT GCCACCAGCAGAGACATCAACAGGAAACTAGATTCTGTAAAACGACAGAAGTATAATAAGGAACAACAG CTAAAAAAGATCACTGCAAAACAAAAGCATCTCCAGGCCATCCTTGGAGGAGCAGAGGTGAAAATTGAA CTAGATCACGCCAGTCTGGAGGAGGATGCAGAGCCGGGGCCATCCAGTCTTGGCAGCATGCTCATGCCT GTCCAGGAGACTGCCTGGGAAGAGCTCATCCGCACTGGCCAGATGACACCTTTTGGTACCCAGATCCCT CAGAAACAGGAGAAAAAGCCCAGAAAAATCATGCTTAATGAAGCATCAGGCTTCGAAAAGTATTTGGCA GATCAAGCAAAACTGTCTTTTGAAAGGAAGAAGCAAGGTTGTAATAAAAGAGCAGCTAGAAAAGCTCCA GCCCCAGTCACGCCTCCAGCCCCAGTGCAAAATAAAAACAAACCAAACAAGAAAGCCAGAGTTCTGTCC AAAAAAGAGGAGCGTTTGAAAAAGCACATCAAGAAACTCCAGAAGAGGGCTTTGCAGTTCCAGGGGAAA GTGGGATTGCCAAAGGCAAGGAGACCTTGGGAGTCAGACATGAGGCCAGAGGCAGAGGGAGACTCTGAG GGTGAAGAGTCTGAGTATTTCCCCACAGAGGAGGAGGAAGAGGAGGAAGATGACGAGGTGGAGGGGGCA GAGGCGGACCTGTCTGGAGATGGTACTGACTATGAGCTGAAGCCTCTGCCCAAGGGCGGGAAACGGCAG AAGAAAGTGCCAGTGCAGGAGATTGATGATGACTTTTTCCCAAGTTCTGGGGAAGAAGCTGAAGCTGCT TCTGTAGGAGAAGGAGGAGGAGGAGGTCGGAAAGTGGGAAGATACCGAGATGATGGAGATGAAGATTAT TATAAGCAGCGGTTAAGTCCCAAGATGCCTCGAACACTAAGTTTACATGAAATAACTGACCTTTTAGAG ACAGATGACAGCATAGAAGCAAGTGCTATAGTGATACAACCACCTGAAAATGCTACAGCACCTGTTTCT GATGAGGAATCAGGAGATGAAGAAGGTGGAACAATAAATAATCTGCCAGGTTCTTTGTTGCACACAGCT GCGTATCTTATTCAAGATGGCTCTGATGCTGAGTCTGACTCAGATGATCCCTCATACGCACCTAAAGAT GACTCTCCTGATGAAGTTCCATCTACGTTTACTGTGCAGCAACCTCCACCATCAAGGAGGAGGAAAATG ACAAAAATTCTTTGCAAATGGAAAAAAGCCGACCTAACTGTACAACCCGTAGCAGGTAGAGTTACAGCA CCACCAAACGATTTCTTCACCGTAATGAGAACTCCCACAGAAATTCTTGAACTTTTTCTTGATGACGAG GTCATTGAACTCATTGTCAAGTACTCCAACTTATATGCTTGCAGTAAAGGTGTACATCTTGGCTTGACT AGCTCTGAATTCAAATGTTTTCTGGGAATTATTTTTCTGAGTGGTTATGTCTCAGTTCCTAGAAGGCGT ATGTTTTGGGAACAAAGAACAGATGTGCATAATGTACTGGTTAGTGCTGCCATGAGACGTGACCGGTTT GAAACTATATTTTCTAATTTGCATGTTGCTGACAATGCAAATTTGGATCCAGTGGACAAATTTTCCAAA TTGCGACCTCTCATAAGCAAACTTAATGAGAGATGCATGAAATTTGTTCCAAATGAAACATATTTCAGC TTTGATGAATTCATGGTTCCTTATTTTGGTCGTCACGGGTGCAAACAATTTATTCGGGGAAAGCCCATT CGGTTTGGCTATAAGTTTTGGTGTGGTGCCACCTGTCTGGGCTACATTTGCTGGTTTCAGCCGTATCAG GGTAAAAACCCAAATACTAAACATGAGGAATATGGTGTCGGTGCGTCACTTGTCCTTCAGTTTAGTGAG GCACTTACAGAGGCACACCCTGGACAATACCATTTTGTATTCAATAACTTTTTCACCAGTATTGCACTT CTTGATAAGCTCAGTTCAATGGGACATCAGGCAACAGGTACAGTGAGAAAGGATCACATTGACAGAGTT CCACTGGAATCAGATGTAGCTTTAAAGAAAAAAGAAAGAGGCACATTTGATTATCGAATTGATGGCAAA GGCAATATTGTCTGCAGATGGAATGATAACAGTGTTGTCACTGTTGCCTCATCTGGTGCTGGTATCCAT CCCCTGTGTCTTGTCAGTCGTTACTCCCAGAAACTGAAAAAGAAGATACAAGTTCAGCAGCCAAACATG ATCAAAGTGTATAACCAGTTCATGGGAGGCGTAGACAGAGCTGATGAAAACATTGATAAGTATCGGGCA TCAATCCGTGGAAAGAAATGGTATTCAAGCCCTCTTTTGTTCTGTTTCGAACTGGTCTTACAAAATGCT TGGCAATTGCATAAAACATATGATGAGAAACCAGTGGATTTTCTGGAGTTTCGTCGACGTGTGGTATGC CATTATCTGGAGACCCATGGTCATCCTCCAGAACCTGGCCAAAAAGGAAGACCTCAGAAGCGTAACATT GACTCACGTTATGATGGCATAAATCATGTGATAGTCAAACAGGGAAAGCAAACGCGATGCGCTGAATGT CATAAGAACACAACTTTTCGATGTGAAAAATGTGATGTTGCCTTACATGTGAAGTGTTCCGTTGAATAT CACACTGAATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001277059
Insert Size	3186 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277059.1
RefSeq Size	3535 bp
RefSeq ORF	3186 bp
Locus ID	2074
UniProt ID	P0DP91
Protein Families	Druggable Genome
Protein Pathways	Nucleotide excision repair
MW	119.5 kDa
Gene Summary	This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The encoded protein has ATP-stimulated ATPase activity, interacts with several transcription and excision repair proteins, and may promote complex formation at DNA repair sites. Mutations in this gene are associated with Cockayne syndrome type B and cerebrooculofacioskeletal syndrome 1. Alternative splicing occurs between a splice site from exon 5 of this gene to the 3' splice site upstream of the open reading frame (ORF) of the adjacent gene, piggyback-derived-3 (GeneID:267004), which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (2) differs in the 5' UTR and contains an alternate 3' terminal exon, compared to variant 3. It is generated as a result of splicing of ERCC6 exon 5 to the 3' splice site upstream of the PGBD3 ORF that activates the alternative polyadenylation site downstream of the PGBD3 ORF. The resulting protein (isoform 1, ERCC6-PGBD3 fusion protein) is shorter and has a distinct C-terminus, compared to isoform 2.

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