SHANK3 (NM_033517) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DEL22q13.3; PROSAP2; PSAP2; SCZD15; SPANK-2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333299 representing NM_033517. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGACGGCCCCGGGGCCAGCGCCGTGGTCGTGCGCGTCGGCATCCCGGACCTGCAGCAGACGAAGTGC CTGCGCCTGGACCCGGCCGCGCCCGTGTGGGCCGCCAAGCAGCGCGTGCTCTGCGCCCTCAACCACAGC CTCCAGGACGCGCTCAACTATGGGCTTTTCCAGCCGCCCTCCCGGGGCCGCGCCGGCAAGTTCCTGGAT GAGGAGCGGCTCCTGCAGGAGTACCCGCCCAACCTGGACACGCCCCTGCCCTACCTGGAGTTTCGATAC AAGCGGCGAGTTTATGCCCAGAACCTCATCGATGATAAGCAGTTTGCAAAGCTTCACACAAAGGCGAAC CTGAAGAAGTTCATGGACTACGTCCAGCTGCATAGCACGGACAAGGTGGCACGCCTGTTGGACAAGGGG CTGGACCCCAACTTCCATGACCCTGACTCAGGAGAGTGCCCCCTGAGCCTCGCAGCCCAGCTGGACAAC GCCACGGACCTGCTAAAGGTGCTGAAGAATGGTGGTGCCCACCTGGACTTCCGCACTCGCGATGGGCTC ACTGCCGTGCACTGTGCCACACGCCAGCGGAATGCGGCAGCACTGACGACCCTGCTGGACCTGGGGGCT TCACCTGACTACAAGGACAGCCGCGGCTTGACACCCCTCTACCACAGCGCCCTGGGGGGTGGGGATGCC CTCTGCTGTGAGCTGCTTCTCCACGACCACGCTCAGCTGGGGATCACCGACGAGAATGGCTGGCAGGAG ATCCACCAGGCCTGCCGCTTTGGGCACGTGCAGCATCTGGAGCACCTGCTGTTCTATGGGGCAGACATG GGGGCCCAGAACGCCTCGGGGAACACAGCCCTGCACATCTGTGCCCTCTACAACCAGGAGAGCTGTGCT CGTGTCCTGCTCTTCCGTGGAGCTAACAGGGATGTCCGCAACTACAACAGCCAGACAGCCTTCCAGGTG GCCATCATCGCAGGGAACTTTGAGCTTGCAGAGGTTATCAAGACCCACAAAGACTCGGATGTTGTACCA TTCAGGGAAACCCCCAGCTATGCGAAGCGGCGGCGACTGGCTGGCCCCAGTGGCTTGGCATCCCCTCGG CCTCTGCAGCGCTCAGCCAGCGATATCAACCTGAAGGGGGAGGCACAGCCAGCAGCTTCTCCTGGACCC TCGCTGAGAAGCCTCCCCCACCAGCTGCTGCTCCAGCGGCTGCAAGAGGAGAAAGATCGTGACCGGGAT GCCGACCAGGAGAGCAACATCAGTGGCCCTTTAGCAGGCAGGGCCGGCCAAAGCAAGATCAGCCCGAGC GGGCCCGGCGGCCCCGGCCCCGCGCCCGGCCCCGGCCCCGCGCCCCCTGCGCCCCCCGCACCGCCGCCC CGGGGCCCGAAGCGGAAACTTTACAGCGCCGTCCCCGGCCGCAAGTTCATCGCCGTGAAGGCGCACAGC CCGCAGGGTGAAGGCGAGATCCCGCTGCACCGCGGCGAGGCCGTGAAGGTGCTCAGCATTGGGGAGGGC GGTTTCTGGGAGGGAACCGTGAAAGGCCGCACGGGCTGGTTCCCGGCCGACTGCGTGGAGGAAGTGCAG ATGAGGCAGCATGACACACGGCCTGAAACGCGGGAGGACCGGACGAAGCGGCTCTTTCGGCACTACACA GTGGGCTCCTACGACAGCCTCACCTCACACAGCGATTATGTCATTGATGACAAAGTGGCTGTCCTGCAG AAACGGGACCACGAGGGCTTTGGTTTTGTGCTCCGGGGAGCCAAAGCAGAGACCCCCATCGAGGAGTTC ACGCCCACGCCAGCCTTCCCGGCGCTGCAGTATCTCGAGTCGGTGGACGTGGAGGGTGTGGCCTGGAGG GCCGGGCTGCGCACGGGAGACTTCCTCATCGAGGTGAACGGGGTGAACGTGGTGAAGGTCGGACACAAG CAGGTGGTGGCTCTGATTCGCCAGGGTGGCAACCGCCTCGTCATGAAGGTTGTGTCTGTGACAAGGAAG CCAGAAGAGGACGGGGCTCGGCGCAGAGCCCCACCGCCCCCCAAGAGGGCCCCCAGCACCACACTGACC CTGCGCTCCAAGTCCATGACAGCTGAGCTCGAGGAACTTGCCTCCATTCGGAGAAGAAAAGGGGAGAAG CTGGACGAGATGCTGGCAGCCGCCGCAGAGCCAACGCTGCGGCCAGACATCGCAGACGCAGACTCCAGA GCCGCCACCGTCAAACAGAGGCCCACCAGTCGGAGGATCACACCCGCCGAGATTAGCTCATTGTTTGAA CGCCAGGGCCTCCCAGGCCCAGAGAAGCTGCCGGGCTCCTTGCGGAAGGGGATTCCACGGACCAAGTCT GTAGGGGAGGACGAGAAGCTGGCGTCCCTGCTGGAAGGGCGCTTCCCGCGGAGCACCTCGATGCAAGAC CCGGTGCGCGAGGGTCGCGGCATCCCGCCCCCGCCGCAGACCGCGCCGCCTCCCCCGCCCGCGCCCTAC TACTTCGACTCGGGGCCGCCCCCGGCCTTCTCGCCGCCGCCCCCGCCGGGCCGCGCCTACGACACGGTG CGCTCCAGCTTCAAGCCCGGCCTGGAGGCGCGCCTGGGCGCGGGCGCTGCCGGCCTGTACGAGCCGGGC GCGGCCCTCGGCCCGCTGCCGTATCCCGAGCGGCAGAAGCGCGCGCGCTCCATGATCATCCTGCAGGAC TCGGCGCCCGAGTCGGGCGACGCCCCTCGACCCCCGCCCGCGGCCACCCCGCCCGAGCGACCCAAGCGC CGGCCGCGGCCGCCCGGCCCCGACAGCCCCTACGCCAACCTGGGCGCCTTCAGCGCCAGCCTCTTCGCT CCGTCCAAGCCGCAGCGCCGCAAGAGCCCCCTGGTGAAGCAGCTGCAGGTGGAGGACGCGCAGGAGCGC GCGGCCCTGGCCGTGGGCAGCCCCGGTCCCGGCGGCGGCAGCTTCGCCCGCGAGCCCTCCCCGACCCAC CGCGGTCCGCGCCCGGGTGGCCTCGACTACGGCGCGGGCGATGGCCCGGGGCTCGCGTTCGGCGGCCCG GGCCCGGCCAAGGACCGGCGGCTGGAGGAGCGGCGCCGCTCCACTGTGTTCCTGTCCGTGGGGGCCATC GAGGGCAGCGCCCCCGGCGCGGATCTGCCATCCCTACAGCCCTCCCGCTCCATCGACGAGCGCCTCCTG GGGACCGGCCCCACCGCCGGCCGCGACCTGCTGCTGCCCTCCCCGGTGTCTGCCCTGAAGCCGTTGGTC AGCGGCCCGAGCCTGGGGCCCTCGGGTTCCACCTTCATCCACCCACTCACCGGCAAACCCCTGGACCCC AGCTCACCCCTGGCCCTTGCCCTGGCTGCCCGAGAGCGAGCTCTGGCCTCCCAGGCGCCCTCCCGGTCC CCCACACCCGTGCACAGTCCCGACGCCGACCGCCCCGGACCCCTGTTTGTGGATGTACAGGCCCGGGAC CCAGAGCGAGGGTCCCTGGCTTCCCCGGCTTTCTCCCCACGGAGCCCAGCCTGGATTCCTGTGCCTGCT CGCAGGGAGGCAGAGAAGGTCCCCCGGGAGGAGCGGAAGTCACCCGAGGACAAGAAGTCCATGATCCTC AGCGTCCTGGACACATCCCTGCAGCGGCCAGCTGGCCTCATCGTTGTGCACGCCACCAGCAACGGGCAG GAGCCCAGCAGGCTGGGGGGGGCCGAAGAGGAGCGCCCGGGCACCCCGGAGTTGGCCCCGGCCCCCATG CAGTCAGCGGCTGTGGCAGAGCCCCTGCCCAGCCCCCGGGCCCAGCCCCCTGGTGGCACCCCGGCAGAC GCCGGGCCAGGCCAGGGCAGCTCAGAGGAAGAGCCAGAGCTGGTGTTTGCTGTGAACCTGCCACCTGCC CAGCTGTCGTCCAGCGATGAGGAGACCAGGGAGGAGCTGGCCCGAATTGGGTTGGTGCCACCCCCTGAA GAGTTTGCCAACGGGGTCCTGCTGGCCACCCCACTCGCTGGCCCGGGCCCCTCGCCCACCACGGTGCCC AGCCCGGCCTCAGGGAAGCCCAGCAGTGAGCCACCCCCTGCCCCTGAGTCTGCAGCCGACTCTGGGGTG GAGGAGGCTGACACACGCAGCTCCAGCGACCCCCACCTGGAGACCACAAGCACCATCTCCACGGTGTCC AGCATGTCCACCTTGAGCTCGGAGAGCGGGGAACTCACTGACACCCACACCTCCTTCGCTGACGGACAC ACTTTTCTACTCGAGAAGCCACCAGTGCCTCCCAAGCCCAAGCTCAAGTCCCCGCTGGGGAAGGGGCCG GTGACCTTCAGGGACCCGCTGCTGAAGCAGTCCTCGGACAGCGAGCTCATGGCCCAGCAGCACCACGCC GCCTCTGCCGGGCTGGCCTCTGCCGCCGGGCCTGCCCGCCCTCGCTACCTCTTCCAGAGAAGGTCCAAG CTATGGGGGGACCCCGTGGAGAGCCGGGGGCTCCCTGGGCCTGAAGACGACAAACCAACTGTGATCAGT GAGCTCAGCTCCCGCCTGCAGCAGCTGAACAAGGACACGCGTTCCCTGGGGGAGGAACCAGTTGGTGGC CTGGGCAGCCTGCTGGACCCTGCCAAGAAGTCGCCCATCGCAGCAGCTCGGCTCTTCAGCAGCCTCGGT GAGCTGAGCTCCATTTCAGCGCAGCGCAGCCCCGGGGGCCCGGGCGGCGGGGCCTCGTACTCGGTGAGG CCCAGTGGCCGCTACCCCGTGGCGAGACGCGCCCCGAGCCCGGTGAAGCCCGCGTCGCTGGAGCGGGTG GAGGGGCTGGGGGCGGGCGCGGGGGGCGCAGGGCGGCCCTTCGGCCTCACGCCCCCCACCATCCTCAAG TCGTCCAGCCTCTCCATCCCGCACGAGCCCAAGGAGGTGCGCTTCGTGGTGCGCAGCGTGAGCGCGCGC AGTCGCTCCCCCTCGCCGTCGCCGCTGCCCTCGCCCGCGTCCGGCCCCGGCCCCGGCGCCCCCGGCCCA CGCCGACCCTTCCAGCAGAAGCCGCTGCAGCTCTGGAGCAAGTTCGACGTGGGCGACTGGCTGGAGAGC ATCCACCTAGGCGAGCACCGCGACCGCTTCGAGGACCATGAGATAGAAGGCGCGCACCTACCCGCGCTT ACCAAGGACGACTTCGTGGAGCTGGGCGTCACGCGCGTGGGCCACCGCATGAACATCGAGCGCGCGCTC AGGCAGCTGGACGGCAGCTGA
Restriction Sites	SgfI-NheI
ACCN	NM_033517
Insert Size	5196 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_033517.1
RefSeq Size	7113 bp
RefSeq ORF	5196 bp
Locus ID	85358
MW	184.7 kDa
Gene Summary	This gene is a member of the Shank gene family. Shank proteins are multidomain scaffold proteins of the postsynaptic density that connect neurotransmitter receptors, ion channels, and other membrane proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Shank proteins also play a role in synapse formation and dendritic spine maturation. Mutations in this gene are a cause of autism spectrum disorder (ASD), which is characterized by impairments in social interaction and communication, and restricted behavioral patterns and interests. Mutations in this gene also cause schizophrenia type 15, and are a major causative factor in the neurological symptoms of 22q13.3 deletion syndrome, which is also known as Phelan-McDermid syndrome. Additional isoforms have been described for this gene but they have not yet been experimentally verified. [provided by RefSeq, Mar 2012]

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