COLCA1 (NM_001302648) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C11orf92; CASC12; LOH11CR1F
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333712 representing NM_001302648. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGTCTTGCTCAGTCGCCCAGGCTGGAGTGCTAACCTCTCCTTTCATGTGGAGATGGACAGGGATG GCAGGAGCACTGAGTGCTCTTGACAACACCATTGAAGATGATGCTGACGATCAGCTACCCTGTGGAGAA GGCAGGCCAGGCTGGGTGAGAGGGGAGCTCCTTGGAAGTCAGGGGGTCTGTAAGGACAGCAAGGATCTC TTTGTCCCAACCTCCAGCAGCCTTTATGGGTGCTTCTGTGTTGGCCTTGTTTCTGGGATGGCCATCTCA GTGCTGTTGCTGGCTAGCGATTTCAGAAAACTAGATTTTTCTAGGCCTGAGCCCTGTTTTGAGAAAGAA GCTTCCCTCTGGTTTGTAGCTCAACATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302648
Insert Size	375 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302648.1
RefSeq Size	5372 bp
RefSeq ORF	375 bp
Locus ID	399948
UniProt ID	Q6ZS62
Protein Families	Transmembrane
MW	13.4 kDa
Gene Summary	This gene encodes a transmembrane protein that localizes to granular structures, including crystalloid eosinophilic granules and other granular organelles. This gene, along with an overlapping opposite strand gene, has been implicated as a susceptibility locus for colorectal cancer. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (6) contains an alternate 5' terminal exon and it thus differs in the 5' UTR, compared to variant 3. All variants (1 through 6) encode the same protein.

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