STX10 (NM_001271609) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hsyn10; SYN10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001271609, the custom clone sequence may differ by one or more nucleotides ATGTCTCTCGAAGACCCCTTTTTTGTAGTCCGAGGCGAGGTGCAGAAGGCGGTGAACACGGCCCGCGGGC TGTACCAGCGCTGGTGCGAGCTCCTGCAGGAAAGCGCGGCGGTCGGACGCGAGGAGCTGGACTGGACGAC CAATGAGCTGCGGAATGGCCTGCGCAGCATCGAGTGGGACCTCGAGGACCTGGAAGAGACCATCGGTATA GTGGAAGCCAACCCAGGCAAGTTCAAGCTCCCAGCCGGGGACCTGCAGGAGAGAAAGGTGTTCGTGGAGC GGATGCGAGAGGCAGTCCAGGAAATGAAGGACCATATGGTCAGCCCAACAGCCGTAGCATTTTTGGAGAG GAATAACAGAGAGATACTCGCAGGCAAGCCAGCTGCCCAGAAGTCACCCAGCGACCTGCTGGATGCCAGC GCAGTCTCGGCCACATCTCGCTACATCGAGGAGCAGCAGGCCACACAGCAGCTGATCATGGATGAACAGG ATCAACAGCTGGAGATGGTGTCTGGGAGCATCCAGGTTCTGAAGCACATGTCCGGCCGCGTTGGAGAAGA GCTGGACGAGCAGGCATGCTGGATGCCTTCGCCCAAGAGATGGACCACACCCAGTCCCGCATGGACGGGG TCCTCAGGAAGTTGGCCAAAGTATCCCACATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271609
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271609.1, NP_001258538.1
RefSeq Size	1316 bp
RefSeq ORF	663 bp
Locus ID	8677
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	SNARE interactions in vesicular transport
Gene Summary	This gene belongs to the syntaxin family and encodes a soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE). The encoded protein is involved in docking and fusion events at the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1.

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