RNF35 (TRIM40) (NM_001286633) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RNF35
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286633, the custom clone sequence may differ by one or more nucleotides ATGATCCCTTTGCAGAAGGACAACCAGGAGGAGGGTGTCTGCCCCATCTGCCAGGAGAGCCTGAAGGAGG CCGTGAGCACCAACTGCGGACATCTCTTCTGTCGAGTGTGCCTGACACAGCATGTGGAGAAGGCCTCAGC CTCTGGGGTCTTCTGCTGCCCCCTCTGCCGGAAGCCCTGTTCTGAGGAGGTGCTAGGGACAGGCTATATC TGCCCCAACCACCAGAAGAGGGTGTGCAGGTTCTGTGAGGAGAGCAGACTTCTTCTATGTGTGGAATGCC TGGTGTCCCCTGAACACATGTCTCATCATGAACTGACCATTGAAAATGCCCTCAGCCACTACAAGGAACG ACTCAATCGCCGGAGCAGGAAGCTCAGAAAGGACATTGCAGAACTTCAGCGGCTCAAGGCTCAGCAGGAG AAGAAACTGCAGGCTCTGCAGTTTCAGGTAGACCACGGGAACCACAGGCTGGAGGCTGGGCCGGAGAGCC AGCACCAAACCAGGGAACAGCTGGGTGCCCTCCCTCAGCAGTGGCTGGGCCAGCTGGAGCACATGCCAGC AGAAGCGGCCAGAATCCTTGACATCTCCAGGGCAGTAACACAGCTCAGAAGCCTGGTCATTGATCTGGAA AGGACGGCCAAGGAATTAGACACCAACACACTGAAGAATGCTGGTGACTTACTGAACAGGAGTGCTCCAC AGAAATTAGAGGTTATTTATCCCCAGTTGGAGAAAGGAGTCAGTGAATTGCTTCTTCAGCCCCCTCAGAA GCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286633
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286633.1, NP_001273562.1
RefSeq Size	2092 bp
RefSeq ORF	777 bp
Locus ID	135644
UniProt ID	Q6P9F5
Gene Summary	This gene encodes a member of the tripartite motif (TRIM) protein family. The encoded protein may play a role as a negative regulator against inflammation and carcinogenesis in the gastrointestinal tract. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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