TFII I (GTF2I) (NM_001280800) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BAP135; BTKAP1; DIWS; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001280800, the custom clone sequence may differ by one or more nucleotides ATGGCCCAAGTTGCAATGTCCACCCTCCCCGTTGAAGATGAGGAGTCCTCGGAGAGCAGGATGGTGGTGA CATTCCTCATGTCAGCTCTCGAGTCCATGTGTAAAGAACTGGCCAAGTCCAAAGCCGAAGTGGCCTGCAT TGCAGTGTATGAAACAGACGTGTTTGTCGTCGGAACTGAAAGAGGACGTGCTTTTGTCAATACCAGAAAG GATTTTCAAAAAGATTTTGTAAAATATTGTGTTGAAGAAGAAGAAAAAGCTGCAGAGATGCATAAAATGA AATCTACAACCCAGGCAAATCGGATGAGTGTAGATGCTGTAGAAATTGAAACACTCAGAAAAACAGTTGA GGACTATTTCTGCTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTACCTGTACCATATGAGAAG ATGCTGCGAGACCAGTCGGCTGTGGTAGTGCAGGGGCTTCCGGAAGGTGTTGCCTTTAAACACCCCGAGA ACTATGATCTTGCAACCCTGAAATGGATTTTGGAGAACAAAGCAGGGATTTCATTCATCATTAAGAGACC TTTTTTAGAGCCAAAGAAGCATGTAGGTGGTCGTGTGATGGTAACAGATGCTGACAGGTCAATACTATCT CCAGGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAACCCACAGAAGATTCTGGCATTTCCCTGGAAA TGGCAGCTGTGACAGTAAAGGAAGAATCAGAAGATCCTGATTATTATCAATATAACATTCAAGGAAGCCA CCATTCTTCAGAGGGCAATGAAGGCACAGAAATGGAAGTACCAGCAGAAGGTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001280800
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001280800.1, NP_001267729.1
RefSeq Size	1377 bp
RefSeq ORF	825 bp
Locus ID	2969
UniProt ID	P78347
Protein Families	Transcription Factors
Protein Pathways	Basal transcription factors
Gene Summary	This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (6) lacks multiple coding exons and its transcription extends past a splice site used in variant 1, resulting in a distinct 3' coding region and 3' UTR. The encoded isoform (6) is shorter and has a distinct C-terminus, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.