C5ORF33 (NADK2) (NM_001287340) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C5orf33; DECRD; MNADK; NADKD1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001287340, the custom clone sequence may differ by one or more nucleotides ATGCTGCTGGCAGCGAGTAAAGTCTTGGACAGACTTAAACCAGTTATAGGGGTAAACACTGATCCAGAAC GGTCTGAGGGTCATTTATGCCTGCCCGTTCGATATACACATTCCTTTCCAGAAGCCTTACAGAAGTTCTA TCGTGGTGAGTTCAGGTGGTTGTGGAGGCAGAGAATCAGGTTATACCTTGAAGGGACTGGCATAAACCCT GTACCTGTGGACCTTCACGAGCAGCAGCTAAGCTTGAATCAGCACAATAGAGCCCTTAACATTGAAAGAG CTCATGATGAAAGGTCTGAGGCTTCAGGACCCCAACTTCTGCCAGTGAGAGCACTAAATGAAGTCTTCAT TGGGGAGAGTCTGTCATCCAGGGCTTCCTACTATGAGATTTCAGTTGATGATGGTCCATGGGAAAAACAG AAGAGTTCAGGGCTCAATTTGTGTACTGGAACAGGATCAAAGGCCTGGTCATTCAATATTAACAGGGTTG CAACTCAGGCTGTAGAAGATGTTTTAAATATTGCAAAACGACAAGGAAATTTGAGTCTTCCATTGAACAG AGAATTGGTAGAGAAAGTAACAAATGAATATAATGAATCACTGCTCTACAGTCCGGAAGAACCAAAAATA CTTTTCAGTATTCGAGAACCAATAGCAAATAGAGTTTTCTCAAGCAGTCGTCAGCGTTGTTTCTCCTCAA AGGTTTGTGTTCGTTCTCGTTGTTGGGATGCCTGTATGGTTGTGGATGGAGGAACTTCTTTTGAGTTTAA TGATGGTGCAATTGCTTCGATGATGATCAATAAAGAAGATGAGCTTCGAACTGTGCTTCTTGAACAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001287340
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287340.1, NP_001274269.1
RefSeq Size	3630 bp
RefSeq ORF	840 bp
Locus ID	133686
UniProt ID	Q4G0N4
Gene Summary	This gene encodes a mitochondrial kinase that catalyzes the phosphorylation of NAD to yield NADP. Mutations in this gene result in 2,4-dienoyl-CoA reductase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (3) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform.

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